| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,951,877 | G→A | 28.3% | A928V (GCT→GTT) | recB ← | exonuclease V (RecBCD complex), beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,951,877 | 0 | G | A | 28.3% | 86.9 / 37.3 | 53 | A928V (GCT→GTT) | recB | exonuclease V (RecBCD complex), beta subunit |
| Reads supporting (aligned to +/- strand): ref base G (20/18); new base A (8/7); total (28/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
CGGTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTAGC > W3110S.gb/2951807‑2951946 | cGGTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTgcag > 1:4699545/1‑71 (MQ=255)cGGTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTgcag > 1:1459244/1‑71 (MQ=255)cGGTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTgcag > 1:398116/1‑71 (MQ=255)cGGTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTgcag > 1:2376513/1‑71 (MQ=255) ggTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTgcag > 1:5061671/1‑70 (MQ=255) ggTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTgcag > 1:3176567/1‑70 (MQ=255) tGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCAt > 1:2655481/1‑71 (MQ=255) aCGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATc < 1:4602331/70‑1 (MQ=255) aCGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATc < 1:2609274/70‑1 (MQ=255) aCGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCg < 1:4818902/71‑1 (MQ=255) aCGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATc < 1:976131/70‑1 (MQ=255) aCCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGa < 1:3252264/68‑1 (MQ=255) aCCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGa < 1:2365209/68‑1 (MQ=255) aCCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACAt > 1:1311832/1‑71 (MQ=255) ccGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATc < 1:1222869/71‑1 (MQ=255) gcgcgGAAACTGATGTTGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCa < 1:2538375/71‑1 (MQ=255) gcgcgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATc > 1:2826103/1‑63 (MQ=255) gcgcgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATc > 1:1690262/1‑63 (MQ=255) gcgcgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCa < 1:1490761/71‑1 (MQ=255) cgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGcc < 1:238952/71‑1 (MQ=255) cgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGcc > 1:285843/1‑71 (MQ=255) cgGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGcc > 1:4787436/1‑71 (MQ=255) aaCTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAgg < 1:155742/71‑1 (MQ=255) aTGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCa < 1:3188753/71‑1 (MQ=255) tgtgGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCaa < 1:1742645/71‑1 (MQ=255) tggtgTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGcc < 1:1585024/59‑1 (MQ=255) ttAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCt < 1:1937736/69‑1 (MQ=255) aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTggg > 1:3369229/1‑70 (MQ=255) aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTggg > 1:191040/1‑70 (MQ=255) aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTggg > 1:2203508/1‑70 (MQ=255) aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGc > 1:1223435/1‑71 (MQ=255) aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGc > 1:1990578/1‑71 (MQ=255) aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGc > 1:3033210/1‑71 (MQ=255) aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGc > 1:2712459/1‑71 (MQ=255) aaCGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTggg < 1:4848315/70‑1 (MQ=255) gTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGAt < 1:2662578/71‑1 (MQ=255) tCGGTTCTTCAACGACGCTTGCAACGCCTGCAACATCGACATCCAGCCGAGGCa > 1:4497307/1‑54 (MQ=255) tCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATa > 1:2347082/1‑71 (MQ=255) tCGGTTCTTCAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATa > 1:3005629/1‑71 (MQ=255) ttcttcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGt < 1:1356566/71‑1 (MQ=255) cttcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGa > 1:1103755/1‑71 (MQ=255) cttcAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCt < 1:3586626/57‑1 (MQ=255) ttcAACGACGCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCt < 1:942785/56‑1 (MQ=255) tcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATcc > 1:308882/1‑54 (MQ=255) tcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATcc > 1:3236722/1‑54 (MQ=255) tcAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGAc > 1:4977533/1‑70 (MQ=255) acgacgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACg > 1:2917222/1‑71 (MQ=255) acgacgCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACg > 1:3955861/1‑71 (MQ=255) gacgCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCt > 1:3150395/1‑71 (MQ=255) gCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGtt > 1:3584309/1‑71 (MQ=255) gCTGGCAACGCCTGCAACATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGtt > 1:63598/1‑71 (MQ=255) aCGCCTGCAACATCGACATCCAGCCGAGGCATCAAATc > 1:3302693/1‑38 (MQ=255) aCGCCTGCAACATCGACATCCAGCCGAGGCATCAAATc > 1:270840/1‑38 (MQ=255) gCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCag < 1:1506439/71‑1 (MQ=255) gCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCag < 1:1916894/71‑1 (MQ=255) gCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCag < 1:4771044/71‑1 (MQ=255) cTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCagag < 1:4642779/71‑1 (MQ=255) cTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCagag > 1:2536446/1‑71 (MQ=255) cTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCagag < 1:2176746/71‑1 (MQ=255) cagcaTCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTAg > 1:4227451/1‑71 (MQ=255) agcaTCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTAGc < 1:3517067/71‑1 (MQ=255) agcaTCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTAGc < 1:1322877/71‑1 (MQ=255) | CGGTGACGCACCGCGCGGAAACTGATGTGGTGTTAACGTCGGTTCTTCAACGACGCTGGCAACGCCTGCAGCATCGACATCCAGCCGAGGCATCAAATCCTGGGCGATACCGTGACCACGCTGTTGCAAACCAGAGTAGC > W3110S.gb/2951807‑2951946 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |