| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,012,950 | A→G | 37.8% | V255A (GTG→GCG) | yqeC ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,012,950 | 0 | A | G | 37.8% | 25.7 / 38.1 | 37 | V255A (GTG→GCG) | yqeC | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (13/10); new base G (6/8); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTT > W3110S.gb/3012883‑3013019 | tgAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTcgca > 1:549927/1‑70 (MQ=255) aaaaTATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAAt > 1:1476598/1‑71 (MQ=255) aaaaTATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAAt > 1:5134036/1‑71 (MQ=255) aaaaTATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAAt > 1:3684434/1‑71 (MQ=255) aTACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTgcgcgc < 1:1694704/70‑1 (MQ=255) tACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCGCaa < 1:3639635/59‑1 (MQ=255) tCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCGCAAATCTGCGCGCGAtt < 1:4324999/65‑1 (MQ=255) aaTGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCGCAAATCTGCGCGCGATTGCAGGa < 1:3493989/69‑1 (MQ=255) aaTGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCGCAAATCTGCGCGCGATTGCAGGATg < 1:3765859/71‑1 (MQ=255) gATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCGCaa > 1:1071950/1‑45 (MQ=255) tatCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGTATGTTCTTg < 1:3005187/71‑1 (MQ=255) cTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCGCAAATCTGCGCGCGATTGCAGGATGTTCTTGtata > 1:2512609/1‑71 (MQ=255) tttCAGTAACCCGGAATACCCGGGCCGCTAATTCGCAAATCTGCGCGCGATTGCAGGATGTTCTTGTatat > 1:2469412/1‑71 (MQ=255) tttCAGTAACCCGGAATACCCGGGCCGCTAATTCACaa < 1:4648050/38‑1 (MQ=255) tttCAGTAACCCGGAATACCCGGG‑CGCTAATTCACaa < 1:4207870/37‑1 (MQ=38) aGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGa > 1:2382749/1‑46 (MQ=255) aaCCCGGAATACCCGGGCCGCTAATTCACAAATCTgcgcgc < 1:1260501/41‑1 (MQ=255) aaCCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCccag > 1:2307608/1‑71 (MQ=255) aCCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCccagc < 1:3922342/71‑1 (MQ=255) cccGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGa > 1:3341587/1‑41 (MQ=255) cccGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGa > 1:412102/1‑41 (MQ=255) ccGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCccagcca < 1:2908215/71‑1 (MQ=255) cGGAATACCCGGGCCGCTAATTCGCAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCccagccag < 1:2736118/71‑1 (MQ=255) ggAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCccagc > 1:4495733/1‑67 (MQ=255) tACCCGGGCCGCTAATTCGCAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGc > 1:4120450/1‑71 (MQ=255) tACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGAtt < 1:4838671/69‑1 (MQ=255) cTAATTCACAAATCTGCGCGCGATTGCAGGATGtt < 1:577830/35‑1 (MQ=255) cTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGtat < 1:2157056/42‑1 (MQ=255) cTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGcc > 1:2254084/1‑61 (MQ=255) tAATTCGCAAATCTGCGCGCGATTGCAGGATGTTCTTg < 1:4211348/38‑1 (MQ=255) aaTTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGc > 1:240629/1‑58 (MQ=255) aTTCGCAAATCTGCGCGCGATTGCAGGATGTTCTTGta > 1:3863061/1‑38 (MQ=255) aTTCGCAAATCTGCGCGCGATTGCAGGATGTTCTTGta > 1:2803741/1‑38 (MQ=255) ttCGCAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGAtt < 1:2224639/54‑1 (MQ=255) ttCGCAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGAtt < 1:1174630/54‑1 (MQ=255) tCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCccagccag > 1:4816494/1‑50 (MQ=255) tCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGa > 1:2072326/1‑51 (MQ=255) tCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGtgttgt > 1:2686722/1‑71 (MQ=255) cacaAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCATCCAGATTGCctc < 1:3713822/57‑1 (MQ=255) cacaAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTgttgtt > 1:3844102/1‑71 (MQ=255) | TGAAAATATTCATACCAACCTCAATGGATATCCTTTCAGTAACCCGGAATACCCGGGCCGCTAATTCACAAATCTGCGCGCGATTGCAGGATGTTCTTGTATATCGCCCAGCCAGATTGCCTCTACGTCGTGTTGTT > W3110S.gb/3012883‑3013019 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |