Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,191,865 G→A 21.3% A116T (GCC→ACC)  yqiK → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,191,8650GA21.3% 100.6 / 21.4 47A116T (GCC→ACC) yqiKconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (11/26);  new base A (5/5);  total (16/31)
Fisher's exact test for biased strand distribution p-value = 2.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

CGCGTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTC  >  W3110S.gb/3191799‑3191933
                                                                  |                                                                    
cgcgTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGcc                                                                    >  1:1461119/1‑69 (MQ=255)
cgcgTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGcc                                                                    >  1:816048/1‑69 (MQ=255)
cgcgTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGcc                                                                    >  1:3993110/1‑69 (MQ=255)
cgcgTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCa                                                                  <  1:610649/71‑1 (MQ=255)
   gTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCa                                                                  >  1:376074/1‑68 (MQ=255)
      gATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGc                                                             <  1:1784926/70‑1 (MQ=255)
      gATGTAGTAGTAGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGc                                                             <  1:675779/70‑1 (MQ=255)
         gtagtagtaGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGCTggg                                                         >  1:3226828/1‑71 (MQ=255)
         gtagtaGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTgg                                                          <  1:1088359/70‑1 (MQ=255)
         gtagtaGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTgg                                                          <  1:3294002/70‑1 (MQ=255)
           agtagtCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTgggg                                                        >  1:4300460/1‑70 (MQ=255)
            gtagtCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACg                                                              >  1:3874450/1‑63 (MQ=255)
            gtagtCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGc                                                       <  1:1981953/70‑1 (MQ=255)
            gtagtCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGCTGGGGc                                                       <  1:3277049/70‑1 (MQ=255)
            gtagtCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGCTGGGGCa                                                      <  1:3868254/71‑1 (MQ=255)
                tCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGc                                                  <  1:2666343/71‑1 (MQ=255)
                tCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGCTgg                                                          <  1:2001825/63‑1 (MQ=255)
                      tctttGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTg                                            >  1:3980518/1‑71 (MQ=255)
                      tctttGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTg                                            >  1:751533/1‑71 (MQ=255)
                          tgtgCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTc                                          <  1:4554505/69‑1 (MQ=255)
                              cGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGCTGGGGCAACGCACCCTGTCGCCTGa                                    <  1:2976699/71‑1 (MQ=255)
                                ggTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGCTGGGGCAAc                                                    >  1:967301/1‑53 (MQ=255)
                                    aaaCCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACt                               >  1:389763/1‑70 (MQ=255)
                                    aaaCCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACt                               >  1:3045096/1‑70 (MQ=255)
                                      aCCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAAAGCACCCTGTCGCCTGAAGACTTAc                            <  1:4128900/71‑1 (MQ=255)
                                       ccTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACg                           <  1:3151882/71‑1 (MQ=255)
                                       ccTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACg                           <  1:1141063/71‑1 (MQ=255)
                                        cTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACg                           <  1:2353216/70‑1 (MQ=255)
                                           cAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTa                         <  1:4089068/69‑1 (MQ=255)
                                           cAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTa                         <  1:2935726/69‑1 (MQ=255)
                                           cAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTa                         <  1:1999755/69‑1 (MQ=255)
                                           cAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATg                       >  1:701639/1‑71 (MQ=255)
                                             gTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgtt                     <  1:2944068/71‑1 (MQ=255)
                                             gTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgtt                     <  1:1931254/71‑1 (MQ=255)
                                                gAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGAc                                >  1:2682107/1‑57 (MQ=255)
                                                gAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACg                           <  1:4317106/62‑1 (MQ=255)
                                                  aGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgttggttg                <  1:2109931/71‑1 (MQ=255)
                                                     gATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgttggttg                >  1:208386/1‑68 (MQ=255)
                                                      aTTGCCACCGCTACCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgt                      >  1:1648307/1‑61 (MQ=255)
                                                       ttGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTa                             <  1:639660/53‑1 (MQ=255)
                                                          ccACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATaaa        <  1:534652/71‑1 (MQ=255)
                                                             ccGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAAttt     <  1:583917/71‑1 (MQ=255)
                                                              cGCTACCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAAttt     <  1:4495171/70‑1 (MQ=255)
                                                                cTGCCCAGACGCTGGGTCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTc  <  1:77642/71‑1 (MQ=255)
                                                                cTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATg                       <  1:577088/50‑1 (MQ=255)
                                                                cTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTc  <  1:4569961/71‑1 (MQ=255)
                                                                cTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTc  <  1:3596163/71‑1 (MQ=255)
                                                                  |                                                                    
CGCGTCGATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGTC  >  W3110S.gb/3191799‑3191933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: