| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,866,110 | T→A | 100% | intergenic (‑20/+160) | rpoS ← / ← nlpD | RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,866,110 | 0 | T | A | 100.0% | 207.0 / NA | 68 | intergenic (‑20/+160) | rpoS/nlpD | RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (33/35); total (33/35) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTA > W3110S.gb/2866043‑2866176 | cAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt > 1:373078/1‑71 (MQ=255)cAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt > 1:3035283/1‑71 (MQ=255) aaCACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTc < 1:4333817/71‑1 (MQ=255) aaCACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTc < 1:3907622/71‑1 (MQ=255) acacGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctac > 1:1708262/1‑71 (MQ=255) acacGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctac > 1:2103673/1‑71 (MQ=255) acGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctacta < 1:4602595/71‑1 (MQ=255) tgtgGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCt > 1:3191562/1‑71 (MQ=255) tgtgGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCt < 1:2848520/71‑1 (MQ=255) gCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCtttt < 1:5054633/70‑1 (MQ=255) gCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCtttt < 1:3051732/70‑1 (MQ=255) cTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTc < 1:4598409/51‑1 (MQ=255) cTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTc < 1:3679487/51‑1 (MQ=255) cTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaa < 1:969795/71‑1 (MQ=255) cGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaaa < 1:5087198/70‑1 (MQ=255) tAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCt > 1:193237/1‑71 (MQ=255) aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctact < 1:3844100/47‑1 (MQ=255) aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTaa < 1:2862782/49‑1 (MQ=255) aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTc < 1:3825429/70‑1 (MQ=255) aGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTaa > 1:4579653/1‑47 (MQ=255) aGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAgg > 1:3727864/1‑49 (MQ=255) aGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAgg > 1:4721558/1‑49 (MQ=255) ttCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaa < 1:1000098/60‑1 (MQ=255) tCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTcc > 1:3841060/1‑71 (MQ=255) ccTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTc > 1:1642359/1‑37 (MQ=255) ccTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTc > 1:2392161/1‑37 (MQ=255) ccTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTaa > 1:1728755/1‑43 (MQ=255) ccTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTaa > 1:875240/1‑43 (MQ=255) ccTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTaa > 1:1814880/1‑43 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGt > 1:4616516/1‑71 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGt < 1:5084403/71‑1 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGt < 1:629687/71‑1 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGt > 1:2346666/1‑71 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGt > 1:2575720/1‑71 (MQ=255) tctTCGGCCAAATCGTTATCACTGGGTTCCAATTctact < 1:4608518/39‑1 (MQ=255) tctTCGGCCAAATCGTTATCACTGGGTTCCAATTcta < 1:4747634/37‑1 (MQ=255) tctTCGGCCAAATCGTTATCACTGGGTTCCAATTc < 1:915760/35‑1 (MQ=255) tctTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCtt < 1:3461368/47‑1 (MQ=255) ttCGGCCAAATCGTTATCACTGGGTTCCAATTctac < 1:4621661/36‑1 (MQ=255) ttCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaaaa > 1:2721477/1‑56 (MQ=255) tCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAg > 1:1075466/1‑39 (MQ=255) cGGCCAAATCGTTATCACTGGGTTCCAATTctacta > 1:3371108/1‑36 (MQ=255) cGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtctc > 1:4943961/1‑70 (MQ=255) gCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCtcatc < 1:2152242/71‑1 (MQ=255) ccAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCtcatca < 1:4097911/71‑1 (MQ=255) ccAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCtcatca < 1:583993/71‑1 (MQ=255) aaaTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaa < 1:4206747/47‑1 (MQ=255) aaTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTc < 1:5100657/52‑1 (MQ=255) aaTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAAt < 1:530360/71‑1 (MQ=255) aTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaaa > 1:147212/1‑46 (MQ=255) tCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTc > 1:4437614/1‑71 (MQ=255) tCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTc > 1:2542092/1‑71 (MQ=255) tCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTc > 1:495850/1‑71 (MQ=255) tCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTc > 1:1897064/1‑71 (MQ=255) cGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCaa < 1:569208/51‑1 (MQ=255) gTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaaaa > 1:2252728/1‑44 (MQ=255) gTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaaaa > 1:3627441/1‑44 (MQ=255) gTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTcc < 1:2075914/70‑1 (MQ=255) gTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCg > 1:281332/1‑71 (MQ=255) gTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCg > 1:4322906/1‑71 (MQ=255) gTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCg > 1:3053233/1‑71 (MQ=255) ttATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGc < 1:5146787/71‑1 (MQ=255) tCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaa < 1:2989595/38‑1 (MQ=255) tCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaa < 1:1668494/38‑1 (MQ=255) aCTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCtt < 1:4265999/71‑1 (MQ=255) aCTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCtt < 1:2370144/71‑1 (MQ=255) gggTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCAt > 1:1811865/1‑71 (MQ=255) ttCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTa < 1:5105317/71‑1 (MQ=255) | CAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTA > W3110S.gb/2866043‑2866176 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |