| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,695,708 | C→T | 33.8% | L372F (CTT→TTT) | yfhD → | predicted transglycosylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,695,708 | 0 | C | T | 33.8% | 86.6 / 65.0 | 71 | L372F (CTT→TTT) | yfhD | predicted transglycosylase |
| Reads supporting (aligned to +/- strand): ref base C (21/26); new base T (11/13); total (32/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
CCCGCGCCCTGACGGCAAAAACCAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTGGACATGAAGCCTACGCT > W3110S.gb/2695637‑2695773 | cccGCGCCCTGACGGCAAAAACCAAAGGGAAT‑CTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTAc > 1:4597873/1‑71 (MQ=255) gcgcCCTGACGGCAAAAACCAACGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTAttt < 1:1011928/71‑1 (MQ=255) cgcCCTGACGGCAAAAACCAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTa < 1:2755317/71‑1 (MQ=255) gcCCTGACGGCAAAAACCAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTa < 1:5126408/70‑1 (MQ=255) ccTGACGGCAAAAACCAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTAGc > 1:1911463/1‑70 (MQ=255) cTGACGGCAAAAACCAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACtt > 1:3432992/1‑66 (MQ=255) cTGACGGCAAAAACCAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTAGc > 1:3331874/1‑69 (MQ=255) gACGGCAAAAACCAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTTGCCAg > 1:2547891/1‑70 (MQ=255) gACGGCAAAAACCAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAg > 1:161212/1‑70 (MQ=255) aCGGCAAAAACCAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAGa < 1:4341567/70‑1 (MQ=255) gCAAAAACCAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAGAAAcc < 1:21572/71‑1 (MQ=255) cAAAAACCAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTATTTAGCCAGAAAccc > 1:874980/1‑71 (MQ=255) aaaaCCAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTATTTAGCCAGAAACCCTa > 1:148547/1‑71 (MQ=255) aaaaCCAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCTa > 1:1476423/1‑71 (MQ=255) aaaaCCAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCTa < 1:2103305/71‑1 (MQ=255) aaaCCAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTATTTAGc < 1:1235602/59‑1 (MQ=255) aaaCCAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCTAt < 1:4901684/71‑1 (MQ=255) aaaCCAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCTAt < 1:4791759/71‑1 (MQ=255) aaCCAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTATTTAGCCAGAAACCCTAt > 1:3697597/1‑70 (MQ=255) ccAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAGAAAccc < 1:4852661/65‑1 (MQ=255) ccAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAGAAAccc < 1:3180293/65‑1 (MQ=255) ccAAAGCGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAGAAAccc < 1:2433966/65‑1 (MQ=255) cAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAGaa > 1:4189318/1‑60 (MQ=255) aGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCTATTACAGCa < 1:394680/71‑1 (MQ=255) aGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCTATTACAGCa < 1:3431341/71‑1 (MQ=255) gAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTATTTAGCCAGAAACCCTATTACAGCAAGc < 1:4127183/71‑1 (MQ=255) cTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCTATTACAGCAAGCTGACt > 1:3953291/1‑71 (MQ=255) cTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCTATTACAGCAAGCTGACt > 1:104117/1‑71 (MQ=255) gCTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCt < 1:476740/43‑1 (MQ=255) gCTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCt < 1:2879714/43‑1 (MQ=255) gCTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCt < 1:1784421/43‑1 (MQ=255) cTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCt < 1:1974823/71‑1 (MQ=255) aCGTAAAACAGCGTCTGCCTTTATTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCg < 1:5123547/70‑1 (MQ=255) aCGTAAAACAGCGTCTGCCTTTATTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGt > 1:439158/1‑71 (MQ=255) aCGTAAAACAGCGTCTGCCTTTATTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGt > 1:112804/1‑71 (MQ=255) aCGTAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCTATTACAGCAAGCt < 1:3768346/52‑1 (MQ=255) cGTAAAACAGCGTCTGCCTTTATTTAGCCAGAAACCCTATTa > 1:4702826/1‑42 (MQ=255) cGTAAAACAGCGTCTGCCTTTATTTAGCCAGAAACCCTATTa > 1:5042091/1‑42 (MQ=255) cGTAAAACAGCGTCTGCCTTTATTTAGCCAGAAACCCTATTa > 1:998783/1‑42 (MQ=255) cGTAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTg < 1:3205072/71‑1 (MQ=255) gTAAAACAGCGTCTGCCTTTATTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCg > 1:3193131/1‑68 (MQ=255) gTAAAACAGCGTCTGCCTTTATTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCg > 1:2412447/1‑68 (MQ=255) gTAAAACAGCGTCTGCCTTTATTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTgg < 1:173888/71‑1 (MQ=255) gTAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCTa > 1:3332929/1‑38 (MQ=255) gTAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCTATTACAGCAAGCTGa > 1:1308051/1‑52 (MQ=255) gTAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCTATTACAGCAAGCTGa > 1:3923019/1‑52 (MQ=255) tAAAACAGCGTCTGCCTTTATTTAGCCAGAAACCCTATTACAGCAAGCTg < 1:4578695/50‑1 (MQ=255) tAAAACAGCGTCTGCCTTTATTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTGGa < 1:2929498/71‑1 (MQ=255) tAAAACAGCGTCTGCCTTTATTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTGGa < 1:2142563/71‑1 (MQ=255) tAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCTATTACAGCaa < 1:5072481/46‑1 (MQ=255) tAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTGGa < 1:4628694/71‑1 (MQ=255) tAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTGGa < 1:632859/71‑1 (MQ=255) tAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTGGa < 1:3155116/71‑1 (MQ=255) aaaCAGCGTCTGCCTTTACTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTGGACa > 1:1714094/1‑71 (MQ=255) aaCAGCGTCTGCCTTTATTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCt < 1:4797070/62‑1 (MQ=255) aCAGCGTCTGCCTTTATTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTGGACAt < 1:2360951/70‑1 (MQ=255) aCAGCGTCTGCCTTTACTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTGGACATg < 1:2638913/71‑1 (MQ=255) aCAGCGTCTGCCTTTACTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTGGACATg < 1:62010/71‑1 (MQ=255) cGTCTGCCTTTACTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTGGACATGAAGc > 1:4400998/1‑71 (MQ=255) gTCTGCCTTTACTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTGGACATGAAGcc > 1:4495582/1‑71 (MQ=255) tCTGCCTTTATTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTGGACa > 1:4026104/1‑63 (MQ=255) cTGCCTTTATTTAGCCAGAAACCCTATTACAGCAAGCTGACt < 1:4392436/42‑1 (MQ=255) gCCTTTACTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTGGACATGAAGCCTACg > 1:2499719/1‑71 (MQ=255) cTTTATTTAGCCAGAAGCCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTGGACATGAAGCCTACGCt < 1:2166947/71‑1 (MQ=255) cTTTATTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTGGACATGAAGCCTACGCt < 1:2936279/71‑1 (MQ=255) cTTTATTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTGGACATGAAGCCTACGCt < 1:586390/71‑1 (MQ=255) cTTTACTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTGGACATGAAGCCTACGCt < 1:1311093/71‑1 (MQ=255) ttACTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTGGACATGAAGCCTACGCt < 1:2746567/69‑1 (MQ=255) ttACTAAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTc > 1:3295468/1‑49 (MQ=255) tACTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTa > 1:2823186/1‑43 (MQ=255) tACTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTa > 1:4910324/1‑43 (MQ=255) tACTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTa > 1:2422944/1‑43 (MQ=255) tACTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTa > 1:2645765/1‑43 (MQ=255) | CCCGCGCCCTGACGGCAAAAACCAAAGGGAATCCTGACAGTTGGGCTGACGTAAAACAGCGTCTGCCTTTACTTAGCCAGAAACCCTATTACAGCAAGCTGACTTACGGCTACGCTCGTGGACATGAAGCCTACGCT > W3110S.gb/2695637‑2695773 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |