| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,065,341 | G→T | 38.7% | I163I (ATC→ATA) | ygfI ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,065,341 | 0 | G | T | 38.7% | 95.8 / 53.7 | 62 | I163I (ATC→ATA) | ygfI | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (21/17); new base T (15/9); total (36/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGATAATAAAATCTAAC > W3110S.gb/3065272‑3065407 | ctcttgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa < 1:3111293/71‑1 (MQ=255) tgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGt > 1:1390218/1‑71 (MQ=255) tgctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGt > 1:4314267/1‑71 (MQ=255) gctcttgTCGCTCTTCATAACGGCGATAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTTATAGt > 1:790438/1‑70 (MQ=255) gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTtata < 1:1462132/68‑1 (MQ=255) gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTTATAGt < 1:877697/70‑1 (MQ=255) gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTTATAGt > 1:2007472/1‑70 (MQ=255) gctcttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTTATAGt > 1:4454670/1‑70 (MQ=255) ttgTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTTATAGTCAg < 1:2226425/69‑1 (MQ=255) gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTTATAGTCAg > 1:3043329/1‑67 (MQ=255) gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGttt > 1:2645369/1‑71 (MQ=255) gCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTTATAGTCAGGTTTTc > 1:4358246/1‑70 (MQ=255) gCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTc < 1:3413053/70‑1 (MQ=255) ttCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTTATAGTCAGGTTTTCCAg < 1:615341/69‑1 (MQ=255) tCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTTATAg < 1:617716/55‑1 (MQ=255) tCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGtt > 1:4637804/1‑70 (MQ=255) tCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTc > 1:3990896/1‑71 (MQ=255) cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTTATAg < 1:1675802/54‑1 (MQ=255) cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTTATAg < 1:1669902/54‑1 (MQ=255) aaCGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTTATAGTCAGGTTTTCCAGTTCCTg > 1:4204939/1‑70 (MQ=255) aaCGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTTATAGTCAGGTTTTCCAGTTCCTg > 1:3542750/1‑70 (MQ=255) aCGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGaa > 1:251804/1‑71 (MQ=255) aCGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGaa < 1:1110611/71‑1 (MQ=255) cGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAAc < 1:4528322/71‑1 (MQ=255) cgagaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTTATAGTCAGGTTTTCCAGTTCCTGAACTCg < 1:4414084/71‑1 (MQ=255) cgagaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTTATAGTCAGGTTTTCCAGTTCCTGAACTCg < 1:810570/71‑1 (MQ=255) gaaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTg > 1:3566019/1‑70 (MQ=255) aaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCa < 1:3586965/71‑1 (MQ=255) aaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCa < 1:486921/71‑1 (MQ=255) aaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCa < 1:3209333/71‑1 (MQ=255) aaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCa < 1:2981661/71‑1 (MQ=255) aacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAg < 1:2323667/71‑1 (MQ=255) acgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTg > 1:4165088/1‑67 (MQ=255) cgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTTATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGa > 1:1522040/1‑70 (MQ=255) gagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTcc > 1:5002840/1‑49 (MQ=255) gagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTcc > 1:387481/1‑49 (MQ=255) gagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTcc > 1:2773998/1‑49 (MQ=255) gagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATa > 1:2635893/1‑71 (MQ=255) agaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATa > 1:4668131/1‑70 (MQ=255) gaaaGCCTAATCGCAGCGGTGGTAGTTCACTTATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACa > 1:1776160/1‑71 (MQ=255) gCCTAATCTCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCt > 1:4631960/1‑71 (MQ=255) ccTAATCGCAGCGGTGGTAGTTCACTTATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCtg > 1:2313324/1‑71 (MQ=255) ccTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCtg < 1:4497840/71‑1 (MQ=255) cTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCtgt > 1:2681399/1‑71 (MQ=255) aaTCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGc > 1:4541392/1‑56 (MQ=255) tCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCtgtg < 1:1070543/68‑1 (MQ=255) gCAGCGGTGGTAGTTCACTTATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATg > 1:390043/1‑61 (MQ=255) gCAGCGGTGGTAGTTCACTTATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATg > 1:2831926/1‑61 (MQ=255) gCAGCGGTGGTAGTTCACTTATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTggg > 1:1280783/1‑68 (MQ=255) gCAGCGGTGGTAGTTCACTTATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTggg > 1:675746/1‑68 (MQ=255) gCAGCGGTGGTAGTTCACTTATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTggg > 1:3747744/1‑68 (MQ=255) gCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACa > 1:4026059/1‑59 (MQ=255) gCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCa < 1:931000/50‑1 (MQ=255) gtggtAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATa < 1:2763387/51‑1 (MQ=255) gtggtAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATa < 1:5065338/51‑1 (MQ=255) gtAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGa > 1:5033988/1‑46 (MQ=255) gtAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGATAATaaa < 1:3089536/70‑1 (MQ=255) ttCACTTATAGTCAGGTTTTCCAGTTCCTTAACTCGTGCAGATACATGCTGTGGGGAGATAATaaa < 1:4516027/66‑1 (MQ=255) cACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGa > 1:2484185/1‑40 (MQ=255) cACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGATAATAAAATCTAAc > 1:5007124/1‑71 (MQ=255) cACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGATAATAAAATCTAAc > 1:2354077/1‑71 (MQ=255) aCTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGATAATaaaa < 1:1733570/64‑1 (MQ=255) aCTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGATAATaaaa < 1:2849075/64‑1 (MQ=255) gATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATa < 1:4944029/38‑1 (MQ=255) gATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATa < 1:275600/38‑1 (MQ=255) gATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATa < 1:3783261/38‑1 (MQ=255) | CTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCAGATACATGCTGTGGGGAGATAATAAAATCTAAC > W3110S.gb/3065272‑3065407 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |