| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,771,011 | C→T | 6.5% | G111S (GGC→AGC) | ypjL ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,771,011 | 0 | C | T | 6.5% | 248.9 / 5.5 | 77 | G111S (GGC→AGC) | ypjL | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (35/37); new base T (2/3); total (37/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01 | |||||||||||
GCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGC > W3110S.gb/2770941‑2771080 | gCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGcc < 1:2175522/71‑1 (MQ=255) aaGCACATGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCTaa > 1:16624/1‑71 (MQ=255) aaGCACATGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCaa > 1:2490833/1‑71 (MQ=255) acaTGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATcac < 1:4290664/71‑1 (MQ=255) acaTGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATcac < 1:2373966/71‑1 (MQ=255) aTGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCTAATc < 1:4113671/67‑1 (MQ=255) cAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGcc < 1:1104674/60‑1 (MQ=255) gCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCaa > 1:2234996/1‑60 (MQ=255) gCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCaa > 1:2737782/1‑60 (MQ=255) ccACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGcc < 1:3221180/57‑1 (MQ=255) cacaGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATa < 1:3649908/71‑1 (MQ=255) cacaGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATa < 1:4483354/71‑1 (MQ=255) acaGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCaa > 1:720793/1‑57 (MQ=255) acaGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATca > 1:5077023/1‑60 (MQ=255) acaGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATca > 1:311869/1‑60 (MQ=255) acaGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATaa < 1:3499664/71‑1 (MQ=255) acaGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATaa < 1:2821427/71‑1 (MQ=255) acaGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATaa < 1:2133039/71‑1 (MQ=255) acaGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATa > 1:3513462/1‑70 (MQ=255) acaGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATa > 1:4099999/1‑70 (MQ=255) agcagAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAAt < 1:327640/71‑1 (MQ=255) gcagAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAAt > 1:179343/1‑70 (MQ=255) gcagAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATc < 1:3467881/71‑1 (MQ=255) cagAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCa > 1:4579628/1‑71 (MQ=255) gTTGCCAGACTGCCAATCGGGGTGATTTTCGTGCCTAAATCACAGCCAATCACAtt < 1:4484966/56‑1 (MQ=255) gTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGc > 1:5051788/1‑71 (MQ=255) ttGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcc > 1:4108154/1‑71 (MQ=255) ttGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcc > 1:2833311/1‑71 (MQ=255) ttGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcc > 1:4114043/1‑71 (MQ=255) gCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATaaca > 1:2901388/1‑51 (MQ=255) gCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCctc > 1:671366/1‑71 (MQ=255) cAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCAtt > 1:4344988/1‑64 (MQ=255) gACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAAt < 1:2136887/43‑1 (MQ=255) gACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCctct > 1:3066410/1‑68 (MQ=255) gACAGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCctct > 1:2368065/1‑68 (MQ=255) tggcAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGc > 1:1732160/4‑61 (MQ=255) tGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGc > 1:778402/1‑61 (MQ=255) ccAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTgacgac > 1:4107763/1‑71 (MQ=255) aaTCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGc < 1:4415277/71‑1 (MQ=255) aTCGGGGTGATTTTCGGGCCTAAATCACAGCTAATCACATTGGCATAAATCATTGCCTCTTTgac < 1:3145555/65‑1 (MQ=255) aTCGGGGTGATTTTCGGGCCTAAATCACAGCCAATca > 1:4644263/1‑37 (MQ=255) gTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCa < 1:2759639/66‑1 (MQ=255) tGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTgac < 1:5055685/58‑1 (MQ=255) gATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTga > 1:4834719/1‑56 (MQ=255) gATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGt > 1:4630877/1‑66 (MQ=255) gATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGt > 1:4652180/1‑66 (MQ=255) aTTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCg < 1:2556719/70‑1 (MQ=255) aTTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCg < 1:3932247/70‑1 (MQ=255) ttttCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCa < 1:2145888/62‑1 (MQ=255) tttCGGGCCTAAATCACAGCCAATCACATTGGCATAAATc > 1:1054278/1‑40 (MQ=255) gCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGt < 1:2840378/71‑1 (MQ=255) cTAAATCACAGCTAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTc > 1:2675986/1‑70 (MQ=255) cTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTa > 1:2370807/1‑62 (MQ=255) cTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTa > 1:5079288/1‑62 (MQ=255) tAAATCACAGCTAATCACATTGGCATAAATCATTGCCTCTTTGacgacg < 1:2926018/49‑1 (MQ=255) tAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCttt < 1:1110736/42‑1 (MQ=255) tAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCttt < 1:2545977/42‑1 (MQ=255) tAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGc < 1:2217961/57‑1 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCct > 1:483440/1‑36 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTgac < 1:2218569/43‑1 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCg < 1:4618802/57‑1 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATc < 1:5142340/71‑1 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATc < 1:1796485/71‑1 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATc < 1:1893562/71‑1 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATc < 1:2182661/71‑1 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATc < 1:2213382/71‑1 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATc < 1:1044849/71‑1 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATc < 1:2447722/71‑1 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATc < 1:3723059/71‑1 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATc < 1:4354594/71‑1 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATc > 1:432248/1‑71 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATc > 1:3350177/1‑71 (MQ=255) aTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTga > 1:3209477/1‑41 (MQ=255) aTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTga > 1:3215508/1‑41 (MQ=255) aTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGcc > 1:2167071/1‑55 (MQ=255) aTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATc < 1:4407008/70‑1 (MQ=255) tCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCg < 1:2625776/55‑1 (MQ=255) tCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCg < 1:2888038/55‑1 (MQ=255) tCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCg < 1:3038604/55‑1 (MQ=255) tCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa < 1:516167/71‑1 (MQ=255) cAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgc < 1:3313904/70‑1 (MQ=255) | GCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGC > W3110S.gb/2770941‑2771080 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |