| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,966,215 | T→G | 42.9% | K292N (AAA→AAC) | ptsP ← | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,966,215 | 0 | T | G | 42.9% | 3.5 / 100.6 | 63 | K292N (AAA→AAC) | ptsP | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Reads supporting (aligned to +/- strand): ref base T (20/16); new base G (14/13); total (34/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCAC > W3110S.gb/2966147‑2966284 | ccTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCttt < 1:2678344/71‑1 (MQ=255)ccTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCttt < 1:2813241/71‑1 (MQ=255)ccTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCttt < 1:4198410/71‑1 (MQ=255)ccTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCttt < 1:3619194/71‑1 (MQ=255)ccTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCgtt > 1:5109901/1‑71 (MQ=255) ttGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCttt < 1:1859181/69‑1 (MQ=255) gATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCgtt > 1:1599089/1‑67 (MQ=255) gATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCgtt > 1:1590126/1‑67 (MQ=255) gATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCgtt > 1:2274688/1‑67 (MQ=255) gATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTgag < 1:3945683/70‑1 (MQ=255) aTTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTga < 1:414504/68‑1 (MQ=255) aTTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTga < 1:411332/68‑1 (MQ=255) aGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGt > 1:4477842/1‑71 (MQ=255) atcaAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCAc > 1:3996902/1‑71 (MQ=255) atcaAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCAc > 1:3138633/1‑71 (MQ=255) caAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCAc > 1:4082246/1‑69 (MQ=255) caAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACt > 1:952115/1‑70 (MQ=255) caAGATGAAACAGCAATCGCTGACACAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACt > 1:3718437/1‑70 (MQ=255) aaGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACtt < 1:2292755/70‑1 (MQ=255) aGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAg > 1:4564360/1‑71 (MQ=255) tGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAgcgc < 1:1410078/71‑1 (MQ=255) aCAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCa < 1:2725941/71‑1 (MQ=255) gCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGtt < 1:4454529/52‑1 (MQ=255) gCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCaaa < 1:4809324/70‑1 (MQ=255) gCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCaa < 1:484888/69‑1 (MQ=255) gCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCAc < 1:347808/57‑1 (MQ=255) cAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATa > 1:2614841/1‑48 (MQ=255) tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGt < 1:2357338/70‑1 (MQ=255) tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAg < 1:2122348/46‑1 (MQ=255) tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAg < 1:1809917/46‑1 (MQ=255) tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAg < 1:3216040/46‑1 (MQ=255) tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCa < 1:1177116/64‑1 (MQ=255) tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCa < 1:1222282/64‑1 (MQ=255) cGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTAt > 1:2907087/1‑49 (MQ=255) gCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCg > 1:1873541/1‑71 (MQ=255) gCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCg > 1:4461100/1‑71 (MQ=255) aCCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGt < 1:48340/41‑1 (MQ=255) aCCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGt < 1:1790646/41‑1 (MQ=255) cAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATa > 1:1856028/1‑36 (MQ=255) aGCGCACGTAAATCGCCCGCCCGCTCTTTGAGATAGTTa > 1:1388126/1‑39 (MQ=255) aGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAAttt > 1:226852/1‑71 (MQ=255) gcgcACGTAAATCGCCAGCCCTCTCGTTGAGATAGTTATCACTTAGCGCGGCAAAc < 1:4867296/56‑1 (MQ=255) gcgcACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTg > 1:3421806/1‑58 (MQ=255) gcgcACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTg > 1:1829421/1‑58 (MQ=255) gcgcACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTg > 1:1570842/1‑58 (MQ=255) gcgcACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAAtttt < 1:2086402/71‑1 (MQ=255) cGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAAt > 1:1471991/1‑71 (MQ=255) gTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTa > 1:2785107/1‑39 (MQ=255) tAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCaaa > 1:2172167/1‑48 (MQ=255) aaaTCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAgcg > 1:263824/1‑40 (MQ=255) aaaTCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCaaa > 1:2210858/1‑47 (MQ=255) aaaTCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTc > 1:4320741/1‑65 (MQ=255) aaaTCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTc > 1:4884222/1‑65 (MQ=255) aaaTCGCCAGCCCGCTCTTAGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCaa > 1:267340/1‑67 (MQ=255) aTCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCg > 1:4207276/1‑71 (MQ=255) tCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGa > 1:2885023/1‑67 (MQ=255) cGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGtt > 1:4200784/1‑71 (MQ=255) gCCAGCCCGCTCTTTGAGATAGTTATCAGTTAGCGCGGCAAACTGTTGGGCAAATTTTTCaa > 1:3579174/1‑62 (MQ=255) gCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCGAATTTTTCAATGACCGtt < 1:3647056/70‑1 (MQ=255) ccAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGt < 1:3380279/45‑1 (MQ=255) aGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTc > 1:4741888/1‑45 (MQ=255) aGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTc > 1:3144826/1‑45 (MQ=255) aGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTa < 1:1020146/71‑1 (MQ=255) gCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTc < 1:2985364/56‑1 (MQ=255) gCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGttt > 1:5034760/1‑67 (MQ=255) cccGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTAc < 1:2397225/70‑1 (MQ=255) cccGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCa > 1:3183940/1‑56 (MQ=255) cccGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCa > 1:2036931/1‑56 (MQ=255) gCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAAt < 1:1851529/47‑1 (MQ=255) gCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAAt < 1:4849408/47‑1 (MQ=255) gCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAAt < 1:2901193/47‑1 (MQ=255) gCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGt < 1:4515051/61‑1 (MQ=255) ctTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCAc > 1:2379535/1‑71 (MQ=255) ctTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCAc > 1:497973/1‑71 (MQ=255) tttGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTc < 1:4202717/48‑1 (MQ=255) | CCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCAC > W3110S.gb/2966147‑2966284 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |