| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,820,171 | G→A | 10.0% | A166V (GCA→GTA) | alaS ← | alanyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,820,171 | 0 | G | A | 10.0% | 151.4 / 5.4 | 50 | A166V (GCA→GTA) | alaS | alanyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base G (29/16); new base A (2/3); total (31/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCC > W3110S.gb/2820104‑2820239 | aTCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCg < 1:4150943/70‑1 (MQ=255)aTCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCg > 1:481108/1‑70 (MQ=255) cGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTAt > 1:2606611/1‑71 (MQ=255) cGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTAt > 1:123875/1‑71 (MQ=255) aggTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCa > 1:334842/2‑65 (MQ=255) gTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGtt < 1:3398840/71‑1 (MQ=255) gTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGt > 1:3363297/1‑70 (MQ=255) cGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGc > 1:1048712/1‑71 (MQ=255) cGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGc > 1:2053922/1‑71 (MQ=255) cGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGc > 1:4597038/1‑71 (MQ=255) cGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGc > 1:1357424/1‑71 (MQ=255) gggCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGcc > 1:2345711/1‑71 (MQ=255) ggCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCg < 1:4287274/71‑1 (MQ=255) ggCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCg < 1:2727563/71‑1 (MQ=255) ccGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGAt > 1:1757564/1‑71 (MQ=255) cACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGc < 1:3740950/64‑1 (MQ=255) cGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCg < 1:2825138/40‑1 (MQ=255) cGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGt > 1:1606833/1‑56 (MQ=255) cGGAACAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGt > 1:1287588/1‑56 (MQ=255) gtgtCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACg > 1:2768045/1‑71 (MQ=255) gtgtCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACg > 1:3213355/1‑71 (MQ=255) tgtCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGcc < 1:3400683/55‑1 (MQ=255) tCACCCATCTGCCAGAAGTTGTCAGATACGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGtt > 1:5136916/1‑70 (MQ=255) tCACCCATCTGCCAGAAGTTGTCAGATACGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTc > 1:192371/1‑71 (MQ=255) aCCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTcg < 1:3677315/70‑1 (MQ=255) aCCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTcg < 1:1159228/70‑1 (MQ=255) cccATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGtt < 1:1792688/44‑1 (MQ=255) ccATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTg < 1:4261450/41‑1 (MQ=255) cATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTcgcgcg > 1:910712/1‑71 (MQ=255) aTCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGt < 1:4958365/40‑1 (MQ=255) tCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGaataa < 1:5141420/57‑1 (MQ=255) tCTGCCAGAAGTTGTCAGATACGTATGGCGCACCTTTGtt < 1:1398721/40‑1 (MQ=255) tGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGaata > 1:3642126/1‑54 (MQ=255) tGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGaata > 1:3770356/1‑54 (MQ=255) tGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGaata > 1:751935/1‑54 (MQ=255) ccAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCggg < 1:4020680/67‑1 (MQ=255) ccAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCggg < 1:227241/67‑1 (MQ=255) gAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGaa > 1:1453256/1‑47 (MQ=255) gAAGTTGTCAGATGCGTATGGCGCACATTTGTTATCGCCGATGCGaa > 1:5135396/1‑47 (MQ=255) aaGTTGTCAGATACGTATGGCGCACCTTTGTTATCGCCGATGCGaataa < 1:2038085/49‑1 (MQ=255) aaGTTGTCAGATACGTATGGCGCACCTTTGTTATCGCCGATGCGaataa < 1:2031353/49‑1 (MQ=255) aGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTcgcgcg > 1:1630215/1‑60 (MQ=255) gTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTagc > 1:1066526/1‑68 (MQ=255) gTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTcg > 1:5014403/1‑52 (MQ=255) gTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTcg > 1:4021881/1‑52 (MQ=255) gTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTcg > 1:2609853/1‑52 (MQ=255) cAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTActtctt < 1:482178/69‑1 (MQ=255) aTGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTcc > 1:4070480/1‑71 (MQ=255) aTGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTcc > 1:586973/1‑71 (MQ=255) aTGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTcc > 1:590301/1‑71 (MQ=255) | ATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCCCTACTTCTTTTTCC > W3110S.gb/2820104‑2820239 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |