Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,880,475 G→A 14.8% A108A (GCC→GCT)  ygcJ ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,880,4750GA14.8% 141.2 / 15.2 54A108A (GCC→GCT) ygcJhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/18);  new base A (5/3);  total (33/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

TGCTTTTGCAACCTGCTCACAGAACCAGGCTATTTCTCCCACAACCCAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAGCGCTAATGTCTTATCGATGATTTTTT  >  W3110S.gb/2880412‑2880545
                                                               |                                                                      
tGCTTTTGCAACCTGCTCACAGAACCAGGCTATTTCTCCCACAACCCAGGGAGTAACCGCATCGTCAGaaa                                                                 >  1:1133191/1‑71 (MQ=255)
tGCTTTTGCAACCTGCTCACAGAACCAGGCTATTTCTCCCACAACCCAGGGAGTAACCGCATCGGCAGaaa                                                                 >  1:695444/1‑71 (MQ=255)
tGCTTTTGCAACCTGCTCACAGAACCAGGCTATTTCTCCCACAACCCAGGGAGTAACCGCATCGGCAGaaa                                                                 >  1:3911976/1‑71 (MQ=255)
tGCTTTTGCAACCTGCTCACAGAACCAGGCTATTTCTCCCACAACCCAGGGAGTAACCGCATCGGCAGa                                                                   <  1:4297783/69‑1 (MQ=255)
     ttGCAACCTGCTCACAGAACCAGGCTATTTCTCCCACAACCCAGGGAGTAACCGCATCGGCAGAAATCtt                                                             <  1:1948274/70‑1 (MQ=255)
     ttGCAACCTGCTCACAGAACCAGGCTATTTCTCCCACAACCCAGGGAGTAACCGCATCGGCAGAAATCtt                                                             <  1:2558609/70‑1 (MQ=255)
       gCAACCTGCTCACAGAACCAGGCTATTTCTCCCACAACCCAGGGAGTAACCGCATCGGCAGAAATCtttt                                                           <  1:2621383/70‑1 (MQ=255)
         aaCCTGCTCACAGAACCAGGCTATTTCTCCCACAACCCAGGGAGTAACCGCATCGGCAGAAATCTTTTCgg                                                        <  1:2208745/71‑1 (MQ=255)
           ccTGCTCACAGAACCAGGCTATTTCTCCCACAACCCAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCt                                                      >  1:2136630/1‑71 (MQ=255)
            cTGCTCACAGAACCAGGCTATTTCTCCCACAACCCAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCtt                                                     <  1:648701/71‑1 (MQ=255)
                  acaGAACCAGGCTATTTCTCCCACAACCCAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCTtcatc                                                 <  1:3657588/69‑1 (MQ=255)
                   caGAACCAGGCTATTTCTCCCACAACCCAGGGAGTAACCGCATCAGCAGAAATCtttt                                                           >  1:2971271/1‑58 (MQ=255)
                    aGAACCAGGCTATTTCTCCCACAACCCAGGGAGTAACCGCATCGGCAGAAATCtt                                                             <  1:2135597/55‑1 (MQ=255)
                          aGGCTATTTCTCCCACAACCCAGGGTGTAACCGCATCGGCAGAAAt                                                                >  1:2161821/1‑46 (MQ=255)
                          aGGCTATTTCTCCCACAACCCAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTa                                       >  1:416539/1‑71 (MQ=255)
                            gCTATTTCTCCCACAACCCAGGGAGTAACCGCATCAGCAGAAATCTTTTCGGCTTCATCAACTGATTTAc                                      <  1:4974354/70‑1 (MQ=255)
                                tttCTCCCACAACCCAGGGAGTAACCGCATCGGCAGAAATCtt                                                             <  1:2296309/43‑1 (MQ=255)
                                tttCTCCCACAACCCAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCg                                    <  1:4357751/68‑1 (MQ=255)
                                 ttCTCCCACAACCCAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCgg                                   >  1:3404568/1‑68 (MQ=255)
                                  tctcCCACAACCCAGGGAGTAACCGCATCGGCAGAAATCtttt                                                           >  1:3052983/1‑43 (MQ=255)
                                  tctcCCACAACCCAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAAc                                              >  1:2440591/1‑56 (MQ=255)
                                  tctcCCACAACCCAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGagag                               >  1:594343/1‑71 (MQ=255)
                                  tctcCCACAACCCAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGagag                               >  1:219331/1‑71 (MQ=255)
                                   ctcCCACAACCCAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTAcc                                     >  1:4519211/1‑64 (MQ=255)
                                   ctcCCACAACCCAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTAcc                                     >  1:3362455/1‑64 (MQ=255)
                                   ctcCCACAACCCAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGagag                               >  1:4754789/1‑70 (MQ=255)
                                        acaACCCAGGGAGTAACCGCATCGGCAGAAATCtttt                                                           >  1:3473438/1‑37 (MQ=255)
                                        acaACCCAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAgcgc                         >  1:2789025/1‑71 (MQ=255)
                                        acaACCCAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAgcgc                         >  1:401778/1‑71 (MQ=255)
                                         caACCCAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAgcgc                         >  1:2354768/1‑70 (MQ=255)
                                         caACCCAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAgcgc                         >  1:857842/1‑70 (MQ=255)
                                         caACCCAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAGCGCt                        <  1:1996239/71‑1 (MQ=255)
                                         caACCCAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAGCGCt                        <  1:1414980/71‑1 (MQ=255)
                                         caACCCAGGGAGTAACCGCATCAGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAgcgc                         >  1:3951373/1‑70 (MQ=255)
                                          aaCCCAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAGCGCt                        >  1:2838562/1‑70 (MQ=255)
                                            cccAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCtt                                                     >  1:3455298/1‑39 (MQ=255)
                                             ccAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCaa                                               >  1:2719981/1‑44 (MQ=255)
                                              cAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAGCGCTAATGt                   >  1:4575684/1‑71 (MQ=255)
                                              cAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAGCGCTAATGt                   >  1:959686/1‑71 (MQ=255)
                                              cAGGGAGTAACCGCATCAGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAGCGCTAATGt                   <  1:865448/71‑1 (MQ=255)
                                                  gAGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAGCGCTAATGTCTTa               <  1:4553516/71‑1 (MQ=255)
                                                   aGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGagag                               >  1:2968157/1‑54 (MQ=255)
                                                    gTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAGCGCTaa                      >  1:795099/1‑62 (MQ=255)
                                                    gTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAGCGCTAATGTCTTATc             <  1:623084/71‑1 (MQ=255)
                                                       aCCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGgaga                                >  1:533993/1‑49 (MQ=255)
                                                       aCCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAGCGCTAATGTCTTATCgat          <  1:2117590/71‑1 (MQ=255)
                                                         cGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAGCGCTAATGTCTTATCgatga        >  1:2804556/1‑71 (MQ=255)
                                                         cGCATCAGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGagc                              <  1:201533/49‑1 (MQ=255)
                                                         cGCATCAGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAGCGCTAATGTCTTATCgatga        >  1:3411987/1‑71 (MQ=255)
                                                         cGCATCAGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAGCGCTAATGTCTTATCgatga        >  1:3837565/1‑71 (MQ=255)
                                                          gCATCAGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGagcat                            >  1:727500/1‑49 (MQ=255)
                                                           cATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAGCGCTAATGTCtt                <  1:4373346/61‑1 (MQ=255)
                                                            aTCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAGCGCTAATGTCTTATCGATGAttt     <  1:4211070/71‑1 (MQ=255)
                                                             tCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAGCGCTAATGTCTTATCGATGAtttt    <  1:3250189/71‑1 (MQ=255)
                                                               ggCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAGCGCTAATGTCTTATCGATGAtttttt  >  1:1207008/1‑71 (MQ=255)
                                                               |                                                                      
TGCTTTTGCAACCTGCTCACAGAACCAGGCTATTTCTCCCACAACCCAGGGAGTAACCGCATCGGCAGAAATCTTTTCGGCTTCATCAACTGATTTACCGGAGAGCAGCGCTAATGTCTTATCGATGATTTTTT  >  W3110S.gb/2880412‑2880545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑