| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,001,511 | T→C | 40.0% | I81T (ATA→ACA) | xdhB → | xanthine dehydrogenase, FAD‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,001,511 | 0 | T | C | 40.0% | 54.4 / 111.0 | 90 | I81T (ATA→ACA) | xdhB | xanthine dehydrogenase, FAD‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base T (36/18); new base C (24/12); total (60/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
CGGGGAATTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGACCG > W3110S.gb/3001444‑3001578 | cGGGGAATTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCAAGCTAATaga < 1:1824291/71‑1 (MQ=255) aCGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCta < 1:1129934/70‑1 (MQ=255) aCGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCta > 1:1240782/1‑70 (MQ=255) aCGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCta > 1:3117609/1‑70 (MQ=255) aCGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCta > 1:4473515/1‑70 (MQ=255) aCGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCta > 1:970347/1‑70 (MQ=255) cGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCtat > 1:3013124/1‑70 (MQ=255) gCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATa > 1:2530954/1‑54 (MQ=255) gCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGAt > 1:4344790/1‑60 (MQ=255) gCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGAt > 1:1521333/1‑60 (MQ=255) gCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGAt > 1:4641082/1‑60 (MQ=255) ggAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATaga < 1:1704616/54‑1 (MQ=255) ggAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATaga < 1:4395595/54‑1 (MQ=255) ggAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAAc < 1:3667171/71‑1 (MQ=255) gAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATaga < 1:25601/53‑1 (MQ=255) aGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGt > 1:522545/1‑70 (MQ=255) aGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGt > 1:1407824/1‑70 (MQ=255) gATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATaga < 1:2278502/50‑1 (MQ=255) tGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATagaa > 1:3405488/1‑49 (MQ=255) tGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGAt < 1:4188457/52‑1 (MQ=255) tGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGAt < 1:2371831/52‑1 (MQ=255) tGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCtat > 1:1165722/1‑57 (MQ=255) ggCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACagaa > 1:820607/1‑48 (MQ=255) ggCTCGCTACGTATCGGCTCTGCAACGACATTTACCAAGCTAATAGAAGATCCTATaa > 1:5090174/1‑58 (MQ=255) gCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCAtctc > 1:2945412/1‑71 (MQ=255) cTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCAtct > 1:839200/1‑69 (MQ=255) tCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATagaa > 1:2023949/1‑45 (MQ=255) tCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATaa > 1:2526204/1‑55 (MQ=255) gCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCAt > 1:2015325/1‑64 (MQ=255) gCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCAt > 1:2009445/1‑64 (MQ=255) cTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc < 1:3286547/71‑1 (MQ=255) cTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc < 1:467790/71‑1 (MQ=255) cTACGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc < 1:3414261/71‑1 (MQ=255) cGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGc < 1:2372206/68‑1 (MQ=255) cGTATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGtt < 1:204208/71‑1 (MQ=255) tATCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCa < 1:4043078/51‑1 (MQ=255) tCGGCTCTGCAACGACATTTACCCAGCTAATagaag > 1:2444203/1‑36 (MQ=255) tCGGCTCTGCAACGACATTTACCCAGCTAATagaag > 1:4399874/1‑36 (MQ=255) tCGGCTCTGCAACGACATTTACCCAGCTAATagaag > 1:1532067/1‑36 (MQ=255) tCGGCTCTGCAACGACATTTACCCAGCTAATagaa > 1:5124002/1‑35 (MQ=255) tCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTAtgt > 1:3710183/1‑71 (MQ=255) tCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTAtgt > 1:3716015/1‑71 (MQ=255) tCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTAtgt > 1:3226150/1‑71 (MQ=255) tCGGCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCta > 1:2108180/1‑42 (MQ=255) ggCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCa > 1:4014914/1‑53 (MQ=255) ggCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCAt > 1:5007058/1‑54 (MQ=255) ggCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCg > 1:2871118/1‑63 (MQ=255) ggCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGtgc > 1:3501352/1‑71 (MQ=255) ggCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTAtgtg < 1:1630905/70‑1 (MQ=255) gCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGtgct < 1:3669830/71‑1 (MQ=255) gCTCTGCAACGACATTTACCCAGCTAACAGAAGATCCtat < 1:1982293/40‑1 (MQ=255) tctGCATCGACATTTACCCAGCTAACAGAAGATCCTATAACTc < 1:3329187/43‑1 (MQ=255) tGCAACGACATTTACCCAGCTAATAGAAGATCCtat < 1:3590412/36‑1 (MQ=255) tGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGtgc < 1:2363327/66‑1 (MQ=255) tGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCgg > 1:3334805/1‑71 (MQ=255) tGCAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCgg > 1:619358/1‑71 (MQ=255) gCAACGACATTTCCCCAGCTAACAGAAGATCCTATAACTCAACGTCAt > 1:3698478/1‑48 (MQ=255) gCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGc > 1:1909667/1‑71 (MQ=255) cAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTGAt > 1:4556794/1‑47 (MQ=255) cAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCAt > 1:3495332/1‑47 (MQ=255) cAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCAt > 1:5131149/1‑47 (MQ=255) cAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCAt > 1:495924/1‑47 (MQ=255) cAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCAt > 1:1515820/1‑47 (MQ=255) cAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGc < 1:349867/70‑1 (MQ=255) cAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTAAt > 1:4551004/1‑47 (MQ=255) cAACGACATTTACCCAGCTAACAGAAGATCCTATaa > 1:3566324/1‑36 (MQ=255) cAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTAtg < 1:3286731/61‑1 (MQ=255) cAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGc > 1:546688/1‑70 (MQ=255) cAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGc > 1:71356/1‑70 (MQ=255) cAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGc > 1:3574396/1‑70 (MQ=255) cAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGc > 1:2264860/1‑70 (MQ=255) cAACGACATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGc > 1:279849/1‑70 (MQ=255) aCGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCAc > 1:1789791/1‑71 (MQ=255) aCATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTc < 1:4083792/71‑1 (MQ=255) aCATTTACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTc < 1:4155071/71‑1 (MQ=255) ttACCCAGCTAACAGAAGATCCTATAACTCAACGTCAt > 1:279106/1‑38 (MQ=255) ttACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCAtt > 1:2805557/1‑71 (MQ=255) tACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCAtt > 1:349715/1‑70 (MQ=255) tACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTg > 1:427798/1‑71 (MQ=255) tACCCAGCTAACTGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTAt > 1:3421619/1‑50 (MQ=255) tACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTAt > 1:689452/1‑50 (MQ=255) tACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTAt > 1:3518245/1‑50 (MQ=255) tACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTg > 1:728224/1‑71 (MQ=255) tACCCAGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTg > 1:1765850/1‑71 (MQ=255) ccAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTg < 1:983236/71‑1 (MQ=255) ccAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTg < 1:5016413/71‑1 (MQ=255) ccAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTg < 1:1142212/71‑1 (MQ=255) aGCTAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGa < 1:2651459/71‑1 (MQ=255) tAACAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGACCg > 1:1489764/1‑71 (MQ=255) aTAGAAGATCCTATAACTCAACGTCATCTTCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTg < 1:4748208/64‑1 (MQ=255) tAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTa < 1:4001654/38‑1 (MQ=255) tAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGACCg > 1:289322/1‑68 (MQ=255) tAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGACCg > 1:18321/1‑68 (MQ=255) | CGGGGAATTACGCTGGCGGAAGATGGCTCGCTACGTATCGGCTCTGCAACGACATTTACCCAGCTAATAGAAGATCCTATAACTCAACGTCATCTCCCGGCGTTATGTGCTGCGGCCACGTCCATTGCTGGACCG > W3110S.gb/3001444‑3001578 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |