| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,205,529 | C→T | 36.4% | D137D (GAC→GAT) | ttdA → | L‑tartrate dehydratase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,205,529 | 0 | C | T | 36.4% | 55.0 / 54.0 | 55 | D137D (GAC→GAT) | ttdA | L‑tartrate dehydratase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (18/17); new base T (8/12); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01 | |||||||||||
GTAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAGGCGGCGGCTGCACGCTACCTG > W3110S.gb/3205461‑3205596 | gTAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGAcat < 1:812903/71‑1 (MQ=255)gTAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGAcat < 1:4583563/71‑1 (MQ=255)gTAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGAcat < 1:1901297/71‑1 (MQ=255) tAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGatat > 1:2242986/1‑70 (MQ=255) tAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGAcatc < 1:4414549/71‑1 (MQ=255) aGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGAcatca > 1:15272/1‑71 (MQ=255) aGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGAGTACCGTGGGTCACCTGGGAcatca > 1:4512772/1‑71 (MQ=255) aTTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATccc > 1:3888068/1‑71 (MQ=255) aTTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATccc > 1:3337916/1‑71 (MQ=255) aCGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAAt < 1:5137036/71‑1 (MQ=255) cGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATg < 1:1136345/71‑1 (MQ=255) aaGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGAc < 1:1756974/71‑1 (MQ=255) aGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGATATCATCCCCGACAATGAc > 1:1011521/1‑70 (MQ=255) gTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGa > 1:4943456/1‑71 (MQ=255) tAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGAcat < 1:3532849/52‑1 (MQ=255) aaaCACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGAc > 1:4304879/1‑67 (MQ=255) aaCACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGc > 1:4519878/1‑71 (MQ=255) acacCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGATATCATCCCCGACAATGACGATGCg > 1:4338686/1‑71 (MQ=255) acCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCgg > 1:4222876/1‑70 (MQ=255) acCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCgg > 1:5051416/1‑70 (MQ=255) acCCGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCgg > 1:5027723/1‑70 (MQ=255) cGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGATATCATCCCCGACAATGACGATGCGGaaa < 1:3252294/71‑1 (MQ=255) aaTACCGGTAGCGGCGTACCGTGGGTCACCTGGGATATCATCCCCGa > 1:3238567/1‑47 (MQ=255) aaTACCGGTAGCGGCGTACCGTGGGTCACCTGGGATATCATCCCCGACAATGACGATGCGGAAATCGAAGt > 1:2046670/1‑71 (MQ=255) aTACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGtt < 1:2609965/71‑1 (MQ=255) aCCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTa > 1:3323404/1‑71 (MQ=255) ccGGTAGCGGCGTACCGTGGGTCACCTGGGATATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTa < 1:3955588/70‑1 (MQ=255) ccGGTAGCGGCGTACCGTGGGTCACCTGGGATATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTa < 1:2981493/70‑1 (MQ=255) ccGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTa > 1:2919799/1‑70 (MQ=255) ccGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTAc < 1:3835093/71‑1 (MQ=255) cGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTa < 1:3648517/69‑1 (MQ=255) gTAGCGGCGTACCGTGGGTCACCTGGGATATCATCCCCGACAATGACGATGCGGaaa > 1:1337376/1‑57 (MQ=255) tAGCGGCGTACCGTGGGTCACCTGGGATATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACAttg > 1:5071003/1‑69 (MQ=255) tAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACa > 1:660248/1‑68 (MQ=255) tAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACa > 1:4593763/1‑68 (MQ=255) gcggcgTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGc < 1:3217897/70‑1 (MQ=255) gcggcgTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCa < 1:3588687/71‑1 (MQ=255) gcggcgTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCa < 1:4327156/71‑1 (MQ=255) gcggcgTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCa < 1:1621175/71‑1 (MQ=255) gcggcgTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCa < 1:1488700/71‑1 (MQ=255) ggcgTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAgg > 1:1258016/1‑71 (MQ=255) gcgTACCGTGGGTCACCTGGGATATCATCCCCGAc < 1:4961040/35‑1 (MQ=255) gcgTACCGTGGGTCACCTGGGATATCATCCCCGAc < 1:257998/35‑1 (MQ=255) gcgTACCGTGGGTCACCTGGGATATCATCCCCGAc < 1:683423/35‑1 (MQ=255) gcgTACCGTGGGTCACCTGGGATATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAggc < 1:3021870/71‑1 (MQ=255) gTACCGTGGGTCACCTGGGATATCATCCCCGACaa > 1:4594016/1‑35 (MQ=255) tACCGTGGGTCACCTGGGACATCATCCCCGACAATGAc < 1:2397160/38‑1 (MQ=255) tACCGTGGGTCACCTGGGACATCATCCCCGACAATGAc < 1:4910949/38‑1 (MQ=255) tACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAggcggc < 1:2584693/71‑1 (MQ=255) ccGTGGGTCACCTGGGATATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTa < 1:1047854/56‑1 (MQ=255) ccGTGGGTCACCTGGGATATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTa < 1:4747775/56‑1 (MQ=255) ccGTGGGTCACCTGGGATATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTa < 1:4421898/56‑1 (MQ=255) ccGTGGGTCACCTGGGATATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTa < 1:3209808/56‑1 (MQ=255) tGGGTCACCTGGGATATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAGGCGGCGGCTg > 1:1373261/1‑71 (MQ=255) tGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCg < 1:2161501/46‑1 (MQ=255) ggTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGaa > 1:3442703/1‑46 (MQ=255) gTCACCTGGGATATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAgg < 1:2706830/59‑1 (MQ=255) cTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGtt < 1:4559152/43‑1 (MQ=255) gggACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACa > 1:3758760/1‑45 (MQ=255) gggACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAGGCGGCGGCTGCACGCTACCt > 1:77203/1‑71 (MQ=255) gggACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAGGCGGCGGCTGCACGATAc > 1:2296214/1‑69 (MQ=255) gACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAGGCGGCGGCTGCACGCTACCTg > 1:1949057/1‑70 (MQ=255) aCATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAggcggcgg < 1:3456980/55‑1 (MQ=255) | GTAGAAATTTTTGACGAAGTAAACACCGGCAAAAATACCGGTAGCGGCGTACCGTGGGTCACCTGGGACATCATCCCCGACAATGACGATGCGGAAATCGAAGTTTACATGGCAGGCGGCGGCTGCACGCTACCTG > W3110S.gb/3205461‑3205596 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |