Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,088,289 T→C 8.6% N450N (AAT→AAC)  galP → D‑galactose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,088,2890TC 8.6% 194.3 / 9.4 70N450N (AAT→AAC) galPD‑galactose transporter
Reads supporting (aligned to +/- strand):  ref base T (31/33);  new base C (3/3);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

TTTATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAAGCTTCCTCCCATCG  >  W3110S.gb/3088221‑3088357
                                                                    |                                                                    
tttATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCt                                                                    <  1:4335920/71‑1 (MQ=255)
tttATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCt                                                                    <  1:3075079/71‑1 (MQ=255)
tttATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCt                                                                    <  1:25205/71‑1 (MQ=255)
 ttATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCt                                                                    >  1:1327931/1‑70 (MQ=255)
  tATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCtga                                                                  >  1:4352128/1‑71 (MQ=255)
  tATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCt                                                                    <  1:4602459/69‑1 (MQ=255)
      ctgctgACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCtgatga                                                               >  1:1403904/1‑70 (MQ=255)
      ctgctgACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCtgatga                                                               <  1:2911303/70‑1 (MQ=255)
      ctgctgACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGaa                                                              <  1:102469/71‑1 (MQ=255)
      ctgctgACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGaa                                                              <  1:4962546/71‑1 (MQ=255)
      ctgctgACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGaa                                                              <  1:2025189/71‑1 (MQ=255)
      ctgctgACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGaa                                                              <  1:1952454/71‑1 (MQ=255)
        gctgACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAAt                                                                      >  1:3197239/1‑61 (MQ=255)
        gctgACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAAt                                                                      >  1:3579441/1‑61 (MQ=255)
        gctgACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAAt                                                                      >  1:5037220/1‑61 (MQ=255)
        gctgACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAAt                                                                      >  1:4729487/1‑61 (MQ=255)
        gctgACATTGTGGCTGGTACCGGAAACCAAACACGTTTAGCTGGAACATATTGGACGTAATCTGATGAAAg                                                            >  1:4661905/1‑71 (MQ=255)
         ctgACATTGTGGCTGGTACAGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAg                                                            <  1:3045401/70‑1 (MQ=255)
          tgACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGt                                                          >  1:3166831/1‑71 (MQ=255)
               ttGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTaa                                                     <  1:1185013/71‑1 (MQ=255)
                  tgGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAAc                                                   <  1:322024/70‑1 (MQ=255)
                  tgGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAAc                                                   <  1:2138724/70‑1 (MQ=255)
                  tgGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAAc                                                   <  1:1797351/70‑1 (MQ=255)
                   ggCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCt                                                                    <  1:1719199/52‑1 (MQ=255)
                       ggTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAgg                                                           >  1:1008274/1‑57 (MQ=255)
                        gTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCtga                                                                  >  1:4466611/1‑49 (MQ=255)
                        gTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGa                                            <  1:4705981/71‑1 (MQ=255)
                        gTACCGGAAAACAAACACGTTTCGCTGGAACATATTGAACGTAATCtgatga                                                               >  1:3268773/1‑52 (MQ=255)
                         tACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCtgatga                                                               <  1:696693/51‑1 (MQ=255)
                         tACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTaa                                                     <  1:2075363/61‑1 (MQ=255)
                         tACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCTCGaa                                           <  1:4358406/71‑1 (MQ=255)
                           ccGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGaaa                                                             >  1:3749/1‑51 (MQ=255)
                           ccGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGaaa                                                             >  1:1324154/1‑51 (MQ=255)
                           ccGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGaaa                                          <  1:4510279/70‑1 (MQ=255)
                           ccGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGaaa                                          >  1:4681751/1‑70 (MQ=255)
                           ccGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGaaa                                          <  1:58864/70‑1 (MQ=255)
                           ccGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAAt                                         <  1:1032865/71‑1 (MQ=255)
                           ccGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAAt                                         <  1:1599191/71‑1 (MQ=255)
                            cGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTTAACTGCGCGAAATa                                        <  1:3604825/71‑1 (MQ=255)
                            cGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATa                                        <  1:3269065/71‑1 (MQ=255)
                              gAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAgg                                      >  1:4629653/1‑71 (MQ=255)
                                  ccAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCt                                  >  1:1598267/1‑71 (MQ=255)
                                  ccAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCt                                  >  1:423912/1‑71 (MQ=255)
                                   cAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAAt                                         <  1:4175259/63‑1 (MQ=255)
                                     aaCACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCAc                               >  1:2864682/1‑71 (MQ=255)
                                     aaCACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCAc                               <  1:4351876/71‑1 (MQ=255)
                                     aaCACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCAc                               <  1:1889923/71‑1 (MQ=255)
                                      acacGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACg                              <  1:2291017/71‑1 (MQ=255)
                                       cacGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGa                             >  1:2495745/1‑71 (MQ=255)
                                            ttCGCTGTAACATATTGAACGTAACCTGATGAAAGGTCGTAAACTGCGCGaaa                                          <  1:2855270/53‑1 (MQ=38)
                                            ttCGCTGGAACATATTGAACGTAACCTGATGAAAGGTCGTAAACTGCGCGaaa                                          <  1:4535215/53‑1 (MQ=255)
                                            ttCGCTGGAACATATTGAACGTAACCTGATGAAAGGTCGTAAACTGCGCGaaa                                          <  1:1952906/53‑1 (MQ=255)
                                             tCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAAtc                       >  1:2857192/1‑71 (MQ=255)
                                             tCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAAtc                       >  1:4380887/1‑71 (MQ=255)
                                                 tGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTccc                   >  1:2771548/1‑71 (MQ=255)
                                                   gAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATa                                        >  1:2314866/1‑48 (MQ=255)
                                                   gAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCAc                               <  1:2101470/57‑1 (MQ=255)
                                                   gAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTcccc                  <  1:52482/70‑1 (MQ=255)
                                                   gAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTcccc                  >  1:4401436/1‑70 (MQ=255)
                                                   gAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTccc                   >  1:2001812/1‑69 (MQ=255)
                                                        tatTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTcccc                  <  1:1549352/65‑1 (MQ=255)
                                                        tatTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCa                 <  1:2810572/66‑1 (MQ=255)
                                                        tatTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAAGCtt            >  1:4107024/1‑71 (MQ=255)
                                                         atTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAAGCTtc           >  1:3675583/1‑71 (MQ=255)
                                                          ttGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAAGCTtcc          >  1:2237486/1‑71 (MQ=255)
                                                          ttGAACGTAATCTGATGAAAGGTAGTAAACTGCGCGAAATa                                        >  1:3239287/1‑41 (MQ=255)
                                                           tGAACGTAACCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGa                             >  1:2018165/1‑51 (MQ=255)
                                                           tGAACGTAACCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGa                             >  1:1247752/1‑51 (MQ=255)
                                                            gAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATa                                        >  1:1932478/1‑39 (MQ=255)
                                                            gAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAAGCTtcctc        >  1:493963/1‑71 (MQ=255)
                                                            gAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAAGCTtcctc        >  1:2153917/1‑71 (MQ=255)
                                                            gAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAAGCTtcctc        >  1:633958/1‑71 (MQ=255)
                                                            gAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAAGCTtcctc        >  1:1806214/1‑71 (MQ=255)
                                                            gAACGTAACCTGATGAAAGGTCGTAAACTGCGCGAAATa                                        >  1:4876015/1‑39 (MQ=255)
                                                                    tCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAAGCTTCCTCCCATcg  <  1:3365362/69‑1 (MQ=255)
                                                                    |                                                                    
TTTATCCTGCTGACATTGTGGCTGGTACCGGAAACCAAACACGTTTCGCTGGAACATATTGAACGTAATCTGATGAAAGGTCGTAAACTGCGCGAAATAGGCGCTCACGATTAATCTCCCCAAGCTTCCTCCCATCG  >  W3110S.gb/3088221‑3088357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑