| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,863,059 | G→A | 41.4% | L56L (CTG→CTA) | ygbM → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,863,059 | 0 | G | A | 41.4% | 42.1 / 84.0 | 70 | L56L (CTG→CTA) | ygbM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (18/23); new base A (15/14); total (33/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01 | |||||||||||
TTTCTGTTTCCCTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGT > W3110S.gb/2862991‑2863128 | tttCTGTTTCCCTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgc > 1:4180040/1‑71 (MQ=255)tttCTGTTTCCCTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgc > 1:4245543/1‑71 (MQ=255) tCTGTTTCCCTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGgcg < 1:136952/70‑1 (MQ=255) tCTGTTTCCCTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgcgc > 1:3394858/1‑71 (MQ=255) tCCCTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTc < 1:380801/70‑1 (MQ=255) cccTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTc < 1:2743352/69‑1 (MQ=255) ccTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCaa < 1:5133839/70‑1 (MQ=255) ccTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCaa < 1:3183549/70‑1 (MQ=255) tataACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTc < 1:1259987/66‑1 (MQ=255) tataACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTc < 1:204583/66‑1 (MQ=255) tataACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTc < 1:185903/66‑1 (MQ=255) tataACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCaa < 1:3399418/68‑1 (MQ=255) ataACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTc < 1:4618062/65‑1 (MQ=255) aaCTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTc < 1:179444/63‑1 (MQ=255) aaCTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTCACACTGGCGCTGTTc > 1:2304634/1‑63 (MQ=255) actCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTc < 1:3205310/69‑1 (MQ=255) ctCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTcc < 1:3121564/69‑1 (MQ=255) ccACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATa > 1:4625065/1‑60 (MQ=255) cACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCaa > 1:2995243/1‑57 (MQ=255) aCCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGgaga < 1:4619192/71‑1 (MQ=255) ccTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAgcg < 1:1185014/46‑1 (MQ=255) cTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCaa > 1:2390381/1‑53 (MQ=255) cTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGgaga > 1:1328454/1‑68 (MQ=255) tGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGga < 1:3425553/65‑1 (MQ=255) tGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGga < 1:2780848/65‑1 (MQ=255) tGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGatat > 1:865104/1‑70 (MQ=255) gCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCaa > 1:888169/1‑51 (MQ=255) gCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGatat > 1:534145/1‑69 (MQ=255) cAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATAtt < 1:4756284/69‑1 (MQ=255) cAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATAtt < 1:4342282/69‑1 (MQ=255) cAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATAtt < 1:2849795/69‑1 (MQ=255) aaTCCAAAAGCAACTGGAGCAAAACCATCTGACACTGgcgc > 1:1862442/1‑41 (MQ=255) aaTCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATg > 1:1762639/1‑71 (MQ=255) aTCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCaa > 1:1290451/1‑47 (MQ=255) aTCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCaa > 1:1408632/1‑47 (MQ=255) aTCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATAtt < 1:2894646/66‑1 (MQ=255) aTCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGc < 1:3514658/71‑1 (MQ=255) aTCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGc < 1:2497777/71‑1 (MQ=255) ccAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTa > 1:3566810/1‑65 (MQ=255) ccAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTc > 1:4581090/1‑43 (MQ=255) ccAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTc > 1:1793716/1‑43 (MQ=255) ccAAAAGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTc > 1:4268914/1‑43 (MQ=255) aaaGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCgggg > 1:3440018/1‑70 (MQ=255) aaaGCAACTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCgggg > 1:2504661/1‑70 (MQ=255) aaGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTcc > 1:3074303/1‑50 (MQ=255) aaGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAAt > 1:2311985/1‑63 (MQ=255) aaGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGa < 1:995742/71‑1 (MQ=255) aaCTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATAc > 1:2519705/1‑40 (MQ=255) aaCTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATg > 1:3206316/1‑60 (MQ=255) aaCTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATg > 1:3209930/1‑60 (MQ=255) aCTGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGga < 1:4348572/49‑1 (MQ=255) tGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATAtt < 1:2334138/53‑1 (MQ=255) tGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATAtt < 1:3946298/53‑1 (MQ=255) tGGAGCAAAACCATCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGa > 1:2233540/1‑71 (MQ=255) gAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGAt > 1:1062962/1‑70 (MQ=255) aCCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGaa > 1:1391312/1‑56 (MQ=255) tCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGatat < 1:357970/39‑1 (MQ=255) tCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATAtt < 1:369641/40‑1 (MQ=255) tCTGACACTAGCGCTGTTCAATACCGCTCCCGGAGATAtt < 1:1369071/40‑1 (MQ=255) gACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATgg > 1:61131/1‑52 (MQ=255) gACACTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATgg > 1:1958296/1‑52 (MQ=255) acacTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGaa > 1:4140171/1‑48 (MQ=255) acacTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTcc < 1:1890035/68‑1 (MQ=255) acacTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTcc < 1:51604/68‑1 (MQ=255) acacTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCg < 1:4591739/70‑1 (MQ=255) acacTAGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCg < 1:4277759/70‑1 (MQ=255) acTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGAc < 1:2552492/71‑1 (MQ=255) cTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCggggg < 1:4212427/43‑1 (MQ=255) cTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCggggg < 1:3929410/43‑1 (MQ=255) tGGCGCTGTTCAATACCGCTCACGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGt > 1:4046745/1‑71 (MQ=255) | TTTCTGTTTCCCTATAACTACTCCACCCTGCAAATCCAAAAGCAACTGGAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGT > W3110S.gb/2862991‑2863128 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |