| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,809,581 | G→A | 53.6% | G52G (GGG→GGA) | mprA → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,809,581 | 0 | G | A | 53.6% | 14.0 / 165.5 | 112 | G52G (GGG→GGA) | mprA | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (23/29); new base A (33/27); total (56/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01 | |||||||||||
Only 100 of 116 total aligned reads displayed.
ACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTG > W3110S.gb/2809513‑2809651 | aCTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGa < 1:5061340/70‑1 (MQ=255) cTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGAtt < 1:672796/71‑1 (MQ=255) cTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGAt > 1:1027788/1‑70 (MQ=255) tCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAAc > 1:2463563/1‑70 (MQ=255) cTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAAc > 1:2100794/1‑69 (MQ=255) tgcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACgag < 1:4601147/69‑1 (MQ=255) tgcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACga < 1:4445723/68‑1 (MQ=255) atgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgtt < 1:697765/71‑1 (MQ=255) gcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACgttgt > 1:1172825/1‑71 (MQ=255) cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgtt > 1:878085/1‑71 (MQ=255) cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACgttgtt < 1:2995038/71‑1 (MQ=255) cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACgttgtt < 1:2873791/71‑1 (MQ=255) acatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGttt < 1:666736/71‑1 (MQ=255) acatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGttt < 1:3342328/71‑1 (MQ=255) atgcaAAGCAAGCTGCTGGAGAACCGTAATAAAATGCTGAAGGCTCAGGGAATTAACga < 1:130007/59‑1 (MQ=255) atgcaAAGCAAGATGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTAt > 1:4414454/1‑71 (MQ=255) tgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTTAAGGCTCAGGGAATTAACga < 1:4948755/58‑1 (MQ=255) tgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAAtt < 1:2604523/53‑1 (MQ=255) gcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATg > 1:3564173/1‑70 (MQ=255) caAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGc > 1:2394093/1‑71 (MQ=255) caAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATg > 1:2917712/1‑69 (MQ=255) caAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGc > 1:4471591/1‑71 (MQ=255) caAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGc > 1:4866297/1‑71 (MQ=255) caAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGc > 1:2632434/1‑71 (MQ=255) caAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGc > 1:13921/1‑71 (MQ=255) caAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGc > 1:4675994/1‑71 (MQ=255) caAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGc > 1:4836808/1‑71 (MQ=255) aaaGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTAt > 1:2279470/1‑67 (MQ=255) aagcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGttt < 1:2449609/64‑1 (MQ=255) aagcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGt < 1:1235896/71‑1 (MQ=255) aagcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTAt < 1:860479/66‑1 (MQ=255) agcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTaa > 1:4357604/1‑50 (MQ=255) agcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAAtt < 1:4328979/48‑1 (MQ=255) agcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAAtt < 1:2605038/48‑1 (MQ=255) gcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATgg > 1:470354/1‑66 (MQ=255) aagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTaa > 1:5103371/1‑47 (MQ=255) aagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAt < 1:4503515/71‑1 (MQ=255) aagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGAt < 1:3558901/71‑1 (MQ=255) agcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGAtt < 1:831005/71‑1 (MQ=255) gctgctGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGttt < 1:2480898/58‑1 (MQ=255) gctgctGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGtt < 1:204150/66‑1 (MQ=255) gctgctGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTa < 1:1895111/71‑1 (MQ=255) ctgctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACgag < 1:725883/48‑1 (MQ=255) tgctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACg > 1:4566920/1‑71 (MQ=255) tgctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACg > 1:1992960/1‑71 (MQ=255) ggAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTa > 1:2235424/1‑53 (MQ=255) gagaACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgtt < 1:5146035/50‑1 (MQ=255) agaACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAg > 1:661789/1‑71 (MQ=255) gaACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACg > 1:5078965/1‑43 (MQ=255) gaACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg > 1:2677486/1‑67 (MQ=255) aaCCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtc > 1:5144997/1‑71 (MQ=255) ccGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtct > 1:2694208/1‑70 (MQ=255) cGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTgg < 1:1145400/64‑1 (MQ=255) cGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTgg < 1:1721032/64‑1 (MQ=255) cGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg > 1:4898771/1‑63 (MQ=255) gCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAg > 1:2403032/1‑71 (MQ=255) gCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAg > 1:4499095/1‑71 (MQ=255) cAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgtt < 1:382249/42‑1 (MQ=255) cAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAt < 1:324521/54‑1 (MQ=255) cAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTa > 1:2610905/1‑44 (MQ=255) aTAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg > 1:3671120/1‑59 (MQ=255) aTAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg > 1:2490410/1‑59 (MQ=255) aTAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGa > 1:4579036/1‑70 (MQ=255) tAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaaa < 1:63651/71‑1 (MQ=255) tAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaaa < 1:301269/71‑1 (MQ=255) tAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGttt < 1:1718336/40‑1 (MQ=255) tAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGttt < 1:3719493/40‑1 (MQ=255) tAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAg > 1:2684511/1‑61 (MQ=255) tAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAg > 1:4651474/1‑61 (MQ=255) tAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGa < 1:1868450/69‑1 (MQ=255) aaaaTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg < 1:2237595/57‑1 (MQ=255) aaaaTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg < 1:4132305/57‑1 (MQ=255) aaaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTa > 1:3546084/1‑40 (MQ=255) aaaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTa > 1:1370848/1‑40 (MQ=255) aaaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTa > 1:603437/1‑40 (MQ=255) aaaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTa > 1:2806609/1‑40 (MQ=255) aaaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaaaa > 1:2745307/1‑71 (MQ=255) aaaTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTa > 1:4991205/1‑39 (MQ=255) aaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAAc > 1:4895245/1‑71 (MQ=255) aTGCTGAAGGCTCAGTGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaaaa > 1:2977991/1‑68 (MQ=255) aTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaaaa > 1:3245554/1‑68 (MQ=255) aTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACca < 1:3740691/71‑1 (MQ=255) cTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAg < 1:2893421/71‑1 (MQ=255) cTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAg < 1:3447212/71‑1 (MQ=255) tGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCa < 1:276880/58‑1 (MQ=255) tGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAg < 1:1885782/70‑1 (MQ=255) aaGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAg > 1:2025607/1‑68 (MQ=255) aaGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTAt > 1:3042496/1‑71 (MQ=255) aaGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAgg > 1:3599617/1‑58 (MQ=255) aaGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTa < 1:5087749/70‑1 (MQ=255) aaGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTAt > 1:380083/1‑71 (MQ=255) aGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTAtt < 1:948337/71‑1 (MQ=255) ggCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTc < 1:3549331/71‑1 (MQ=255) gCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCa < 1:4649322/71‑1 (MQ=255) gCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCa < 1:4558700/71‑1 (MQ=255) gCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCa < 1:843004/71‑1 (MQ=255) gATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaaaa > 1:3194617/1‑51 (MQ=255) gATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaaaa > 1:2733481/1‑51 (MQ=255) gATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAGCCACAGTATTCAGCCTTCt > 1:3657431/1‑70 (MQ=255) gATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTg < 1:4357376/71‑1 (MQ=255) | ACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTG > W3110S.gb/2809513‑2809651 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |