| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,678,206 | C→T | 32.4% | A1066T (GCT→ACT) | yphG ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,678,206 | 0 | C | T | 32.4% | 69.1 / 46.7 | 56 | A1066T (GCT→ACT) | yphG | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (20/16); new base T (10/8); total (31/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
TCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAG > W3110S.gb/2678149‑2678269 | tCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGTGCTAGCAGACTCa < 1:1570892/71‑1 (MQ=255) aCGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCata > 1:3406095/1‑71 (MQ=255) aCGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCata > 1:2573025/1‑71 (MQ=255) cGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCatat < 1:3459901/71‑1 (MQ=255) gtggtgAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGa > 1:398513/1‑57 (MQ=255) gtgAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGTGCGAGCAGACTCATATTgcgc < 1:806907/71‑1 (MQ=255) tgAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGTGCGAGCAGACTCATATTGCGCa < 1:4589996/71‑1 (MQ=255) tgAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCa < 1:493854/62‑1 (MQ=255) tgAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCa < 1:1267391/71‑1 (MQ=255) gAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGa > 1:3213971/1‑52 (MQ=255) gAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGa > 1:2640090/1‑52 (MQ=255) aataatGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGTGCGAGCAGACTCATATTGCGCAAc > 1:3919059/1‑71 (MQ=255) aataatGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGTGCGAGCAGACTCATATTGCGCAAc > 1:4704320/1‑71 (MQ=255) ataatGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGTGCGAGCAGACTAATATTGCGCAAcc > 1:2967704/1‑71 (MQ=255) ataatGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGAGCGAGCAGACTCa < 1:4195216/59‑1 (MQ=255) taatGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGTGCGAGCAGa > 1:3376696/1‑54 (MQ=255) taatGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCa < 1:4619311/52‑1 (MQ=255) taatGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCa < 1:4594828/52‑1 (MQ=255) taatGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCa < 1:2088675/52‑1 (MQ=255) taatGCCGCTTTCGG‑CAAGCCGGATTAATCGCCGTCAATTCAGTGCGAGCAGa > 1:3114381/1‑53 (MQ=255) aatGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAc > 1:422012/1‑68 (MQ=255) aatGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAc > 1:1150000/1‑68 (MQ=255) aatGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAAc > 1:4531765/1‑68 (MQ=255) atGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTgcgc < 1:701583/64‑1 (MQ=255) ccGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTcccc > 1:2598367/1‑70 (MQ=255) ccGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCg < 1:4342323/71‑1 (MQ=255) ccGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCg < 1:178361/71‑1 (MQ=255) ccGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCg < 1:4872786/71‑1 (MQ=255) tttCGGCCAAGCCGGATTAATCGCCGTCAATTCAGcgcg > 1:1767870/1‑39 (MQ=255) tttCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTc > 1:2957438/1‑71 (MQ=255) tttCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGAATCa < 1:4500042/49‑1 (MQ=255) tttCGGCCAAGCCGGATTAATCGCCGTCAATTCAGAGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTc > 1:1819569/1‑71 (MQ=255) tCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTccc > 1:666270/1‑71 (MQ=255) gCCAAGCCGGATTAATCGCCGTCAATTCAGTGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCcgcg > 1:3685318/1‑71 (MQ=255) gCCAAGCCGGATTAATCGCCGTCAATTCAGTGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCcgcg < 1:4150872/71‑1 (MQ=255) cAAGCCGGATTAATCGCCGTCAATTCAGTGCGAGCAGACTc < 1:1367714/41‑1 (MQ=255) cAAGCCGGATTAATCGCCGTCAATTCAGTGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGa > 1:1314911/1‑71 (MQ=255) cGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGc > 1:3403774/1‑69 (MQ=255) ggATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAg < 1:1272588/71‑1 (MQ=255) aTTAATCGCCGTCAATTCAGTGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCa < 1:2453185/71‑1 (MQ=255) ttAATCGCCGTCAATTCAGTGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCAt > 1:3114124/1‑71 (MQ=255) aaTCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCa > 1:970112/1‑68 (MQ=255) aaTCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCa > 1:3471937/1‑68 (MQ=255) aaTCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGCCCCGCGGACGCCAGCATc < 1:3404222/70‑1 (MQ=255) gCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGccac > 1:46800/1‑71 (MQ=255) gTCAATTCAGTGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGAGGACGCCAGCATCGCCACCAt > 1:182928/1‑71 (MQ=255) tCAATTCAGTGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTccc < 1:3576280/47‑1 (MQ=255) tCAATTCAGTGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTccc < 1:4309522/47‑1 (MQ=255) tCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGc < 1:4057288/59‑1 (MQ=255) tCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGc < 1:4896809/59‑1 (MQ=255) tCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCAt > 1:1885731/1‑70 (MQ=255) cAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGt < 1:3802756/43‑1 (MQ=255) aaTTCAGTGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAg > 1:3817303/1‑71 (MQ=255) aaTTCAGCGCGATCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAg > 1:5120031/1‑71 (MQ=255) aaTTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGCCGCCAGCATCGCCACCATCAg > 1:3318534/1‑71 (MQ=255) cAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGa > 1:2898457/1‑36 (MQ=255) aGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCAt > 1:3208097/1‑54 (MQ=255) | TCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAG > W3110S.gb/2678149‑2678269 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |