Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,882,995 C→T 19.2% intergenic (‑201/+214) ygcL ← / ← ygcB hypothetical protein/conserved hypothetical protein, member of DEAD box family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,882,9950CT19.2% 170.9 / 32.4 73intergenic (‑201/+214)ygcL/ygcBhypothetical protein/conserved hypothetical protein, member of DEAD box family
Reads supporting (aligned to +/- strand):  ref base C (29/30);  new base T (5/9);  total (34/39)
Fisher's exact test for biased strand distribution p-value = 3.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01

ATTGGTTTAATACAATTAACCTATACATATATTAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACTTTTAGTTATAATAATTACCATGAATTT  >  W3110S.gb/2882926‑2883064
                                                                     |                                                                     
aTTGGTTTAATACAATTAACCTATACATATATTAAGATGTGTTGAATTGTTTAAAGACAATAatgcatgc                                                                       <  1:1735433/70‑1 (MQ=255)
   ggTTTAATACAATTAACCTATACATATATTAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGCAttt                                                                   >  1:3760287/1‑71 (MQ=255)
    gTTTAATACAATTAACCTATACATATATTAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGCATTTc                                                                  <  1:1400768/71‑1 (MQ=255)
       tAATACAATTAACCTATACATATATTAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGTATTTCaaa                                                               >  1:891764/1‑71 (MQ=255)
        aaTACAATTAACCTATACATATATTAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGCATTTCAAAt                                                              >  1:277671/1‑71 (MQ=255)
         aTACAATTAACCTATACATATATTAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGCATTTCAAAt                                                              <  1:246131/70‑1 (MQ=255)
            cAATTAACCTATACATATATTAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGCATTTCaa                                                                >  1:3311744/1‑65 (MQ=255)
            cAATTAACCTATACATATATTAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTg                                                           <  1:1558024/70‑1 (MQ=255)
              aTTAACCTATACATATATTAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGCATTTCaa                                                                <  1:2226873/63‑1 (MQ=255)
                       taCATATATTAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGTATTTCAAATCTGCAAGTTATTCGt                                               >  1:387197/1‑71 (MQ=255)
                           tatatTAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGc                                                          <  1:423500/56‑1 (MQ=255)
                           tatatTAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGc                                                          <  1:3670151/56‑1 (MQ=255)
                            atatTAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGCATTTCAAAt                                                              >  1:334373/1‑51 (MQ=255)
                            atatTAAGATGTGTTGAATTGATTAAAGACAATAATGCATGCATTTCAAAt                                                              >  1:1345164/1‑51 (MQ=255)
                             tatTAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGTATTTCAAATCTGCAAGTTATTCGTTttat                                          <  1:4741777/70‑1 (MQ=255)
                             tatTAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGt                                                      <  1:222240/58‑1 (MQ=255)
                             tatTAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTttat                                          >  1:2151591/1‑70 (MQ=255)
                             tatTAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTttat                                          >  1:2771748/1‑70 (MQ=255)
                             tatTAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTttat                                          >  1:1225290/1‑70 (MQ=255)
                             tatTAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTttat                                          >  1:4811687/1‑70 (MQ=255)
                             tatTAAGATGTGTAGAATTGTTTAAAGCCAATAATGCATGCATTTCAAATc                                                             >  1:3639777/1‑51 (MQ=255)
                              atTAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGCATTTCa                                                                 <  1:3267830/46‑1 (MQ=255)
                               ttAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGTATTTCaa                                                                <  1:2460275/46‑1 (MQ=255)
                               ttAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGTATTTCaa                                                                <  1:2488284/46‑1 (MQ=255)
                               ttAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTttatta                                        <  1:3720397/70‑1 (MQ=255)
                               ttAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTttatta                                        <  1:3820792/70‑1 (MQ=255)
                                 aaGATGTGTTGAATTGTTTAAAGACAATAATGCATGTATTTCAAAt                                                              >  1:3872380/1‑46 (MQ=255)
                                 aaGATGTGTTGAATTGTTTAAAGACAATAATGCATGTATTTCAAAt                                                              >  1:2722241/1‑46 (MQ=255)
                                 aaGATGTGTTGAATTGTTTAAAGACAATAATGCATGTATTTCAAATCTGCAAGTTATTCGTTttattatt                                      <  1:3903396/70‑1 (MQ=255)
                                  aGATGTGTTGAATTGTTTAAAGACAATAATGCATGTATTTCAAATCTGCAAGTTATTCGTTttattatta                                     <  1:2361381/70‑1 (MQ=255)
                                  aGATGTGTTGAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTttattatta                                     >  1:37919/1‑70 (MQ=255)
                                   gATGTGTTGAATTGTTTAAAGACAATAATGCATGTATTTCAAATCTGCAAGTTATTCGTTTTATTATTaaa                                   <  1:3984495/71‑1 (MQ=255)
                                   gATGTGTTGAATTGTTTAAAGACAATAATGCATGTATTTCAAATCTGCAAGTTATTCGTTTTATTATTaaa                                   <  1:2381712/71‑1 (MQ=255)
                                   gATGTGTTGAATTGTTTAAAGACAATAATGCATGTATTTCAAATCTGCAAGTTATTAGTTTTATTATTaaa                                   <  1:1429730/71‑1 (MQ=255)
                                   gATGTGTTGAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGt                                                      <  1:3237050/52‑1 (MQ=255)
                                    aTGTGTTGAATTGTTTAAAGACAATAATGCATGTATTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAg                                  <  1:3534008/71‑1 (MQ=255)
                                     tgtgTTGAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGa                                 <  1:2901237/71‑1 (MQ=255)
                                       tgtTGAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTa                                                    >  1:352082/1‑50 (MQ=255)
                                       tgtTGAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTa                                                    >  1:3166669/1‑50 (MQ=255)
                                         ttGAATTGTTTAAAGACAATAATGCATGCATTTCa                                                                 <  1:2230413/35‑1 (MQ=255)
                                         ttGAATTGTTTAAAGACAATAATGCATGCATTTCa                                                                 <  1:3079891/35‑1 (MQ=255)
                                         ttGAATTGTTTAAAGACAATAATGCATGCATTTCa                                                                 <  1:1983572/35‑1 (MQ=255)
                                         ttGAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGtttt                                            <  1:3583268/56‑1 (MQ=255)
                                         ttGAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGt                                               <  1:2168315/53‑1 (MQ=255)
                                         ttGAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGt                                               <  1:3664342/53‑1 (MQ=255)
                                         ttGAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTTTATTATTaaa                                   <  1:3113243/65‑1 (MQ=255)
                                           gAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACttt                           >  1:4811991/1‑71 (MQ=255)
                                           gAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACttt                           >  1:2908400/1‑71 (MQ=255)
                                           gAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACttt                           >  1:4805911/1‑71 (MQ=255)
                                            aaTTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACttt                           <  1:1011154/70‑1 (MQ=255)
                                            aaTTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACttt                           <  1:131058/70‑1 (MQ=255)
                                              ttGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACTTTTAg                        >  1:1794845/1‑71 (MQ=255)
                                              ttGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACTTTTAg                        >  1:4355045/1‑71 (MQ=255)
                                                gTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACTTTTAGt                       >  1:4765149/1‑70 (MQ=255)
                                                  ttAAAGACAATAATGCATGCATTTCAAATCTGCAAg                                                       <  1:2029201/36‑1 (MQ=255)
                                                  ttAAAGACAATAATGCATGCATTTCAAATCTGCAAg                                                       <  1:1033218/36‑1 (MQ=255)
                                                    aaaGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTTTATTATTaaa                                   >  1:4884719/1‑54 (MQ=255)
                                                     aaGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACTTTTAGTTataat                 <  1:3202650/71‑1 (MQ=255)
                                                        aCAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACTTTTAGTTATaataat              <  1:635510/71‑1 (MQ=255)
                                                        aCAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACTTTTAGTTATaataat              <  1:5078517/71‑1 (MQ=255)
                                                         cAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTtta                                           >  1:4658930/1‑41 (MQ=255)
                                                          aataatGCATGCATTTCAAATCTGCAAGTTATTCGTTttat                                          <  1:3456850/41‑1 (MQ=255)
                                                          aataatGCATGCATTTCAAATCTGCAAGTTATTCGTTttat                                          <  1:227157/41‑1 (MQ=255)
                                                          aataatGCATGCATTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACTTTTAGTTATAATAATTa            >  1:1415555/1‑71 (MQ=255)
                                                           ataatGCATGCATTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACTTTTAGTTATAATAATTAc           <  1:2943397/71‑1 (MQ=255)
                                                           ataatGCATGCATTTCAAATCTGCAAGTTATTCGTTTATTATTAAAGAAACTTTTAGTTATAATAATTAc           <  1:3909845/70‑1 (MQ=255)
                                                            taatGCATGCATTTCAAATCTGCAAGTTATTCGTTttatta                                        >  1:2783522/1‑41 (MQ=255)
                                                            taatGCATGCATTTCAAATCTGCAAGTTATTCGTTttatta                                        >  1:3208067/1‑41 (MQ=255)
                                                            taatGCATGCATTTCAAATCTGCAAGTTATTCGTTttatta                                        >  1:2423634/1‑41 (MQ=255)
                                                             aatGCATGTATTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACTTTTAGTTATAATAATTACCa         >  1:4458960/1‑71 (MQ=255)
                                                             aatGCATGCATTTCAAATCTGCAAGTTATTCGTTttat                                          >  1:1540016/1‑38 (MQ=255)
                                                             aatGCATGCATTTCAAATCTGCAAGTTATTCGTTttat                                          >  1:5082466/1‑38 (MQ=255)
                                                             aatGCATGCATTTCAAATCTGCAAGTTATTCGTTttat                                          >  1:2296244/1‑38 (MQ=255)
                                                             aatGCATGCATTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACTTTTAGTTataata                >  1:2682278/1‑64 (MQ=255)
                                                              atgcatgcATTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACTTTTAGTTATAATAATTACCa         >  1:4208969/1‑70 (MQ=255)
                                                                 catgcatTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACTTTTAGTTAtaataa               >  1:4233838/1‑61 (MQ=255)
                                                                 catgcatTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACTTTTAGTTAtaataa               >  1:2273633/1‑61 (MQ=255)
                                                                    gcatTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACTTTTAGTTATAATAATTACCATGAAttt  >  1:1684693/1‑71 (MQ=255)
                                                                     catTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACTTTTAGTTATAATAATTACCATGAAttt  >  1:3966461/1‑70 (MQ=255)
                                                                     catTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACTTTTAGTTATAATAATTACCATGAAttt  <  1:1919724/70‑1 (MQ=255)
                                                                     catTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACTTTTAGTTATAATAATTACCATGAAttt  >  1:3621826/1‑70 (MQ=255)
                                                                     |                                                                     
ATTGGTTTAATACAATTAACCTATACATATATTAAGATGTGTTGAATTGTTTAAAGACAATAATGCATGCATTTCAAATCTGCAAGTTATTCGTTTTATTATTAAAGAAACTTTTAGTTATAATAATTACCATGAATTT  >  W3110S.gb/2882926‑2883064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: