| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,719,037 | G→A | 36.7% | V143V (GTG→GTA) | yfiQ → | fused predicted acyl‑CoA synthetase NAD(P)‑binding subunit and ATP‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,719,037 | 0 | G | A | 36.7% | 26.6 / 26.8 | 30 | V143V (GTG→GTA) | yfiQ | fused predicted acyl‑CoA synthetase NAD(P)‑binding subunit and ATP‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base G (13/6); new base A (4/7); total (17/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCG > W3110S.gb/2718975‑2719101 | ggACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTa < 1:3270400/70‑1 (MQ=255) gACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTaa < 1:4036540/70‑1 (MQ=255) cAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCg > 1:2650862/1‑58 (MQ=255) cAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGa > 1:4728780/1‑59 (MQ=255) tACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGtt > 1:981345/1‑70 (MQ=255) cTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTa < 1:3068547/70‑1 (MQ=255) ccTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCaa < 1:3115824/71‑1 (MQ=255) ccTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCa < 1:3802332/70‑1 (MQ=255) cTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAAt > 1:2455381/1‑71 (MQ=255) cTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAAt > 1:2813967/1‑71 (MQ=255) gggaaGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCg > 1:2916262/4‑71 (MQ=255) ggCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCg > 1:410179/1‑71 (MQ=255) ggCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCg > 1:3910371/1‑71 (MQ=255) gCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCgt < 1:2886899/71‑2 (MQ=255) gCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCgg < 1:3444869/71‑1 (MQ=255) gCAAGGTCTGAATGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCgg < 1:121748/71‑1 (MQ=255) cTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCg > 1:1263370/1‑70 (MQ=255) tGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGtc > 1:4943891/1‑71 (MQ=255) aaTGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGtctc > 1:764537/1‑71 (MQ=255) aaTGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGt < 1:32639/68‑1 (MQ=255) aaTGCCAGCTTTTCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGtct < 1:2155499/70‑1 (MQ=255) aTGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTcc < 1:507847/71‑1 (MQ=255) aTGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTcc < 1:3613231/71‑1 (MQ=255) gCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACAcc < 1:4121058/71‑1 (MQ=255) cTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTAt > 1:4274507/1‑42 (MQ=255) cTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAAt > 1:3122016/1‑50 (MQ=255) cTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAAt > 1:1048583/1‑50 (MQ=255) tttCGCCTGTACCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCaa > 1:2376308/1‑64 (MQ=255) ttCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCaa > 1:731871/1‑63 (MQ=255) cTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAaca > 1:268310/1‑60 (MQ=255) tgtgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCg < 1:1516667/68‑1 (MQ=255) gtgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTc < 1:556674/66‑1 (MQ=255) | GGACCAAACAGTCTGGGTTTACTGGCTCCCTGGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCG > W3110S.gb/2718975‑2719101 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |