| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,018,853 | C→T | 28.1% | T346I (ACT→ATT) | ssnA → | predicted chlorohydrolase/aminohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,018,853 | 0 | C | T | 28.1% | 95.9 / 39.0 | 57 | T346I (ACT→ATT) | ssnA | predicted chlorohydrolase/aminohydrolase |
| Reads supporting (aligned to +/- strand): ref base C (19/22); new base T (6/10); total (25/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCCTTCTTTAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCTGGAAGCCGGTTACAAAGC > W3110S.gb/3018788‑3018922 | tGCCTTCTTTAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGATTAACg > 1:3845165/1‑71 (MQ=255)tGCCTTCTTTAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGATTAACg > 1:228362/1‑71 (MQ=255) cttcttTAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGt > 1:1097655/1‑70 (MQ=255) cttTAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTaa < 1:4144940/69‑1 (MQ=255) tttAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACg > 1:3618630/1‑70 (MQ=255) tttAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACg > 1:2610348/1‑70 (MQ=255) aaaCATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACt > 1:4768238/1‑71 (MQ=255) aaCATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGaa > 1:4838493/1‑68 (MQ=255) aaCATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACt < 1:2755191/70‑1 (MQ=255) cATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACtgat < 1:2905836/71‑1 (MQ=255) cGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGATTAACGGTAACGAACTGATGAGCCGCaa < 1:1434145/70‑1 (MQ=255) ggtggtCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACtt > 1:2076253/1‑71 (MQ=255) ggtggtCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACtt > 1:105339/1‑71 (MQ=255) gtggtCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACttt < 1:4469661/71‑1 (MQ=255) tggtCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTg < 1:334725/71‑1 (MQ=255) gtCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAAc < 1:2190220/41‑1 (MQ=255) tCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGATTaa < 1:1561752/39‑1 (MQ=255) tCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGATTaa < 1:1463439/39‑1 (MQ=255) ccGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACtga > 1:3960875/1‑52 (MQ=255) ccGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACtga > 1:304239/1‑52 (MQ=255) cGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACg < 1:2329325/45‑1 (MQ=255) cGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCATCTTTGgcgc > 1:1782115/1‑70 (MQ=255) cGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGgcgc > 1:4646618/1‑70 (MQ=255) cGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGgcgc > 1:3103423/1‑70 (MQ=255) gCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGa < 1:4617280/71‑1 (MQ=255) cTGTGGCCTGACAGTTTTGCCAAAGCCCTGATTAACGGTAACGa < 1:880134/44‑1 (MQ=255) gtgGCCTGACAGTTTTGCCAAAGCCCTGATTAACGGTAACGAAc > 1:4624166/1‑44 (MQ=255) gtgGCCTGACAGTTTTGCCAAAGCCCTGATTAACGGTAACGAAc > 1:1732153/1‑44 (MQ=255) tgGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAAt < 1:4724382/70‑1 (MQ=255) gCCTGACAGTTTTGCCAAAGCCCTGACTAACTGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTg < 1:3386002/71‑1 (MQ=255) gCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAAttt < 1:4977999/70‑1 (MQ=255) cTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACg < 1:829891/36‑1 (MQ=255) gACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTg < 1:1870559/55‑1 (MQ=255) gACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTg < 1:1348996/55‑1 (MQ=255) aCAGTTTTGCCAAAGCCCTGATTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCtt > 1:3599186/1‑71 (MQ=255) aCAGTTTTGCCAAAGCCCTGATTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCt < 1:134653/70‑1 (MQ=255) aCAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCtt < 1:564749/71‑1 (MQ=255) aCAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCtt < 1:509010/71‑1 (MQ=255) aCAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCtt < 1:3353505/71‑1 (MQ=255) aCAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCtt < 1:1488088/71‑1 (MQ=255) aGTTTTGCCAAAGCCCTGATTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCt > 1:4548158/1‑71 (MQ=255) aGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCt > 1:4972160/1‑71 (MQ=255) aGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCt > 1:485071/1‑71 (MQ=255) ttttGCCAAAGCCCTGATTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGaaa < 1:1316575/58‑1 (MQ=255) ttttGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTc < 1:4721406/68‑1 (MQ=255) ttGCCAAAGCCCTGATTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGaaa < 1:455739/56‑1 (MQ=255) ttGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAAttt > 1:4628143/1‑59 (MQ=255) ttGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAAttt > 1:2995925/1‑59 (MQ=255) tGCCAAAGCCCTGACTAACGCTAACGAACTGATGAGCCGCAACTTTGGCGCGaaa < 1:4554571/55‑1 (MQ=255) ccAAAGCCCTGATTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCTGGAAGc < 1:1983219/70‑1 (MQ=255) aaGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCTGGAAGCCGGt < 1:694190/71‑1 (MQ=255) aGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAAttt > 1:4284055/1‑52 (MQ=255) aGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAAttt > 1:706077/1‑52 (MQ=255) gCCCTGATTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCTGGAAGCCGGTTa < 1:4177414/71‑1 (MQ=255) ccTGATTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCTGGAAGCCGGTTACa < 1:3318998/71‑1 (MQ=255) aCTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCTGTAAGCCGGTTATAAAg < 1:3184876/70‑1 (MQ=255) aCTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCTGGAAGCCGGTTACAAAGc > 1:4721312/1‑71 (MQ=255) | TGCCTTCTTTAAACATCGCGATGCGGGTGGTCCGCTGTGGCCTGACAGTTTTGCCAAAGCCCTGACTAACGGTAACGAACTGATGAGCCGCAACTTTGGCGCGAAATTTGGGCTTCTGGAAGCCGGTTACAAAGC > W3110S.gb/3018788‑3018922 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |