| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,902,804 | C→A | 64.3% | intergenic (+774/+599) | ygcE → / ← ygcF | predicted kinase/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,902,804 | 0 | C | A | 64.3% | 2.7 / 34.1 | 28 | intergenic (+774/+599) | ygcE/ygcF | predicted kinase/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (6/4); new base A (11/7); total (17/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGC > W3110S.gb/2902742‑2902869 | gCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCgg > 1:2548044/1‑71 (MQ=255) gcgcGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGATGGCGCgggg < 1:4515203/69‑1 (MQ=255) gcgcGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGATGGCGCgggg < 1:565064/69‑1 (MQ=255) gcgcGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGATGGCGCgggg < 1:571088/69‑1 (MQ=255) cgGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAacac < 1:2292442/71‑1 (MQ=255) aaCACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACgg > 1:1846478/1‑68 (MQ=255) aaCACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGgcg < 1:4715414/71‑1 (MQ=255) acacCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGATGGCGCGGGGAACACGGgcgc < 1:1583386/71‑1 (MQ=255) ccTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACgg > 1:3317915/1‑64 (MQ=255) ttttCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGaa > 1:2053455/1‑70 (MQ=255) ttttCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGATGGCGCGGGGaa > 1:3852853/1‑55 (MQ=255) tttCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGATGGCGCGGGGAACACggg > 1:4898218/1‑60 (MQ=255) ttCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGATGGCGCGGGGAACACgg > 1:2506830/1‑58 (MQ=255) ttCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGATGGCGCGGGGAACACGGGCGCACGGAATa > 1:988065/1‑70 (MQ=255) cGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGATGGCGCggg < 1:1422832/48‑1 (MQ=255) aaTCTATGGACTATTGCTATTCGGTTTATCCCCGATGGCGCGGGGAACACGGGCGCACGGAATACAAAGcc > 1:3528975/1‑71 (MQ=255) gACTATTGCTATTCGGTTTATCCCCGATGGCGCGGGGAaca > 1:4951673/1‑41 (MQ=255) cTATTGCTATTCGGTTTATCCCCGATGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGc > 1:1628719/1‑71 (MQ=255) tATTGCTATTCGGTTTATCCCCGATGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCt > 1:859214/1‑71 (MQ=255) aTTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGgcg < 1:4885843/43‑1 (MQ=255) ttGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGc < 1:4117152/57‑1 (MQ=255) tGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCgg > 1:1059053/1‑71 (MQ=255) ttCGGTTTATCCCCGATGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCgt < 1:4946004/54‑1 (MQ=255) ttCGGTTTATCCCCGATGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCgt < 1:1026899/54‑1 (MQ=255) cGGTTTATCCCCGATGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTAt > 1:3542086/1‑56 (MQ=255) cGGTTTATCCCCGATGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATcc > 1:3127517/1‑71 (MQ=255) cGGTTTATCCCCGATGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATcc > 1:2314450/1‑71 (MQ=255) ccccGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTgga > 1:4282570/1‑70 (MQ=255) | GCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGC > W3110S.gb/2902742‑2902869 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |