| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,680,577 | C→A | 43.0% | L275L (CTG→CTT) | yphG ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,680,577 | 0 | C | A | 43.0% | 86.5 / 69.8 | 72 | L275L (CTG→CTT) | yphG | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (29/12); new base A (16/15); total (45/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GGCCAAATTCACTGTGTCCCCAACTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATA > W3110S.gb/2680510‑2680645 | ggCCAAATTCACTGTGTCCCCAACTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTgaag > 1:582696/1‑70 (MQ=255) aaaTTCACTGTGTCCCCAACTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCAAAc > 1:792448/1‑71 (MQ=255) aaaTTCACTGTGTCCCCAACTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCAAAc > 1:3443014/1‑71 (MQ=255) aaaTTCACTGTGTCCCCAACTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCAAAc > 1:3248508/1‑71 (MQ=255) aTTCACTGTGTCCCCAACTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCg > 1:3980541/1‑71 (MQ=255) aTTCACTGTGTCCCCAACTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCg > 1:1941361/1‑71 (MQ=255) aTTCACTGTGTCCCCAACTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCAACCCg > 1:3780329/1‑71 (MQ=255) ttCACTGTGTCCCCAACTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGc > 1:953088/1‑71 (MQ=255) ttCACTGTGTCCCCAACTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGc > 1:2175805/1‑71 (MQ=255) ttCACTGTGTCCCCAACTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCAAACCGc > 1:1052206/1‑71 (MQ=255) cACTGTGTCCCCAACTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCa > 1:1029956/1‑71 (MQ=255) tgtgTCCCCAACTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGcc < 1:3434441/67‑1 (MQ=255) tgtCCCCAACTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCtt < 1:3472118/70‑1 (MQ=255) tgtCCCCAACTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCaaa > 1:1697967/1‑60 (MQ=255) tCCCCAACTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCAAACCGCCATCTTCAt > 1:1999354/1‑71 (MQ=255) ccAACTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGt < 1:4166051/71‑1 (MQ=255) ccAACTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGt < 1:344284/71‑1 (MQ=255) ccAACTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCAAACCGCCATCTTCATCGt < 1:533159/71‑1 (MQ=255) ccAACTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCAAACCGCCATCTTCATCGt < 1:3832247/71‑1 (MQ=255) aaCTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTGGGCAACGTGAAGCAAACCGCCATCTTCATCGTGa < 1:2385756/71‑1 (MQ=255) aCTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCaa < 1:2700917/51‑1 (MQ=255) aCTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGac < 1:4144821/71‑1 (MQ=255) aCTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCAAACCGCCATc < 1:4487924/60‑1 (MQ=255) aCTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCAAACCGCCATc < 1:1047614/60‑1 (MQ=255) aCTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCAAACCGCCATc < 1:620314/60‑1 (MQ=255) cTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAAc > 1:3940475/1‑52 (MQ=255) cTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAAc > 1:3632806/1‑52 (MQ=255) tCCACTGTTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCaa < 1:2408577/50‑1 (MQ=255) tCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGacac > 1:1057167/1‑71 (MQ=255) cACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGAcacca > 1:3159737/1‑71 (MQ=255) cACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCaaa > 1:4047812/1‑48 (MQ=255) cACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCaa > 1:79518/1‑47 (MQ=255) cACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCAAACCGCCATCTTCATCGTGacac < 1:3642775/69‑1 (MQ=255) cACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCAAACCGCCATCTTCATCGTGaaac < 1:2126103/69‑1 (MQ=255) cTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCAcg < 1:2782/71‑1 (MQ=255) cTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCAAACCGCCATCTTCATCGTGACACCAcg > 1:3599423/1‑71 (MQ=255) tG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCAAACCGCCATc < 1:5023988/53‑1 (MQ=255) tG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCAAACCGCCATc < 1:2854511/53‑1 (MQ=255) tG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCAAACCGCCATCtt < 1:2145171/55‑1 (MQ=255) ttttttACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCAAACCGCCATCtt > 1:711096/1‑53 (MQ=255) ttttACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGcc > 1:2540051/1‑70 (MQ=255) ttttACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGcc > 1:3735424/1‑70 (MQ=255) ttttACCTGGCGCAATATGGTGGTTGGCAACGTGAAGCAAACCGCCATCTTCATCGTGac > 1:2374563/1‑60 (MQ=255) ccTGGCGCTATATGGTGGGTGGCAACGTGAAGCAAACCGCCATc < 1:1934349/44‑1 (MQ=38) ggCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATc > 1:3149657/1‑71 (MQ=255) gcgcAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACaaa < 1:4950432/67‑1 (MQ=255) gcgcAATATGGTGGTTGGCAACGTGAAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCg < 1:3770110/71‑1 (MQ=255) gcgcAATATGGTGGTTGGCAACGTGAAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCg < 1:3257703/71‑1 (MQ=255) aaTATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCg < 1:4390494/67‑1 (MQ=255) atatGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGt < 1:2117094/43‑1 (MQ=255) atGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGt > 1:2490872/1‑41 (MQ=255) atGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGt > 1:525234/1‑41 (MQ=255) atGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGa > 1:4652885/1‑43 (MQ=255) atGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGc > 1:4849550/1‑70 (MQ=255) atGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGc > 1:4104167/1‑70 (MQ=255) atGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGc > 1:2031961/1‑70 (MQ=255) atGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATAGTGACACCACGCGCCGACAAAATCGTACTGc > 1:3435352/1‑70 (MQ=255) atGGTGGTTGGCAACGTGAAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGc > 1:3432101/1‑70 (MQ=255) tggtggTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGa > 1:1729743/1‑42 (MQ=255) tggtggTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGa > 1:792753/1‑42 (MQ=255) tggtggTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGa > 1:2931759/1‑42 (MQ=255) tggtggTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAAt > 1:3517024/1‑61 (MQ=255) tggtggTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGa > 1:388527/1‑71 (MQ=255) tggtTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGAttt < 1:3826056/71‑1 (MQ=255) gtTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTa > 1:1406440/1‑60 (MQ=255) gtTGGCAACGTGCAGCAAACCGCCATCCTCATCGTGACACCACGCGCCGACAAAATCGTa > 1:1006897/1‑60 (MQ=255) ggCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACaaaa > 1:2822225/1‑52 (MQ=255) gCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGc < 1:4473730/71‑1 (MQ=255) gTGAAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTa > 1:4615205/1‑51 (MQ=255) gTGAAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTa > 1:408669/1‑51 (MQ=255) gTGAAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCata > 1:3499457/1‑70 (MQ=255) tGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCatata > 1:2124814/1‑71 (MQ=255) tGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCatata > 1:244556/1‑71 (MQ=255) | GGCCAAATTCACTGTGTCCCCAACTCCACTG‑TTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATA > W3110S.gb/2680510‑2680645 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |