| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,841,531 | G→A | 40.4% | R57* (CGA→TGA) | hycI ← | protease involved in processing C‑terminal end of HycE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,841,531 | 0 | G | A | 40.4% | 36.6 / 59.2 | 52 | R57* (CGA→TGA) | hycI | protease involved in processing C‑terminal end of HycE |
| Reads supporting (aligned to +/- strand): ref base G (7/24); new base A (11/10); total (18/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.87e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
TCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCAC > W3110S.gb/2841461‑2841594 | tcatcCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCg > 1:64323/1‑71 (MQ=255) catcCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCgt > 1:1631469/1‑71 (MQ=255) atcCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCgt < 1:4471854/70‑1 (MQ=255) cGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCg < 1:4763366/71‑1 (MQ=255) cGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCATGTc < 1:3340050/70‑1 (MQ=255) cGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCATGTCGGgcg > 1:3389535/1‑70 (MQ=255) gatgatGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGgcgc < 1:2639061/70‑1 (MQ=255) gatgatGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGgcgc < 1:2295953/70‑1 (MQ=255) tgatgCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGt < 1:1744159/71‑1 (MQ=255) tgCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCa < 1:2023848/71‑1 (MQ=255) gCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCATGTCGGGCGCAGTTCa < 1:1174454/70‑1 (MQ=255) cGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCGGTCATGTCGGGCGCAGTTCAc < 1:1679918/70‑1 (MQ=255) aTCTCGGCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTc < 1:3039740/54‑1 (MQ=255) aTCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTc < 1:4408288/54‑1 (MQ=255) aTCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTc < 1:2133700/54‑1 (MQ=255) tctcGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCATGTCGGGCGCAGTTCACGGa > 1:486467/1‑69 (MQ=255) tctcGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCATGTCGGGCGCAGTTCACGGa > 1:1602685/1‑69 (MQ=255) ctcGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTc < 1:1310145/52‑1 (MQ=255) tcGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCATGTCGGGCGCAGtac > 1:1644613/1‑60 (MQ=255) gCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGc < 1:4096928/69‑1 (MQ=255) ccGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCATGTCGGGCGCAGTTCACGGATAGcgac < 1:3642732/71‑1 (MQ=255) ccGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCATGTCGGGCGCAGTTCACGGATAGcgac < 1:2505502/71‑1 (MQ=255) ggTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGgcgc < 1:1304958/50‑1 (MQ=255) tttAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCgt < 1:113255/39‑1 (MQ=255) tttAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCgt < 1:2017202/39‑1 (MQ=255) tttAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGcgacg < 1:1954087/66‑1 (MQ=255) tAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCg < 1:797457/70‑1 (MQ=255) tAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCATGTCGGGCGCAGTTCACGGATAGCGACGATGTCGt > 1:2385564/1‑71 (MQ=255) cccATATCCGTGGCGTCGACAATCAGCAGTCATGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTc > 1:5033050/1‑71 (MQ=255) atatCCGTGGCGTCGACAATCAGCAGTCATGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTcc < 1:3537108/69‑1 (MQ=255) tatCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCa > 1:904175/1‑44 (MQ=255) tCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTgc > 1:3604345/1‑71 (MQ=255) tCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTgc > 1:4818536/1‑71 (MQ=255) tCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTg > 1:4135273/1‑70 (MQ=255) tCCGTGGCGTCGACAATCAGCAGTCATGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTcc > 1:3411626/1‑66 (MQ=255) tCCGTGGCGTCGACAATCAGCAGTCATGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTcc > 1:1204658/1‑66 (MQ=255) tCCGTGGCGTCGACAATCAGCAGTCATGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTg < 1:846568/70‑1 (MQ=255) cGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTgcgc < 1:1552481/71‑1 (MQ=255) cGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTgcgc < 1:1230839/71‑1 (MQ=255) cGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATCTCGTTTTCCGGTgcgc < 1:1887421/71‑1 (MQ=255) cGTGGCGTCGACAATCAGCAGTCATGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTgcgc < 1:2646089/71‑1 (MQ=255) tGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAg < 1:353720/45‑1 (MQ=255) tGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAg < 1:4092136/45‑1 (MQ=255) tGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCTCTa < 1:3746088/71‑1 (MQ=255) tGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTa < 1:459481/71‑1 (MQ=255) gCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTAc > 1:4720766/1‑70 (MQ=255) gTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACg < 1:2296331/36‑1 (MQ=255) tCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTAccgc > 1:3690384/1‑70 (MQ=255) tCGACAATCAGCAGTCATGTCGGGCGCAGTTCACGGATAGcgac < 1:3098505/44‑1 (MQ=255) gACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGa > 1:2780198/1‑35 (MQ=255) aCAATCAGCAGTCATGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTcc > 1:4216922/1‑54 (MQ=255) aCAATCAGCAGTCATGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCgg > 1:1295283/1‑56 (MQ=255) aCAATCAGCAGTCATGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCgg > 1:4092195/1‑56 (MQ=255) cAATCAGCAGTCATGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCa < 1:367736/71‑1 (MQ=255) aaTCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGcgac < 1:4944953/39‑1 (MQ=255) tCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATc < 1:3877607/71‑1 (MQ=255) agcagTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATcac < 1:3290157/71‑1 (MQ=255) | TCATCCGGGTCGATGATGCGGATCTCGCCGGGGTTTAGCCCCATATCCGTGGCGTCGACAATCAGCAGTCGTGTCGGGCGCAGTTCACGGATAGCGACGATGTCGTTTTCCGGTGCGCTACCGCCGTCAATCAC > W3110S.gb/2841461‑2841594 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |