| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 36,749 | C→T | 36.5% | R364H (CGC→CAC) | caiC ← | predicted crotonobetaine CoA ligase:carnitine CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 36,749 | 0 | C | T | 36.5% | 53.8 / 50.4 | 52 | R364H (CGC→CAC) | caiC | predicted crotonobetaine CoA ligase:carnitine CoA ligase |
| Reads supporting (aligned to +/- strand): ref base C (23/10); new base T (2/17); total (25/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.99e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTT > W3110S.gb/36682‑36818 | tttGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGAt > 1:4155221/1‑71 (MQ=255)tttGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGAt > 1:4822803/1‑71 (MQ=255) tGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGTGa < 1:1325787/68‑1 (MQ=255) tGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGTGa < 1:3631872/68‑1 (MQ=255) tGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGTGAt < 1:4054462/69‑1 (MQ=255) tGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGTGAt < 1:4048731/69‑1 (MQ=255) tGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATtg > 1:3227957/1‑71 (MQ=255) aaGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGTGATtgt < 1:3564702/70‑1 (MQ=255) aGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGTGATtgtg < 1:1616868/70‑1 (MQ=255) tttCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGTGa > 1:3352432/1‑58 (MQ=255) ttCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTcgcg > 1:2503534/1‑71 (MQ=255) cccAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGTGATTGTGATCGTCGCgg < 1:877960/70‑1 (MQ=255) cccAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGTGATTGTGATCGTCGCgg < 1:3130025/70‑1 (MQ=255) cccAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGTGATTGTGATCGTCGCgg < 1:1880695/70‑1 (MQ=255) ccTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTc > 1:690586/1‑71 (MQ=255) tttAATGCAGATTTCACCGATCTCACCAGGCGGGAGCGGGTGATtgtg < 1:3544899/48‑1 (MQ=255) tttAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGTGATtgtg < 1:2722255/48‑1 (MQ=255) tAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGTGATTGTGATCGTCGCGGATCTCCGCTTCGt < 1:338041/69‑1 (MQ=255) tAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGTGATTGTGATCGTCGCGGATCTCCGCTTCGTAg > 1:3988047/1‑71 (MQ=255) tAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGAt > 1:2233156/1‑58 (MQ=255) tAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGAt > 1:3231905/1‑58 (MQ=255) tAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGAt > 1:3292659/1‑58 (MQ=255) tAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAg > 1:241483/1‑71 (MQ=255) tAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAg > 1:5133534/1‑71 (MQ=255) tAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAg > 1:3712601/1‑71 (MQ=255) tAATGCAGATTTAACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAg > 1:1634099/1‑71 (MQ=255) tGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATtgtg < 1:4365989/43‑1 (MQ=255) tGCAGATTTCACCGATCTCACCAGCC‑GGAGCGGGc < 1:4635501/35‑1 (MQ=37) gCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGAtcgt > 1:590981/1‑47 (MQ=255) cAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCaaaa > 1:1700406/1‑71 (MQ=255) aGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAAc > 1:3015578/1‑71 (MQ=255) aGATTTCACCGATCTCACCAGCCGGGAGC‑GGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAAcc < 1:4841889/71‑1 (MQ=255) aTTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGc < 1:3834538/64‑1 (MQ=255) tttCACCGATCTCACCAGCCGGGAGCTGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAAcccc < 1:3508146/71‑1 (MQ=255) ttCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCa > 1:971627/1‑71 (MQ=255) ttCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCa > 1:4916672/1‑71 (MQ=255) tCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCAc > 1:4734701/1‑71 (MQ=255) gATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCaa < 1:4692069/58‑1 (MQ=255) aTCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCg < 1:3603166/51‑1 (MQ=255) aTCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCg < 1:2095866/51‑1 (MQ=255) ctcACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCaa > 1:2371544/1‑71 (MQ=255) cACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATc > 1:2089636/1‑71 (MQ=255) aCCAGCCGGGAGCGGGTGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCa < 1:3525528/67‑1 (MQ=255) aCCAGCCGGGAGCGGGTGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCg < 1:3597137/71‑1 (MQ=255) aCCAGCCGGGAGCGGGTGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCg < 1:1199460/71‑1 (MQ=255) aCCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATc < 1:283918/70‑1 (MQ=255) aCCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACAAATc > 1:4456091/1‑70 (MQ=255) ccAGCCGGGAGCGGGTGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGa < 1:2921833/71‑1 (MQ=255) ccAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGa > 1:4461670/1‑71 (MQ=255) gCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACgg > 1:1405538/1‑71 (MQ=255) ccGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGc < 1:147674/71‑1 (MQ=255) cGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGcc < 1:2414105/71‑1 (MQ=255) aGCGGGTGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGcg < 1:1008507/71‑1 (MQ=255) cGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGtt > 1:2350701/1‑70 (MQ=255) | TTTGAAGATGGTTTTCCCAGGTATGCCTTTAATGCAGATTTCACCGATCTCACCAGCCGGGAGCGGGCGATTGTGATCGTCGCGGATCTCCGCTTCGTAGCAAAACCCCACCCGACCAATCGACGGCCAGCGTCGTT > W3110S.gb/36682‑36818 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |