| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,220,824 | C→A | 19.1% | P235T (CCG→ACG) | ebgR → | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,220,824 | 0 | C | A | 19.1% | 133.6 / 13.2 | 47 | P235T (CCG→ACG) | ebgR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base C (12/26); new base A (6/3); total (18/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.76e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
GCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAAC > W3110S.gb/3220757‑3220887 | gcgcGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCg < 1:1511867/70‑1 (MQ=255)gcgcGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCg < 1:1911810/70‑1 (MQ=255) ggcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAgg > 1:3955576/1‑70 (MQ=255) gTTTTTGCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACtg > 1:658337/1‑71 (MQ=255) gTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACtg < 1:3544764/71‑1 (MQ=255) tttttCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATACGAAGGCACtgt < 1:2897805/71‑1 (MQ=255) tttCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATACGAAGGCACtgtt < 1:1045190/70‑1 (MQ=255) ttCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACtgttt < 1:1048439/70‑1 (MQ=255) ttCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACtgttt < 1:2284688/70‑1 (MQ=255) ttCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACtgttt < 1:2185391/70‑1 (MQ=255) gtcgGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTcc < 1:3645310/70‑1 (MQ=255) gtcgGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTcc < 1:1338099/70‑1 (MQ=255) tcgGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCtt < 1:4692655/67‑1 (MQ=255) tcgGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCtt < 1:2273355/67‑1 (MQ=255) tcgGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATACGAAGGCACtgtttg > 1:579065/1‑61 (MQ=255) ttATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCg < 1:478083/43‑1 (MQ=255) aTGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGaa > 1:4791758/1‑43 (MQ=255) aTGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGaa > 1:4923219/1‑43 (MQ=255) gAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAgg < 1:2151897/43‑1 (MQ=255) gAACTGGCAAAACAAATGCTGGCGCAGGAAGACTATCCGAAgg < 1:3784070/43‑1 (MQ=255) aaCTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCt < 1:3575754/71‑1 (MQ=255) aCTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCa > 1:4627978/1‑43 (MQ=255) aCTGGCAAAACAAATGCTGGCGCGGGAAGACTATACGAAGGCACt > 1:1969100/1‑45 (MQ=255) ggCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCg > 1:120608/1‑71 (MQ=255) ggCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCg > 1:4216451/1‑71 (MQ=255) ggCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCg > 1:3819176/1‑71 (MQ=255) ggCAAAACAAATGCTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCg > 1:4550013/1‑71 (MQ=255) gCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCgg > 1:616196/1‑71 (MQ=255) aaaaCAAATGCTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCg > 1:12337/1‑71 (MQ=255) aaaaCAAATGCTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCg > 1:220969/1‑71 (MQ=255) aaaaCAAATGCTCGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCg < 1:343382/71‑1 (MQ=255) aaaCAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGt < 1:3169307/71‑1 (MQ=255) aaCAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTa > 1:26253/1‑71 (MQ=255) aCAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTAc > 1:3697010/1‑71 (MQ=255) aCAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTAc > 1:779243/1‑71 (MQ=255) cAAATGCTGGCGCGGGAAGACTATACGAAGGCACTGTTTGTTGCTTCCGATTCCa > 1:220676/1‑55 (MQ=255) cTGGCGCTGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGc < 1:785976/71‑1 (MQ=255) gcgcGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAAt < 1:2032743/71‑1 (MQ=255) cgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCa < 1:5105240/71‑1 (MQ=255) cgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCa < 1:4077121/71‑1 (MQ=255) cgGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCa < 1:650218/71‑1 (MQ=255) gggAAGACTATACGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCAt < 1:1835977/71‑1 (MQ=255) aGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAAc < 1:4441759/71‑1 (MQ=255) tCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGc < 1:535179/36‑1 (MQ=255) tCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATg < 1:4481582/62‑1 (MQ=255) tCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATg < 1:4795249/62‑1 (MQ=255) tCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGAGTACTGCGGGCAATTCATg < 1:2400842/62‑1 (MQ=255) | GCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAAC > W3110S.gb/3220757‑3220887 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |