| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,113,649 | C→T | 50.8% | A1374T (GCA→ACA) | yghJ ← | predicted inner membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,113,649 | 0 | C | T | 50.8% | ‑6.0 / 92.4 | 61 | A1374T (GCA→ACA) | yghJ | predicted inner membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base C (11/19); new base T (18/13); total (29/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGCTCTCCTCCAGATA > W3110S.gb/3113583‑3113715 | gaggagTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGTGTAc < 1:1338604/71‑1 (MQ=255) gTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATcag < 1:1441227/71‑1 (MQ=255) tCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATca < 1:3920150/64‑1 (MQ=255) cTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGTGTACATCAGCAGa > 1:1018961/1‑68 (MQ=255) tACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGTGTACATCAGCAGACGGTCAcc > 1:4658901/1‑71 (MQ=255) cATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGTGTa > 1:2316112/1‑50 (MQ=255) cATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGTTCAccc < 1:2594470/69‑1 (MQ=255) cATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCAccc < 1:1066318/69‑1 (MQ=255) aTTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGTGTACa < 1:4076032/51‑1 (MQ=255) aTTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGTGTACa < 1:3841473/51‑1 (MQ=255) aTTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGTGTACATcagca < 1:1296964/57‑1 (MQ=255) aTTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGTGTACATcagca < 1:2274669/57‑1 (MQ=255) tttCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGc > 1:4197645/1‑45 (MQ=255) tttCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGTGTACATCAGCAGACGGTCACCCGCAc > 1:3899568/1‑71 (MQ=255) tttCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTa > 1:954645/1‑48 (MQ=255) tttCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGc > 1:2374764/1‑69 (MQ=255) ttCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGt < 1:5136011/61‑1 (MQ=255) ttCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGt < 1:5116878/61‑1 (MQ=255) tCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACgg > 1:625294/1‑59 (MQ=255) cTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGTGTACATCAGCAGACGGTCACCCGCAccgc > 1:4713368/1‑71 (MQ=255) tggtatCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGTGTACATCAGCAGACGGTCACCCGc > 1:2201521/4‑64 (MQ=255) tGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGTGTACATCAGCAGACGGTCACCCGc > 1:4594576/1‑64 (MQ=255) tGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCAccgccg > 1:634993/1‑71 (MQ=255) tGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCAccgccg > 1:1434655/1‑71 (MQ=255) cAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTa < 1:2249871/37‑1 (MQ=255) cAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTa < 1:160993/37‑1 (MQ=255) aaaGTTTTTCTCTGCCCATTCCTTCAGCTGTGTGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGcc < 1:4859070/70‑1 (MQ=255) aaaGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGcc < 1:4598847/70‑1 (MQ=255) aaaGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGcc < 1:2591422/70‑1 (MQ=255) aGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCAt < 1:4105385/71‑1 (MQ=255) gTTTTTCTCTGCCCATTCCTTCAGCTGTGTGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCa > 1:3241625/1‑69 (MQ=255) ttttCTCTGCCCATTCCTTCAGCTGTGTGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGcc > 1:3078424/1‑71 (MQ=255) ttttCTCTGCCCATTCCTTCAGCTGTGTGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGcc > 1:942156/1‑71 (MQ=255) tttCTCTGCCCATTCCTTCAGCTGTGTGTACATca > 1:3015568/1‑35 (MQ=255) tttCTCTGCCCATTCCTTCAGCTGTGTGTACATca > 1:2079422/1‑35 (MQ=255) tttCTCTGCCCATTCCTTCAGCTGTGTGTACATca > 1:1722754/1‑35 (MQ=255) tttCTCTGCCCATTCCTTCAGCTGTGTGTACATca > 1:1514523/1‑35 (MQ=255) tttCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCcgcg > 1:2765914/1‑60 (MQ=255) cccATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGgttgtt < 1:5063957/70‑1 (MQ=255) cccATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGttgttg < 1:2150726/71‑1 (MQ=255) cccATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGttgttg < 1:4116225/71‑1 (MQ=255) aTTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGctc < 1:3057070/71‑1 (MQ=255) ttCCTTCAGCTGTGTGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGcc < 1:3328724/52‑1 (MQ=255) ttCCTTCAGCTGTGTGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGcc < 1:4389951/52‑1 (MQ=255) ttCCTTCAGCTGTGTGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGcc < 1:4347972/52‑1 (MQ=255) ttCCTTCAGCTGTGTGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGcc < 1:1441969/52‑1 (MQ=255) ttCCTTCAGCTGTGTGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGcc < 1:3334768/52‑1 (MQ=255) ttCCTTCAGCTGTGTGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGcc < 1:2561326/52‑1 (MQ=255) ttCCTTCAGCTGTGTGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGcc < 1:2603240/52‑1 (MQ=255) ttCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGctct < 1:1518965/71‑1 (MQ=255) tCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGCtctc > 1:541699/1‑71 (MQ=255) tCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGCtctc > 1:535484/1‑71 (MQ=255) ccTTCGGCTGTGTGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGCTctcc > 1:1052426/1‑71 (MQ=255) ccTTCAGCTGTGTGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGCtctc > 1:2103939/1‑70 (MQ=255) ttCAGCTGTGTGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGCTctcatc > 1:3980445/1‑71 (MQ=255) tCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGttgttg > 1:1731929/1‑62 (MQ=255) cAGCTGTGTGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGCTctcctc > 1:2796688/1‑69 (MQ=255) cAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGCTctcctc < 1:4817160/69‑1 (MQ=255) aGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGCtctc > 1:871450/1‑65 (MQ=255) tgtgCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGACTGGTTGTTGCTCTCCTCCAGata > 1:3930706/1‑71 (MQ=255) tgCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCAt < 1:3793499/43‑1 (MQ=255) tgCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGc < 1:4411864/45‑1 (MQ=255) | GAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGCTCTCCTCCAGATA > W3110S.gb/3113583‑3113715 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |