| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,771,105 | G→A | 40.5% | N79N (AAC→AAT) | ypjL ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,771,105 | 0 | G | A | 40.5% | 53.2 / 86.0 | 74 | N79N (AAC→AAT) | ypjL | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (21/23); new base A (14/16); total (35/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
CCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTC > W3110S.gb/2771035‑2771173 | ccTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTg < 1:2090699/71‑1 (MQ=255) ctctTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGt < 1:3002205/71‑1 (MQ=255) tGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTtc < 1:2497875/68‑1 (MQ=255) tGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTtc < 1:1628547/68‑1 (MQ=255) cgacgCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACt < 1:399211/71‑1 (MQ=255) gCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACtgat > 1:438848/1‑70 (MQ=255) gCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACtgat > 1:4166184/1‑70 (MQ=255) ccAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTATtcat < 1:2217661/62‑1 (MQ=255) ccAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACtgatga < 1:3464561/71‑1 (MQ=255) aGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACt < 1:1579971/64‑1 (MQ=255) tCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTATtcat < 1:1228548/58‑1 (MQ=255) cGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACTGATgagaga > 1:3497944/1‑70 (MQ=255) cGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACTGATgagaga > 1:3497942/1‑70 (MQ=255) gCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGaaa > 1:253564/1‑71 (MQ=255) gCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGaaa > 1:3701873/1‑71 (MQ=255) ccGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAAt > 1:4373814/1‑71 (MQ=255) gTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGc < 1:522743/71‑1 (MQ=255) cTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGt > 1:2772654/1‑71 (MQ=255) cTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACt < 1:4061904/54‑1 (MQ=255) cTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACt < 1:2377719/54‑1 (MQ=255) cTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACt < 1:119961/54‑1 (MQ=255) cTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACt < 1:4879429/54‑1 (MQ=255) cTCCCGTCAATCGACAGCGCA‑CAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTc < 1:2640012/71‑1 (MQ=255) cccGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGaa > 1:3929727/1‑62 (MQ=255) ccGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCa < 1:3668276/70‑1 (MQ=255) ccGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCa < 1:5089026/70‑1 (MQ=255) cGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCaag < 1:24196/71‑1 (MQ=255) cGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCaag < 1:274658/71‑1 (MQ=255) cAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACtgatga > 1:4455891/1‑52 (MQ=255) cAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACtgatga > 1:3433357/1‑52 (MQ=255) cAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACtgatga > 1:866418/1‑52 (MQ=255) cAATCGACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCaagaag > 1:3824161/1‑71 (MQ=255) tCGACAGCGCACCAATCAGCACCGTCGGCATATTGtt < 1:3716232/37‑1 (MQ=255) tCGACAGCGCACCAATCAGCACCGTCGGCATATTGtt < 1:2938009/37‑1 (MQ=255) gcgcACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg < 1:1417825/71‑1 (MQ=255) cgcACCAATCAGCACCGTCGGCATATTATTCATCACTGATgaga > 1:2846968/1‑44 (MQ=255) aCCAATCAGCACCGTCGGCATATTGTTCATCACTGATgagaga < 1:1344186/43‑1 (MQ=255) cAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGCACGTc > 1:245458/1‑71 (MQ=255) aaTCAGCACCGTCGGCATATTGTTCATCACTGATgagaga > 1:3163352/1‑40 (MQ=255) aTCAGCACCGTCGGCATATTGTTCATCACTGATgagaga > 1:1222380/1‑39 (MQ=255) aTCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGc > 1:4194042/1‑71 (MQ=255) tCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGc < 1:3368452/54‑1 (MQ=255) tCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGc < 1:1409992/54‑1 (MQ=255) tCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCt > 1:2929147/1‑71 (MQ=255) cAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTg > 1:3577284/1‑71 (MQ=255) aGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaa > 1:3356088/1‑38 (MQ=255) aGCACCGTCGGCATATTATTCATCACTGATGAGAGaaa > 1:3314914/1‑38 (MQ=255) aGCACCGTCGGCATATTATTCATCACTGATGAGAGaaa > 1:732278/1‑38 (MQ=255) aGCACCGTCGGCATATTATTCATCACTGATGAGAGaaa > 1:1405469/1‑38 (MQ=255) aGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCaagaa > 1:3981796/1‑50 (MQ=255) gCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGcc > 1:3154163/1‑71 (MQ=255) gCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGc > 1:2595373/1‑70 (MQ=255) cACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGccc < 1:4799117/71‑1 (MQ=255) aCCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGccc < 1:2033494/70‑1 (MQ=255) aCCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGccc < 1:2462938/70‑1 (MQ=255) ccGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCa > 1:4573591/1‑70 (MQ=255) cGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGc < 1:3166498/66‑1 (MQ=255) tCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTc < 1:3537837/59‑1 (MQ=255) tCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTc < 1:3543949/59‑1 (MQ=255) tCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTc < 1:696045/59‑1 (MQ=255) tCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTg > 1:1139578/1‑63 (MQ=255) tCGGCATATTATTCATCACTGATGAGAGAAATGCCTTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAAc < 1:1052405/71‑1 (MQ=255) tCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAAc < 1:1999646/71‑1 (MQ=255) tCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAAc > 1:1028586/1‑71 (MQ=255) ggCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAAccc > 1:3850929/1‑71 (MQ=255) gCATATTATTCATCACTGATGAGAGAAATGCCGTCa < 1:2626484/36‑1 (MQ=255) gCATATTATTCATCACTGATGAGAGAAATGCCGTCa < 1:4795969/36‑1 (MQ=255) gCATATTATTCATCACTGATGAGAGAAATGCCGTCa < 1:2388668/36‑1 (MQ=255) gCATATTATTCATCACTGATGAGAGAAATGCCGTCa < 1:3207913/36‑1 (MQ=255) atatTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCtt < 1:1498211/71‑1 (MQ=255) atatTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCgg > 1:1095447/1‑43 (MQ=255) atatTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCtt > 1:243528/1‑71 (MQ=255) ttGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCAt < 1:272183/60‑1 (MQ=255) ttGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTc > 1:1295631/1‑71 (MQ=255) ttGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTc > 1:1301549/1‑71 (MQ=255) | CCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTC > W3110S.gb/2771035‑2771173 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |