Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 3,191,865 | G→A | 21.3% | A116T (GCC→ACC) | yqiK → | conserved hypothetical protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 3,191,865 | 0 | G | A | 21.3% | 100.6 / 21.4 | 47 | A116T (GCC→ACC) | yqiK | conserved hypothetical protein |
Reads supporting (aligned to +/- strand): ref base G (11/26); new base A (5/5); total (16/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.74e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 |
CGCGTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTC > W3110S.gb/3191799‑3191933 | cgcgTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGcc > 1:1461119/1‑69 (MQ=255) cgcgTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGcc > 1:816048/1‑69 (MQ=255) cgcgTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGcc > 1:3993110/1‑69 (MQ=255) cgcgTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCa < 1:610649/71‑1 (MQ=255) gTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCa > 1:376074/1‑68 (MQ=255) gATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGc < 1:1784926/70‑1 (MQ=255) gATGTAGTAGTAGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGc < 1:675779/70‑1 (MQ=255) gtagtagtaGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGCTggg > 1:3226828/1‑71 (MQ=255) gtagtaGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTgg < 1:1088359/70‑1 (MQ=255) gtagtaGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTgg < 1:3294002/70‑1 (MQ=255) agtagtCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTgggg > 1:4300460/1‑70 (MQ=255) gtagtCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACg > 1:3874450/1‑63 (MQ=255) gtagtCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGc < 1:1981953/70‑1 (MQ=255) gtagtCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGCTGGGGc < 1:3277049/70‑1 (MQ=255) gtagtCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGCTGGGGCa < 1:3868254/71‑1 (MQ=255) tCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGc < 1:2666343/71‑1 (MQ=255) tCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGCTgg < 1:2001825/63‑1 (MQ=255) tctttGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTg > 1:3980518/1‑71 (MQ=255) tctttGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTg > 1:751533/1‑71 (MQ=255) tgtgCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTc < 1:4554505/69‑1 (MQ=255) cGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGCTGGGGCAACGCACCCTGTCGCCTGa < 1:2976699/71‑1 (MQ=255) ggTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGCTGGGGCAAc > 1:967301/1‑53 (MQ=255) aaaCCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACt > 1:389763/1‑70 (MQ=255) aaaCCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACt > 1:3045096/1‑70 (MQ=255) aCCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAAAGCACCCTGTCGCCTGAAGACTTAc < 1:4128900/71‑1 (MQ=255) ccTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACg < 1:3151882/71‑1 (MQ=255) ccTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACg < 1:1141063/71‑1 (MQ=255) cTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACg < 1:2353216/70‑1 (MQ=255) cAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTa < 1:4089068/69‑1 (MQ=255) cAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTa < 1:2935726/69‑1 (MQ=255) cAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTa < 1:1999755/69‑1 (MQ=255) cAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATg > 1:701639/1‑71 (MQ=255) gTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgtt < 1:2944068/71‑1 (MQ=255) gTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgtt < 1:1931254/71‑1 (MQ=255) gAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGAc > 1:2682107/1‑57 (MQ=255) gAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACg < 1:4317106/62‑1 (MQ=255) aGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgttggttg < 1:2109931/71‑1 (MQ=255) gATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgttggttg > 1:208386/1‑68 (MQ=255) aTTGCCACCGCTACCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgt > 1:1648307/1‑61 (MQ=255) ttGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTa < 1:639660/53‑1 (MQ=255) ccACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATaaa < 1:534652/71‑1 (MQ=255) ccGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAAttt < 1:583917/71‑1 (MQ=255) cGCTACCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAAttt < 1:4495171/70‑1 (MQ=255) cTGCCCAGACGCTGGGTCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTc < 1:77642/71‑1 (MQ=255) cTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATg < 1:577088/50‑1 (MQ=255) cTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTc < 1:4569961/71‑1 (MQ=255) cTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTc < 1:3596163/71‑1 (MQ=255) | CGCGTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTC > W3110S.gb/3191799‑3191933 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |