Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,966,215 T→G 42.9% K292N (AAA→AAC ptsP ← fused PEP‑protein phosphotransferase (enzyme I) of PTS system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,966,2150TG42.9% 3.5 / 100.6 63K292N (AAA→AACptsPfused PEP‑protein phosphotransferase (enzyme I) of PTS system
Reads supporting (aligned to +/- strand):  ref base T (20/16);  new base G (14/13);  total (34/29)
Fisher's exact test for biased strand distribution p-value = 8.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCAC  >  W3110S.gb/2966147‑2966284
                                                                    |                                                                     
ccTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCttt                                                                     <  1:2678344/71‑1 (MQ=255)
ccTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCttt                                                                     <  1:2813241/71‑1 (MQ=255)
ccTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCttt                                                                     <  1:4198410/71‑1 (MQ=255)
ccTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCttt                                                                     <  1:3619194/71‑1 (MQ=255)
ccTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCgtt                                                                     >  1:5109901/1‑71 (MQ=255)
  ttGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCttt                                                                     <  1:1859181/69‑1 (MQ=255)
    gATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCgtt                                                                     >  1:1599089/1‑67 (MQ=255)
    gATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCgtt                                                                     >  1:1590126/1‑67 (MQ=255)
    gATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCgtt                                                                     >  1:2274688/1‑67 (MQ=255)
    gATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTgag                                                                  <  1:3945683/70‑1 (MQ=255)
     aTTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTga                                                                   <  1:414504/68‑1 (MQ=255)
     aTTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTga                                                                   <  1:411332/68‑1 (MQ=255)
        aGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGt                                                             >  1:4477842/1‑71 (MQ=255)
              atcaAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCAc                                                       >  1:3996902/1‑71 (MQ=255)
              atcaAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCAc                                                       >  1:3138633/1‑71 (MQ=255)
                caAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCAc                                                       >  1:4082246/1‑69 (MQ=255)
                caAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACt                                                      >  1:952115/1‑70 (MQ=255)
                caAGATGAAACAGCAATCGCTGACACAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACt                                                      >  1:3718437/1‑70 (MQ=255)
                 aaGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACtt                                                     <  1:2292755/70‑1 (MQ=255)
                  aGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAg                                                   >  1:4564360/1‑71 (MQ=255)
                     tGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAgcgc                                                <  1:1410078/71‑1 (MQ=255)
                         aCAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCa                                            <  1:2725941/71‑1 (MQ=255)
                            gCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGtt                                                            <  1:4454529/52‑1 (MQ=255)
                            gCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCaaa                                          <  1:4809324/70‑1 (MQ=255)
                            gCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCaa                                           <  1:484888/69‑1 (MQ=255)
                            gCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCAc                                                       <  1:347808/57‑1 (MQ=255)
                             cAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATa                                                               >  1:2614841/1‑48 (MQ=255)
                                tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGt                                      <  1:2357338/70‑1 (MQ=255)
                                tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAg                                                              <  1:2122348/46‑1 (MQ=255)
                                tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAg                                                              <  1:1809917/46‑1 (MQ=255)
                                tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAg                                                              <  1:3216040/46‑1 (MQ=255)
                                tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCa                                            <  1:1177116/64‑1 (MQ=255)
                                tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCa                                            <  1:1222282/64‑1 (MQ=255)
                                 cGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTAt                                                          >  1:2907087/1‑49 (MQ=255)
                                  gCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCg                                   >  1:1873541/1‑71 (MQ=255)
                                  gCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCg                                   >  1:4461100/1‑71 (MQ=255)
                                      aCCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGt                                                             <  1:48340/41‑1 (MQ=255)
                                      aCCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGt                                                             <  1:1790646/41‑1 (MQ=255)
                                         cAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATa                                                               >  1:1856028/1‑36 (MQ=255)
                                          aGCGCACGTAAATCGCCCGCCCGCTCTTTGAGATAGTTa                                                           >  1:1388126/1‑39 (MQ=255)
                                          aGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAAttt                           >  1:226852/1‑71 (MQ=255)
                                           gcgcACGTAAATCGCCAGCCCTCTCGTTGAGATAGTTATCACTTAGCGCGGCAAAc                                         <  1:4867296/56‑1 (MQ=255)
                                           gcgcACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTg                                       >  1:3421806/1‑58 (MQ=255)
                                           gcgcACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTg                                       >  1:1829421/1‑58 (MQ=255)
                                           gcgcACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTg                                       >  1:1570842/1‑58 (MQ=255)
                                           gcgcACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAAtttt                          <  1:2086402/71‑1 (MQ=255)
                                                cGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAAt                     >  1:1471991/1‑71 (MQ=255)
                                                 gTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTa                                                    >  1:2785107/1‑39 (MQ=255)
                                                  tAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCaaa                                          >  1:2172167/1‑48 (MQ=255)
                                                   aaaTCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAgcg                                                 >  1:263824/1‑40 (MQ=255)
                                                   aaaTCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCaaa                                          >  1:2210858/1‑47 (MQ=255)
                                                   aaaTCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTc                        >  1:4320741/1‑65 (MQ=255)
                                                   aaaTCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTc                        >  1:4884222/1‑65 (MQ=255)
                                                   aaaTCGCCAGCCCGCTCTTAGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCaa                      >  1:267340/1‑67 (MQ=255)
                                                     aTCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCg                >  1:4207276/1‑71 (MQ=255)
                                                      tCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGa                   >  1:2885023/1‑67 (MQ=255)
                                                       cGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGtt              >  1:4200784/1‑71 (MQ=255)
                                                        gCCAGCCCGCTCTTTGAGATAGTTATCAGTTAGCGCGGCAAACTGTTGGGCAAATTTTTCaa                      >  1:3579174/1‑62 (MQ=255)
                                                        gCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCGAATTTTTCAATGACCGtt              <  1:3647056/70‑1 (MQ=255)
                                                         ccAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGt                                      <  1:3380279/45‑1 (MQ=255)
                                                           aGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTc                                    >  1:4741888/1‑45 (MQ=255)
                                                           aGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTc                                    >  1:3144826/1‑45 (MQ=255)
                                                           aGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTa          <  1:1020146/71‑1 (MQ=255)
                                                            gCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTc                        <  1:2985364/56‑1 (MQ=255)
                                                            gCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGttt             >  1:5034760/1‑67 (MQ=255)
                                                             cccGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTAc         <  1:2397225/70‑1 (MQ=255)
                                                             cccGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCa                       >  1:3183940/1‑56 (MQ=255)
                                                             cccGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCa                       >  1:2036931/1‑56 (MQ=255)
                                                                gCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAAt                             <  1:1851529/47‑1 (MQ=255)
                                                                gCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAAt                             <  1:4849408/47‑1 (MQ=255)
                                                                gCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAAt                             <  1:2901193/47‑1 (MQ=255)
                                                                gCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGt               <  1:4515051/61‑1 (MQ=255)
                                                                   ctTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCAc  >  1:2379535/1‑71 (MQ=255)
                                                                   ctTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCAc  >  1:497973/1‑71 (MQ=255)
                                                                    tttGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTc                        <  1:4202717/48‑1 (MQ=255)
                                                                    |                                                                     
CCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCAC  >  W3110S.gb/2966147‑2966284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: