| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,622,878 | T→C | 9.4% | G393G (GGT→GGC) | ppk → | polyphosphate kinase, component of RNA degradosome |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,622,878 | 0 | T | C | 9.4% | 174.3 / 9.6 | 64 | G393G (GGT→GGC) | ppk | polyphosphate kinase, component of RNA degradosome |
| Reads supporting (aligned to +/- strand): ref base T (27/31); new base C (3/3); total (30/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
AATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGA > W3110S.gb/2622809‑2622945 | aaTTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGt < 1:2195466/70‑1 (MQ=255) tACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCa < 1:4861754/70‑1 (MQ=255) aCCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGCAAGAAAGTCAc < 1:496232/70‑1 (MQ=255) cgcgTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCAc < 1:2059632/68‑1 (MQ=255) cgcgTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgt > 1:2005954/1‑71 (MQ=255) cgTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtgg < 1:231293/71‑1 (MQ=255) gTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtggt < 1:4665979/71‑1 (MQ=255) tGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAg < 1:490434/56‑1 (MQ=255) tGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCa < 1:5118077/63‑1 (MQ=255) tGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtg < 1:4715710/68‑1 (MQ=255) tGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtg < 1:461334/68‑1 (MQ=255) cGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGtt < 1:4455884/71‑1 (MQ=255) aGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGaaa > 1:2414101/1‑52 (MQ=255) aGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAg < 1:13771/53‑1 (MQ=255) gATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGtt < 1:312507/71‑1 (MQ=255) ttCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGt > 1:930472/1‑68 (MQ=255) tCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTa > 1:4496536/1‑69 (MQ=255) cACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTggtggt > 1:2612982/1‑61 (MQ=255) gCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGgcg < 1:4539970/71‑1 (MQ=255) tcatcGACTCGATGATCCACGCCGCACATAACGGt < 1:1570239/35‑1 (MQ=255) tcatcGACTCGATGATCCACGCCGCACATAACGGTAAGa < 1:593511/39‑1 (MQ=255) tcatcGACTCGATGATCCACGCCGCACATAACGGTAAGa < 1:3046351/39‑1 (MQ=255) tcatcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtg < 1:3689432/50‑1 (MQ=255) tcatcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtg < 1:3518405/50‑1 (MQ=255) atcGACTCGATGATCCACGCCGCACATAACGGTaa > 1:2901876/1‑35 (MQ=255) atcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAg > 1:4232991/1‑40 (MQ=255) atcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGt > 1:370154/1‑41 (MQ=255) atcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCg > 1:4611720/1‑46 (MQ=255) atcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCg > 1:2043623/1‑46 (MQ=255) atcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGttt > 1:1668428/1‑71 (MQ=255) atcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGttt > 1:1525256/1‑71 (MQ=255) atcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGtt < 1:4864666/70‑1 (MQ=255) tcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtg < 1:4343761/47‑1 (MQ=255) tcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgtg < 1:1429259/47‑1 (MQ=255) tcGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTc > 1:1926343/1‑71 (MQ=255) cGACTCGATGATCCACTCCGCACATAACGGTAAGAAAGTCACCgtg < 1:2758247/46‑1 (MQ=255) cGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCAc > 1:3440245/1‑42 (MQ=255) gACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGtt > 1:4790100/1‑67 (MQ=255) gACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTcga > 1:2395546/1‑71 (MQ=255) tCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgt < 1:1927919/41‑1 (MQ=255) tCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgt < 1:2102739/41‑1 (MQ=255) tCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCgt < 1:1501366/41‑1 (MQ=255) cGATGATCCACGCCGCACATAACGGTAAGAAAGTCAc > 1:3664938/1‑37 (MQ=255) cGATGATCCACGCCGCACATAACGGTAAGAAAGTCAc > 1:138806/1‑37 (MQ=255) cGATGATCCACGCCGCACATAACGGTAAGAAAGTCAc > 1:4847571/1‑37 (MQ=255) gatgatCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTa > 1:820751/1‑52 (MQ=255) gatCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTAc > 1:3276129/1‑50 (MQ=255) tCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGcc < 1:1382205/71‑1 (MQ=255) cACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCa > 1:4945467/1‑70 (MQ=255) aCGCCGCACATAACGGTACGAAAGTCACCGTGGTGGTTGAGTTACAGgcg < 1:1032955/50‑1 (MQ=255) cgccgcACATAACGGCAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACa < 1:41086/71‑1 (MQ=255) gcACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCa < 1:2125389/71‑1 (MQ=255) cacaTAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTcgacg < 1:3258426/54‑1 (MQ=255) acaTAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAgg < 1:3974335/41‑1 (MQ=255) acaTAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAgg < 1:23785/41‑1 (MQ=255) caTAACGGTAAGAAAGTCACCGTGGTTGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTg < 1:1796936/71‑1 (MQ=255) aTAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGgcg > 1:2716849/1‑41 (MQ=255) aTAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGgcg > 1:1154204/1‑41 (MQ=255) tAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTcga > 1:1062260/1‑48 (MQ=255) tAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACAtt < 1:1671134/64‑1 (MQ=255) tAACGGCAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTcgacg > 1:4371150/1‑50 (MQ=255) tAACGGCAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCa > 1:1110987/1‑66 (MQ=255) tAACGGCAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCa > 1:502231/1‑66 (MQ=255) aaCGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTggg > 1:4684899/1‑70 (MQ=255) aaCGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGc > 1:612276/1‑71 (MQ=255) cGGCAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGa < 1:29755/71‑1 (MQ=255) tAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCaa > 1:2311902/1‑54 (MQ=255) | AATTAACATTTACCGCGTGGCGAAAGATTCACGCATCATCGACTCGATGATCCACGCCGCACATAACGGTAAGAAAGTCACCGTGGTGGTTGAGTTACAGGCGCGTTTCGACGAAGAAGCCAACATTCACTGGGCGA > W3110S.gb/2622809‑2622945 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |