| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,972,729 | T→C | 46.1% | S648G (AGC→GGC) | aas ← | fused 2‑acylglycerophospho‑ethanolamine acyl transferase and acyl‑acyl carrier protein synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,972,729 | 0 | T | C | 46.1% | 7.0 / 109.5 | 76 | S648G (AGC→GGC) | aas | fused 2‑acylglycerophospho‑ethanolamine acyl transferase and acyl‑acyl carrier protein synthetase |
| Reads supporting (aligned to +/- strand): ref base T (22/19); new base C (19/16); total (41/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.92e-01 | |||||||||||
TGCAACTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGC > W3110S.gb/2972659‑2972796 | tGCAACTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGct > 1:3148887/1‑71 (MQ=255) aaCTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCtt < 1:4786714/71‑1 (MQ=255) cTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTaa > 1:2960524/1‑71 (MQ=255) cTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTaa > 1:2737215/1‑71 (MQ=255) ttATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAAt > 1:336917/1‑71 (MQ=255) tATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTa > 1:2770477/1‑68 (MQ=255) tATCGAGCGTCAGTTCGTTATCTGTGGTGAAAAGCAACAGTGCCTCGCCTTTGCTGGCATCGCCCTTa > 1:3928891/1‑68 (MQ=255) tCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCg < 1:1912984/70‑1 (MQ=255) tCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGc < 1:817277/71‑1 (MQ=255) tCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGc < 1:612403/71‑1 (MQ=255) gTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTgg > 1:1867200/1‑70 (MQ=255) tCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCa < 1:4324412/71‑1 (MQ=255) cAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCAt > 1:4326355/1‑71 (MQ=255) aGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCAt < 1:4851887/70‑1 (MQ=255) aGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATg < 1:4427554/71‑1 (MQ=255) ttCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAg > 1:5008700/1‑62 (MQ=255) ttCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGt > 1:3669228/1‑63 (MQ=255) gTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACtt > 1:1704818/1‑71 (MQ=255) aTCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTa < 1:4856998/70‑1 (MQ=255) aTCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTAt > 1:4611148/1‑71 (MQ=255) aTCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTg > 1:4171018/1‑58 (MQ=255) aTCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTg > 1:1986192/1‑58 (MQ=255) tCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATGGACTTTATc > 1:4545521/1‑71 (MQ=255) tCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATGGACTTTATc > 1:798152/1‑71 (MQ=255) tCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATGGACTTTATc > 1:5144837/1‑71 (MQ=255) tggtgAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTaa > 1:2686062/1‑45 (MQ=255) tggtgAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCt < 1:4230949/68‑1 (MQ=255) tggtgAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTg < 1:4047131/69‑1 (MQ=255) gtgAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTTGc < 1:4066304/53‑1 (MQ=255) gtgAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATg < 1:4762458/56‑1 (MQ=255) gtgAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATg < 1:2631005/56‑1 (MQ=255) gtgAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATg < 1:2515060/56‑1 (MQ=255) gtgAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATg < 1:1131309/56‑1 (MQ=255) gtgAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATGGACTTTATCTGGCGa < 1:2113574/71‑1 (MQ=255) gtgAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATGGACTTTATCTCGCGa < 1:2130138/71‑1 (MQ=255) tgAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACt < 1:2506202/59‑1 (MQ=255) tgAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATg < 1:2915599/55‑1 (MQ=255) aaaaGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGaaa > 1:4339652/1‑70 (MQ=255) aaaaGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCtt < 1:3551999/38‑1 (MQ=255) aaaaGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCtt < 1:1867447/38‑1 (MQ=255) aaaaGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGc < 1:2792917/44‑1 (MQ=255) aaaaGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATGGACTTTATCTGGc < 1:622368/66‑1 (MQ=255) aaaGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAg > 1:4041752/1‑45 (MQ=255) aaaGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAg > 1:4838563/1‑45 (MQ=255) aGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCa > 1:1298024/1‑42 (MQ=255) aGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCa > 1:4848742/1‑42 (MQ=255) aGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACAcc < 1:4582178/71‑1 (MQ=255) aGCACCAGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAacac > 1:5120379/1‑70 (MQ=255) aCCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTgg > 1:701934/1‑43 (MQ=255) aCCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAg > 1:2780286/1‑71 (MQ=255) aCCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAg > 1:2712469/1‑71 (MQ=255) ccAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGa < 1:5121947/61‑1 (MQ=255) aGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATGGACTTTATCTGGCGaaa > 1:3472321/1‑61 (MQ=255) aGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATGGACTTTATCTGGCGaaa > 1:884370/1‑61 (MQ=255) aGTGCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATGGACTTTATCTGGCGaaa > 1:454483/1‑61 (MQ=255) gTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGaaa > 1:1849740/1‑60 (MQ=255) gCCTCGCCTTTGCTGGCATCGCCCTTAATCGCGGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAg > 1:1127310/1‑71 (MQ=255) gCCTCGCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAg > 1:5097339/1‑71 (MQ=255) ccTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAgt < 1:1687135/71‑1 (MQ=255) cTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAgt < 1:1478209/70‑1 (MQ=255) gCCTTTGCTGGCATCGCCCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAgttgtt > 1:4965639/1‑71 (MQ=255) tGCTGGCATCGCCCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTccac > 1:2266396/1‑70 (MQ=255) tGCTGGCATCGCCCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTcca > 1:2012209/1‑69 (MQ=255) tGCTGGCATCGCCCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTcca < 1:5072635/69‑1 (MQ=255) gCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTc < 1:2802579/71‑1 (MQ=255) gCATCGCCCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTccac > 1:2596231/1‑65 (MQ=255) gCATCGCCCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTc < 1:5010810/71‑1 (MQ=255) cATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTcc < 1:3791755/71‑1 (MQ=255) tCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCa < 1:5007271/45‑1 (MQ=255) tCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCa < 1:1949736/45‑1 (MQ=255) gCTCTTAATCGCAGTGGCATGGACTTTATCTGGCg > 1:1491058/1‑35 (MQ=255) gCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGa < 1:906999/36‑1 (MQ=255) gCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGa < 1:3302524/36‑1 (MQ=255) gCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAgt > 1:202097/1‑52 (MQ=255) gCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGc > 1:3175910/1‑70 (MQ=255) gCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGc > 1:259131/1‑70 (MQ=255) gCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGc > 1:2457227/1‑70 (MQ=255) ctctTAATCGCAGTGGCATGGACTTTATCTGGCGa < 1:2083019/35‑1 (MQ=255) | TGCAACTTATCGCGCGTCAGTTCGTTATCTGTGGTGAAAAGCACCAGTGCCTCGCCTTTGCTGGCATCGCTCTTAATCGCAGTGGCATGGACTTTATCTGGCGAAACACCAAGTGCCAGTTGTTCCACCATTTCCAGC > W3110S.gb/2972659‑2972796 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |