| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,029,347 | G→A | 8.8% | Q81* (CAA→TAA) | ygfT ← | fused predicted oxidoreductase Fe‑S subunit and nucleotide‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,029,347 | 0 | G | A | 8.8% | 322.4 / 15.6 | 102 | Q81* (CAA→TAA) | ygfT | fused predicted oxidoreductase Fe‑S subunit and nucleotide‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base G (43/50); new base A (5/4); total (48/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01 | |||||||||||
Only 100 of 107 total aligned reads displayed.
TCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTG > W3110S.gb/3029278‑3029416 | tctcAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTg > 1:3225475/1‑70 (MQ=255) tcAACTACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGCCCAGTTGTAc > 1:4975298/1‑71 (MQ=255) tcAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTAc > 1:120336/1‑71 (MQ=255) tcAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTAc > 1:1374972/1‑71 (MQ=255) tcAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTAc > 1:4061/1‑71 (MQ=255) aaCGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTa < 1:1871672/68‑1 (MQ=255) aaCGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGc > 1:5023964/1‑71 (MQ=255) acgacgCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCt < 1:3722187/71‑1 (MQ=255) acgCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATc > 1:4159676/1‑71 (MQ=255) ccAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTg < 1:2431404/59‑1 (MQ=255) aaaGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCg < 1:2727577/67‑1 (MQ=255) aaaGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACt > 1:2167441/1‑71 (MQ=255) cAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTATACGCTATCGGACTGGAAAGt > 1:4566915/1‑71 (MQ=255) gCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTATACGCt < 1:1071287/53‑1 (MQ=255) cGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCa > 1:3810416/1‑69 (MQ=255) cGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCa > 1:3536935/1‑69 (MQ=255) gATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTAt > 1:3363510/1‑53 (MQ=255) ttGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCa < 1:2945639/71‑1 (MQ=255) tGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTa > 1:1608764/1‑44 (MQ=255) tGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTa > 1:2604524/1‑44 (MQ=255) tGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCAt > 1:4450632/1‑71 (MQ=255) gcgcATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGa < 1:4341223/53‑1 (MQ=255) gcgcATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGa < 1:3163313/53‑1 (MQ=255) gcgcATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGa < 1:3693540/53‑1 (MQ=255) gcgcATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCAt < 1:122319/70‑1 (MQ=255) gcgcATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCAt < 1:574177/70‑1 (MQ=255) cgcATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTa < 1:1093468/71‑1 (MQ=255) cATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGa < 1:3607144/55‑1 (MQ=255) cATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGa < 1:724100/55‑1 (MQ=255) aTCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCag < 1:4404876/61‑1 (MQ=255) tCTTTTACAACCAATACATTTTTGCTCGTCCAGTTg < 1:3317541/36‑1 (MQ=255) tCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTa < 1:4683556/43‑1 (MQ=255) tCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTa < 1:2467850/43‑1 (MQ=255) tCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTa < 1:3214872/43‑1 (MQ=255) ttttACAACCAATACATTTTTGCTCGTCCAGTTGTa > 1:1010891/1‑36 (MQ=255) ttttACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGa > 1:3595508/1‑71 (MQ=255) ttttACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGa > 1:474653/1‑71 (MQ=255) tttACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTa > 1:355946/1‑40 (MQ=255) tttACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTa > 1:4834381/1‑40 (MQ=255) tttACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGa > 1:2015669/1‑70 (MQ=255) ttACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGAc < 1:2308551/70‑1 (MQ=255) ttACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGAc > 1:2155860/1‑70 (MQ=255) aCAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAg > 1:4505798/1‑71 (MQ=255) cAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGAc < 1:1949454/67‑1 (MQ=255) cAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGc < 1:3789185/71‑1 (MQ=255) aCCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAAc > 1:2849805/1‑60 (MQ=255) aCCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACa < 1:4178016/66‑1 (MQ=255) aCCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACa < 1:1836420/66‑1 (MQ=255) ccAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGt < 1:2325110/71‑1 (MQ=255) ccAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGt < 1:4902529/71‑1 (MQ=255) aaTACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTg > 1:2964064/1‑39 (MQ=255) aaTACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAAc > 1:2632472/1‑57 (MQ=255) aaTACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTaa > 1:130906/1‑71 (MQ=255) aaTACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCAGTaa > 1:2336759/1‑71 (MQ=255) aTACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTa < 1:3314992/54‑1 (MQ=255) tACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGt < 1:1590583/67‑1 (MQ=255) tACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGt < 1:2873810/67‑1 (MQ=255) aCATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACg > 1:4730385/1‑70 (MQ=255) aCATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACg > 1:1839478/1‑70 (MQ=255) aCATTTTTGCTCGTCCAGTTATACGCTATCGGACTgg < 1:3506046/37‑1 (MQ=255) cATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGt < 1:3424692/41‑1 (MQ=255) cATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCa > 1:431473/1‑48 (MQ=255) cATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCa > 1:3711662/1‑48 (MQ=255) cATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCa > 1:3739431/1‑48 (MQ=255) cATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTaa > 1:3266124/1‑52 (MQ=255) cATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTaa > 1:861878/1‑67 (MQ=255) cATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTaa > 1:1744211/1‑67 (MQ=255) cATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTaa > 1:1641520/1‑67 (MQ=255) cATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGc > 1:4166983/1‑70 (MQ=255) aTTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCTGACAAGCCGTAACGc > 1:5037887/1‑69 (MQ=255) tttttGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGt < 1:2009689/39‑1 (MQ=255) tttGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTc < 1:2049574/38‑1 (MQ=255) tttGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAAc > 1:2798704/1‑49 (MQ=255) tttGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAg > 1:1296853/1‑69 (MQ=255) tttGCTCGTCCAGTTATACGCTATCGGACTGGaaa > 1:5032320/1‑35 (MQ=255) tttGCTCGTCCAGTTATACGCTATCGGACTGGaaa > 1:2578544/1‑35 (MQ=255) tGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAgggg < 1:3665354/70‑1 (MQ=255) tGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAgggg < 1:1869122/70‑1 (MQ=255) tGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAgggg < 1:1293185/70‑1 (MQ=255) cTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCAt < 1:3535739/71‑1 (MQ=255) cTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCAt < 1:3573669/71‑1 (MQ=255) tCCAGTTATACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACg < 1:1313938/57‑1 (MQ=255) tCCAGTTATACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACg < 1:1308044/57‑1 (MQ=255) cAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCg > 1:4262799/1‑41 (MQ=255) cAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCAttg > 1:558215/1‑67 (MQ=255) cAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGc < 1:2960387/71‑1 (MQ=255) cAGTTATACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGc > 1:2433229/1‑56 (MQ=255) aGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCa < 1:1451305/71‑1 (MQ=255) gTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACa < 1:2415640/43‑1 (MQ=255) gTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACa < 1:3682645/43‑1 (MQ=255) gTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACa < 1:882573/43‑1 (MQ=255) gTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCAttgt < 1:3369606/66‑1 (MQ=255) ttGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCAt < 1:3137088/62‑1 (MQ=255) ttGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGt < 1:4235817/71‑1 (MQ=255) ttGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGt < 1:3209792/71‑1 (MQ=255) ttGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGt < 1:5057547/71‑1 (MQ=255) ttGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGt < 1:3838275/71‑1 (MQ=255) ttGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGt < 1:3223623/71‑1 (MQ=255) ttGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGt < 1:340401/71‑1 (MQ=255) gTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTg < 1:2991074/70‑1 (MQ=255) | TCTCAACGACGCCAAAGGGGCAAGCGATTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTG > W3110S.gb/3029278‑3029416 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |