| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,139,938 | C→T | 43.7% | R2H (CGT→CAT) | hybD ← | predicted maturation element for hydrogenase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,139,938 | 0 | C | T | 43.7% | 41.0 / 113.3 | 87 | R2H (CGT→CAT) | hybD | predicted maturation element for hydrogenase 2 |
| Reads supporting (aligned to +/- strand): ref base C (24/25); new base T (17/21); total (41/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
CTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGCACAGGC > W3110S.gb/3139870‑3140004 | cTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACg > 1:1700250/1‑70 (MQ=255) ttCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCa > 1:246736/1‑71 (MQ=255) cAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTa > 1:1129046/1‑69 (MQ=255) aaTCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAg < 1:3181229/71‑1 (MQ=255) ccGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCAt < 1:2958149/63‑1 (MQ=255) ccGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAAc > 1:4812949/1‑71 (MQ=255) cGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGaa > 1:1906411/1‑69 (MQ=255) cGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAAcc > 1:1052741/1‑71 (MQ=255) cacaCCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCtt < 1:4536660/71‑1 (MQ=255) cacaCCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACa > 1:830136/1‑64 (MQ=255) cacaCCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACa > 1:4420595/1‑64 (MQ=255) cacCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCTTAAGACTAAAATACGCATTACAGAACCTTCa > 1:2772014/1‑71 (MQ=255) cacCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCTTAAGACTAAAATACGCATTACAGAACCTTCa > 1:2766006/1‑71 (MQ=255) cacCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCa > 1:3004257/1‑71 (MQ=255) ccGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCtt < 1:3783067/67‑1 (MQ=255) ccGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACt < 1:1758464/71‑1 (MQ=255) cGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACt > 1:1321390/1‑70 (MQ=255) gATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGa < 1:4248945/71‑1 (MQ=255) ggCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAAc > 1:1809202/1‑71 (MQ=255) gCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAAc > 1:3659606/1‑58 (MQ=255) gCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAAc > 1:5083360/1‑58 (MQ=255) tCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCtt < 1:1111959/58‑1 (MQ=255) tCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACt > 1:2377691/1‑71 (MQ=255) cATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCAc < 1:3643531/69‑1 (MQ=255) cATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCAc < 1:200492/69‑1 (MQ=255) cATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCAc < 1:862453/69‑1 (MQ=255) cATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACt < 1:4152441/70‑1 (MQ=255) aTCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTc < 1:2093003/71‑1 (MQ=255) aTCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTc > 1:3840061/1‑71 (MQ=255) aTCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTc > 1:240454/1‑71 (MQ=255) aTCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTc < 1:4747608/71‑1 (MQ=255) tCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTc < 1:3908461/56‑1 (MQ=255) cGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTc < 1:2498966/69‑1 (MQ=255) ggTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCCtt < 1:1928884/71‑1 (MQ=255) gTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGt > 1:1716431/1‑69 (MQ=255) gTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTg < 1:4839980/71‑1 (MQ=255) gTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTg < 1:1762156/71‑1 (MQ=255) tCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTc < 1:1238806/66‑1 (MQ=255) tCAGCAAAATATTGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGc > 1:4392688/1‑71 (MQ=255) cAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTc < 1:1055248/48‑1 (MQ=255) cAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTc < 1:4160151/71‑1 (MQ=255) aaaTATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAg > 1:28548/1‑71 (MQ=255) aaTATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGaa < 1:2708941/51‑1 (MQ=255) aaTATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGc < 1:2737448/71‑1 (MQ=255) ttGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATc < 1:5136027/70‑1 (MQ=255) ttGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATc < 1:658631/70‑1 (MQ=255) ttGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATc < 1:1308103/70‑1 (MQ=255) tGCCGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCa > 1:1484968/1‑71 (MQ=255) ccGACCCCTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCAc < 1:3971536/70‑1 (MQ=255) cccTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACgtg > 1:4211936/1‑71 (MQ=255) cccTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACgtg < 1:95582/71‑1 (MQ=255) cccTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACgtg < 1:3082259/71‑1 (MQ=255) cccTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACgtg < 1:3088000/71‑1 (MQ=255) ccTAAGACTAAAATATGCATTAGAGAACCTTCACTGAAACCACtt < 1:1831543/45‑1 (MQ=255) ccTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACtt < 1:3461341/45‑1 (MQ=255) ccTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACtt < 1:3457451/45‑1 (MQ=255) ccTAAGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACgtg > 1:1107687/1‑70 (MQ=255) cTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCAc < 1:4528869/63‑1 (MQ=255) cTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCAc < 1:2045754/63‑1 (MQ=255) cTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCAc < 1:2250716/63‑1 (MQ=255) cTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACgtgt > 1:4955275/1‑70 (MQ=255) cTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTa > 1:2907734/1‑71 (MQ=255) cTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTACGTTGCCGTCAGCATCCACTACGTGTa > 1:3716396/1‑71 (MQ=255) tAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTc < 1:1304639/44‑1 (MQ=255) tAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAc > 1:350485/1‑71 (MQ=255) tAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAc > 1:1853715/1‑71 (MQ=255) tAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTAc > 1:1285258/1‑71 (MQ=255) aaGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCg > 1:2775337/1‑44 (MQ=255) aaGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACgtgt < 1:4267195/68‑1 (MQ=255) aaGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACgtgt < 1:1038247/68‑1 (MQ=255) aaGACTAAAATATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACgtgt < 1:3240562/68‑1 (MQ=255) aGACTAAAATATGCATTACAGAACCTTCACTGaaa > 1:4171258/1‑35 (MQ=255) aGACTAAAATATGCATTACAGAACCTTCACTGaaa > 1:4769618/1‑35 (MQ=255) aGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTa > 1:4555215/1‑68 (MQ=255) aGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCg < 1:4271242/71‑1 (MQ=255) aGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCg < 1:3272681/71‑1 (MQ=255) gACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCg > 1:3749529/1‑70 (MQ=255) aaaaTATGCATTACAGAACCTTCACTGAAACCACTTCGtt < 1:2659287/40‑1 (MQ=255) aaaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTa > 1:2186374/1‑56 (MQ=255) aaaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGCACAgg > 1:4682143/1‑71 (MQ=255) aaaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGCACAgg > 1:1150492/1‑71 (MQ=255) aaaTATGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCAACTa > 1:1861897/1‑56 (MQ=255) aaaTACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCa > 1:3141086/1‑46 (MQ=255) aaaTACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGCACAgg > 1:1304772/1‑71 (MQ=255) aaTACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGCACAGGc < 1:2381343/71‑1 (MQ=255) aaTACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGCACAGGc < 1:468296/71‑1 (MQ=255) aaTACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGCACAGGc < 1:3338660/71‑1 (MQ=255) | CTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCCGTCAGCATCCACTACGTGTACCGCACAGGC > W3110S.gb/3139870‑3140004 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |