| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,117,343 | C→A | 10.3% | A142A (GCG→GCT) | yghJ ← | predicted inner membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,117,343 | 0 | C | A | 10.3% | 244.5 / 8.7 | 77 | A142A (GCG→GCT) | yghJ | predicted inner membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base C (32/37); new base A (5/3); total (37/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
CGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGTAACGTCCTCGCCAG > W3110S.gb/3117276‑3117413 | cGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTAcgcg > 1:3132032/1‑69 (MQ=255)cGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTAcgcg > 1:3159561/1‑69 (MQ=255)cGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTAcgcg > 1:1642687/1‑69 (MQ=255)cGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACgcgc < 1:2179527/70‑1 (MQ=255) ggAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCa < 1:2103845/70‑1 (MQ=255) ggAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCa < 1:1929224/70‑1 (MQ=255) aGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGAGCAGCt < 1:1037500/71‑1 (MQ=255) ccGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTct < 1:184178/71‑1 (MQ=255) cAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctgact > 1:1164630/1‑71 (MQ=255) cAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGAGCAGCTTctgact > 1:2681216/1‑71 (MQ=255) cAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGAGCAGCTTctgact > 1:2687247/1‑71 (MQ=255) aGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCtt < 1:2425027/64‑1 (MQ=255) aGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTct < 1:474549/66‑1 (MQ=255) aGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTct < 1:3878672/66‑1 (MQ=255) tGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCTCACGCAAGCTACGCGCAGCTTCTGACTGAGTGtt > 1:129481/1‑71 (MQ=255) gcgcGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGtt < 1:4109102/70‑1 (MQ=255) gcgTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCt < 1:2929296/54‑1 (MQ=255) gcgTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCt < 1:5051886/54‑1 (MQ=255) gTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctga > 1:4286500/1‑57 (MQ=255) gTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAgg < 1:4314689/71‑1 (MQ=255) tCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCt > 1:1838712/1‑51 (MQ=255) cTCAAGGCTAAACGACACTTTTTCAACCGCAGGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTgg > 1:2522042/1‑71 (MQ=255) cTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTgg > 1:1793256/1‑71 (MQ=255) cTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTgg > 1:54626/1‑71 (MQ=255) cTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTgg > 1:3264237/1‑71 (MQ=255) cTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTgg > 1:1310914/1‑71 (MQ=255) aaGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctga > 1:3264057/1‑51 (MQ=255) aaGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCaa > 1:893197/1‑71 (MQ=255) aaGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCa < 1:4002120/70‑1 (MQ=255) aaGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCa < 1:2587953/70‑1 (MQ=255) aGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAAt < 1:2803844/71‑1 (MQ=255) ggCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCtt > 1:3330396/1‑45 (MQ=255) ggCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCtt > 1:962875/1‑45 (MQ=255) ggCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCtt > 1:2117993/1‑45 (MQ=255) ggCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAg > 1:753135/1‑62 (MQ=255) ggCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAg > 1:2175316/1‑62 (MQ=255) ggCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAAtt < 1:4249457/71‑1 (MQ=255) ggCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAAtt < 1:1256413/71‑1 (MQ=255) gCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctgact < 1:4959157/50‑1 (MQ=255) gCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctgact < 1:2336983/50‑1 (MQ=255) cTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTctgact < 1:1307889/49‑1 (MQ=255) aaaCGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCt < 1:4555142/40‑1 (MQ=255) aaaCGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCg > 1:1506207/1‑71 (MQ=255) aaaCGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCg > 1:3113525/1‑71 (MQ=255) aaCGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTc < 1:802082/69‑1 (MQ=255) aaCGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCgt > 1:61702/1‑71 (MQ=255) aaCGACACTTTTTCAACCGCACGCAAGCTACGAGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCg < 1:2203978/70‑1 (MQ=255) aCGACACTTTTTCAACCGCACGCAAGCTACGCGCa < 1:2214393/35‑1 (MQ=255) aCGACACTTTTTCAACCGCACGCAAGCTACGCGCa < 1:1840904/35‑1 (MQ=255) aCGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCgtg < 1:2281087/71‑1 (MQ=255) aCGACACTTTTTCAACCGCACGCAAGCTACGAGCAGCTTctga > 1:750112/1‑43 (MQ=255) cGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCgtgt < 1:2852812/71‑1 (MQ=255) cGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCgtgt < 1:2846967/71‑1 (MQ=255) cGACACTTTTTCAACCGCACGCAAGCTACGAGCAGCTTCTGACTGAGTGTTGAAGGTg > 1:2247250/1‑58 (MQ=255) cGACACTTTTTCAACCGCACGCAAGCTACGAGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCgtgt > 1:3466739/1‑71 (MQ=255) gACACTTTTTAAACCGCACGCAAGCTACGCGCAGCTTctgactga > 1:3780330/1‑45 (MQ=255) cacTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTAc > 1:4766375/1‑71 (MQ=255) cacTTTTTCAACCGCACGAAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTAc > 1:747647/1‑71 (MQ=255) cTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGa < 1:3741215/48‑1 (MQ=255) cTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTAccc > 1:507689/1‑71 (MQ=255) cTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTAccc > 1:4109190/1‑71 (MQ=255) tttttCAACCTCAAGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGa < 1:2388885/47‑1 (MQ=255) tttCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAgg > 1:1452440/1‑48 (MQ=255) tttCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGc > 1:2331076/1‑70 (MQ=255) tttCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGc > 1:3235646/1‑70 (MQ=255) ttCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCa > 1:4730354/1‑71 (MQ=255) ttCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCa > 1:1602396/1‑71 (MQ=255) tCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGcc < 1:4861526/69‑1 (MQ=255) aaCCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGc < 1:2615660/71‑1 (MQ=255) aaCCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGc < 1:2989644/71‑1 (MQ=255) gcacgcaAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGt < 1:468085/71‑1 (MQ=255) gcacgcaAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGt < 1:713555/71‑1 (MQ=255) gcacgcaAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGt < 1:1186060/71‑1 (MQ=255) cacgcaAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCaa > 1:1877180/1‑68 (MQ=255) cacgcaAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCAACGCAAGt > 1:1034187/1‑70 (MQ=255) cacgcaAGCTACGAGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCgtgt < 1:838291/54‑1 (MQ=255) gcaAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGTAACg < 1:4043431/71‑1 (MQ=255) gcaAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGTAACg < 1:3540344/71‑1 (MQ=255) caAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCa < 1:2955072/40‑1 (MQ=255) aaGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGTAACGTc < 1:955001/71‑1 (MQ=255) tACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGTAACGTCCTCg > 1:361286/1‑71 (MQ=255) gcgcAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGTaa > 1:671381/1‑60 (MQ=255) cgcAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGTAACGTCCTCGCCAg > 1:2640220/1‑71 (MQ=255) | CGGAGCCCGCCAGTTCTTGCGCGTCCTCAAGGCTAAACGACACTTTTTCAACCGCACGCAAGCTACGCGCAGCTTCTGACTGAGTGTTGAAGGTGGCAATTGTCGTGTTACCCGCCACGCAAGTAACGTCCTCGCCAG > W3110S.gb/3117276‑3117413 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |