Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,055,306 C→T 10.2% T137I (ACC→ATC)  ygfA → predicted ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,055,3060CT10.2% 113.4 / 4.1 39T137I (ACC→ATC) ygfApredicted ligase
Reads supporting (aligned to +/- strand):  ref base C (13/22);  new base T (2/2);  total (15/24)
Fisher's exact test for biased strand distribution p-value = 6.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

TCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGAAAAA  >  W3110S.gb/3055237‑3055376
                                                                     |                                                                      
tCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGAcc                                                                       >  1:5045229/1‑71 (MQ=255)
 cacaCCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGAcc                                                                       >  1:692119/1‑70 (MQ=255)
 cacaCCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGAcc                                                                       >  1:1193670/1‑70 (MQ=255)
 cacaCCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGAcc                                                                       >  1:3101474/1‑70 (MQ=255)
 cacaCCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGAcc                                                                       >  1:2842001/1‑70 (MQ=255)
 cacaCCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCt                                                                      >  1:3295744/1‑71 (MQ=255)
  acacCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCtt                                                                     <  1:194999/71‑1 (MQ=255)
      cGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACa                                                                  >  1:3300207/1‑70 (MQ=255)
        cTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACaaaa                                                               >  1:1090252/1‑71 (MQ=255)
          ggTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTTGTTTTTATGATCGGACCTTACAAAACt                                                             >  1:4688161/1‑71 (MQ=255)
                 tttGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGATCTTACAAAGCTGgcagca                                                      >  1:1099044/1‑71 (MQ=255)
                 tttGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGATCTTACAAAACTGgcagca                                                      >  1:1205460/1‑71 (MQ=255)
                 tttGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACaa                                                                 <  1:2814617/60‑1 (MQ=255)
                 tttGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACaa                                                                 <  1:2108934/60‑1 (MQ=255)
                  ttGATGAGTGCGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCAc                                                     >  1:810712/1‑71 (MQ=255)
                    gatgaGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACta                                                   >  1:2617388/1‑71 (MQ=255)
                      tgaGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACtata                                                 <  1:3973255/71‑1 (MQ=255)
                      tgaGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACtata                                                 <  1:3967341/71‑1 (MQ=255)
                            cGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACg                                            >  1:1133055/1‑70 (MQ=255)
                            cGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACg                                            >  1:4264943/1‑70 (MQ=255)
                            cGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGc                                           <  1:4543858/71‑1 (MQ=255)
                             ggTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCa                                          <  1:2321606/71‑1 (MQ=255)
                                 aGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGATCTTACAAAACTGGCAGCACTATAAAACGCAAcc                                       <  1:1340528/70‑1 (MQ=255)
                                      cTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGgcagc                                                       <  1:3340213/49‑1 (MQ=255)
                                         ggAATGGGCGGTGGTTTTTATGATCGGATCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGt                                <  1:3781431/69‑1 (MQ=255)
                                         ggAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGgcagca                                                      >  1:4614847/1‑47 (MQ=255)
                                         ggAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGgcagca                                                      >  1:1992353/1‑47 (MQ=255)
                                          gAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAAc                                                              >  1:916014/1‑38 (MQ=255)
                                          gAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAAc                                                              >  1:4181057/1‑38 (MQ=255)
                                          gAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTAt                             >  1:171155/1‑71 (MQ=255)
                                                 cGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATg                      <  1:257330/71‑1 (MQ=255)
                                                  ggtggtTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGa                     <  1:3368686/71‑1 (MQ=255)
                                                  ggtggtTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGa                     <  1:834730/71‑1 (MQ=255)
                                                   gtggtTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGa                     <  1:1302218/70‑1 (MQ=255)
                                                       tttttATGATCTGACCTTACAAAACTGGCAGCACTATAAAACGc                                           <  1:2392904/44‑1 (MQ=255)
                                                       tttttATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGAtt                   <  1:4340913/68‑1 (MQ=255)
                                                         tttATGATCGGACCTTACAAAACTGGCAGCACTATaaa                                               <  1:2656927/38‑1 (MQ=255)
                                                           tATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGt             <  1:3530652/70‑1 (MQ=255)
                                                             tGATCGGACCTTACAAAACTGGCAGCACTATAAAACGc                                           <  1:4305598/38‑1 (MQ=255)
                                                             tGATCGGACCTTACAAAACTGGCAGCACTATAAAACGc                                           <  1:362527/38‑1 (MQ=255)
                                                             tGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTtgg          <  1:852189/71‑1 (MQ=255)
                                                                   gACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGaaa    <  1:2745137/71‑1 (MQ=255)
                                                                   gACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGaaa    <  1:2713554/71‑1 (MQ=255)
                                                                   gACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGaaa    <  1:30306/71‑1 (MQ=255)
                                                                     ccTTACAAAACTGGCAGCACTATAAAACGCAACCgg                                     <  1:4249986/36‑1 (MQ=255)
                                                                     ccTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGaaaaa  <  1:1460446/71‑1 (MQ=255)
                                                                     ccTTACAAAACTGGCAGCACTATAAAACCCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGaaaaa  <  1:685130/71‑1 (MQ=255)
                                                                     |                                                                      
TCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGAAAAA  >  W3110S.gb/3055237‑3055376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: