| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,007,077 | C→T | 34.7% | N304N (AAC→AAT) | ygeX → | 2,3‑diaminopropionate ammonia‑lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,007,077 | 0 | C | T | 34.7% | 88.1 / 72.9 | 75 | N304N (AAC→AAT) | ygeX | 2,3‑diaminopropionate ammonia‑lyase |
| Reads supporting (aligned to +/- strand): ref base C (28/21); new base T (17/9); total (45/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAAT > W3110S.gb/3007010‑3007143 | tGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAATTgc > 1:756289/1‑71 (MQ=255)tGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAATTg < 1:3705075/70‑1 (MQ=255) gCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAACTgcgc < 1:951175/71‑1 (MQ=255) atcatGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAATTgcg > 1:752984/1‑64 (MQ=255) atcatGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAATTGCGCCAccc > 1:1150757/1‑70 (MQ=255) atcatGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAATTGCGCCAccc > 1:4372790/1‑70 (MQ=255) atcatGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAATTGCGCCAccc > 1:4559775/1‑70 (MQ=255) atcatGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCAccc > 1:2621546/1‑70 (MQ=255) tcatGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAATTGCGCCACCCaa > 1:4142527/1‑71 (MQ=255) tcatGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAATTGCGCCACCCaa > 1:427842/1‑71 (MQ=255) tcatGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCaa < 1:3333118/71‑1 (MQ=255) atGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAAtt > 1:3168549/1‑71 (MQ=255) atGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAAtt > 1:4279709/1‑71 (MQ=255) atGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAAtt > 1:2672611/1‑71 (MQ=255) atGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAAtt > 1:2984530/1‑71 (MQ=255) atGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAAtt > 1:4494349/1‑71 (MQ=255) atGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAAt > 1:3375460/1‑70 (MQ=255) gCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAATTGCGCCACCCAATTCAt > 1:580984/1‑71 (MQ=255) gCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAATTCa < 1:692220/70‑1 (MQ=255) cAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAATTgcgc > 1:3825202/1‑58 (MQ=255) cAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAATTCAtc < 1:4324490/71‑1 (MQ=255) ctggcctgTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTg < 1:627204/71‑1 (MQ=255) gcctgTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAATTGCGCCACCCAAtt > 1:3004159/1‑59 (MQ=255) gcctgTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAATTGCGCCACCCAAtt > 1:977602/1‑59 (MQ=255) gcctgTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGcc < 1:937287/70‑1 (MQ=255) cctgTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAg < 1:1786883/71‑1 (MQ=255) tgtgGCGAACCTAACCCGCTGGGCTGTGAAATCCTACGTAATTGCGCCACCCAATTCATCTCCTGCCAg < 1:3246919/69‑1 (MQ=255) tgtgGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAATTGCGCCACCCAAtt < 1:1932300/56‑1 (MQ=255) tgGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAATTGCGCCACCCAATTCATCTCCTGCCAGGa < 1:3531606/69‑1 (MQ=255) tgGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGAc < 1:1979902/70‑1 (MQ=255) gCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACacc > 1:5118713/1‑69 (MQ=255) gCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGc > 1:3619311/1‑71 (MQ=255) gCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGc > 1:2415207/1‑71 (MQ=255) gCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGc > 1:3400642/1‑71 (MQ=255) cGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCg < 1:1254018/71‑1 (MQ=255) aaCCTAACCCGCTGGGCTGGGAAATCCTACGTAATTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGtt > 1:1972103/1‑71 (MQ=255) aaCCTAACCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAg > 1:5079525/1‑62 (MQ=255) ccTAACCCGCTGGGCTGGGAAATCCTACGTAATTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGt > 1:648151/1‑68 (MQ=255) ccTAACCCGCTGGGCTGGGAAATCCTACGTAATTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGt > 1:395557/1‑68 (MQ=255) aaCCCGCTGGGCTGGGAAATCCTACGTAATTGCGCCACCCAATTCa > 1:174927/1‑46 (MQ=255) aaCCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGt > 1:2097405/1‑65 (MQ=255) aaCCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCg > 1:1635206/1‑70 (MQ=255) aaCACGCTGGGCTGGGAAATCCTACGTAACTgcgc > 1:2376084/1‑35 (MQ=255) aCCCGCTGGGCTGGGAAATCCTACGTAATTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCg < 1:4131919/69‑1 (MQ=255) aCCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGc < 1:3578044/70‑1 (MQ=255) cccGCTGGGCTGGGAAATCCTACGTAATTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCAt < 1:3900419/71‑1 (MQ=255) cccGCTGGGCTGGGAAATCCTACGTAATTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCAt < 1:447309/71‑1 (MQ=255) cccGCTGGGCTGGGAAATCCTACGTAATTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCAt < 1:3412895/71‑1 (MQ=255) cccGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAATTc < 1:972329/43‑1 (MQ=255) cccGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAATTc < 1:4672807/43‑1 (MQ=255) ccGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCAtt < 1:167009/71‑1 (MQ=255) ccGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCAt < 1:2816502/70‑1 (MQ=255) cGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCAt > 1:2992933/1‑69 (MQ=255) ctgggctgggAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAgg < 1:764941/71‑1 (MQ=255) ctgggctgggAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAgg < 1:3043408/71‑1 (MQ=255) tgggctgggAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAgg < 1:4735290/70‑1 (MQ=255) gggctgggAAATCCTACGTAATTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAg > 1:939375/1‑68 (MQ=255) gggctgggAAATCCTACGTAACTGCGCCACCCAATTCa > 1:2541195/1‑38 (MQ=255) gggctgggAAATCCTACGTAACTGCGCCACCCAATTCAt > 1:4861570/1‑39 (MQ=255) tgggAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGcag > 1:4248010/1‑42 (MQ=255) tgggAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAg > 1:510704/1‑45 (MQ=255) gggAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATgcgc > 1:2228707/1‑71 (MQ=255) gggAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATgcgc > 1:3947261/1‑71 (MQ=255) ggAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGcgcg > 1:1611359/1‑71 (MQ=255) aaaTCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAgg > 1:2893701/1‑61 (MQ=255) aaaTCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTg > 1:943022/1‑71 (MQ=255) aTCCTACGTAATTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCt < 1:2455454/71‑1 (MQ=255) aTCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCt > 1:994824/1‑71 (MQ=255) tCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATg < 1:2661246/62‑1 (MQ=255) tCCTACGAAATTGCGCCACCCAATTCATCTCCTGcc > 1:4757669/1‑36 (MQ=37) ccTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTgg > 1:2816852/1‑71 (MQ=255) cTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGt < 1:2328541/65‑1 (MQ=255) cTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGt < 1:3224952/65‑1 (MQ=255) cGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTga > 1:1262803/1‑43 (MQ=255) tAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTatt < 1:4785004/70‑3 (MQ=255) | TGGCCACCATCATGGCAGGCCTGGCCTGTGGCGAACCTAACCCGCTGGGCTGGGAAATCCTACGTAACTGCGCCACCCAATTCATCTCCTGCCAGGACAGCGTTGCCGCATTAGGTATGCGCGTGCTGGGTAAT > W3110S.gb/3007010‑3007143 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |