| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,224,816 | C→T | 31.2% | H147Y (CAT→TAT) | ebgC → | cryptic beta‑D‑galactosidase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,224,816 | 0 | C | T | 31.2% | 75.2 / 37.8 | 52 | H147Y (CAT→TAT) | ebgC | cryptic beta‑D‑galactosidase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base C (24/11); new base T (8/8); total (33/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGG > W3110S.gb/3224749‑3224880 | tCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTat < 1:503805/70‑1 (MQ=255) ttATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaa > 1:3864767/1‑71 (MQ=255) ttATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaa > 1:4370253/1‑71 (MQ=255) ttATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaa > 1:356019/1‑71 (MQ=255) cAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATaacaac < 1:1291798/71‑1 (MQ=255) aTAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTa > 1:2630586/1‑71 (MQ=255) aaCGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTAcg > 1:4689746/1‑71 (MQ=255) aCGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTataacaaataa > 1:4609022/1‑62 (MQ=255) aCGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg > 1:3200796/1‑71 (MQ=255) aCGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTAcgg > 1:46428/1‑71 (MQ=255) gTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCa < 1:1891800/71‑1 (MQ=255) aaaaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCataacaaataa > 1:4894565/1‑54 (MQ=255) aaaaaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAg > 1:4214105/1‑71 (MQ=255) aaGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTa > 1:774016/1‑56 (MQ=255) gTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGtt < 1:5130091/71‑1 (MQ=255) ggTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGttt < 1:2623580/70‑1 (MQ=255) tctcAAAGTCACCATCGAAGATGGTTATTTCTATAACAAATAACAACTACGGCGGCAAAAGGAg < 1:2270638/64‑1 (MQ=255) tctcAAAGTCACCATCGAAGATGGTTATTTCCataacaaataacaa > 1:1642020/1‑46 (MQ=255) tctcAAAGTCACCATCGAAGATGGTTATTTCCataacaaataac > 1:2635458/1‑44 (MQ=255) tctcAAAGTCACCATCGAAGATGGTTATTTCCataacaaataac > 1:3861205/1‑44 (MQ=255) ctcAAAGTCACCATCGAAGATGGTTATTTCTataacaaataac < 1:3032025/43‑1 (MQ=255) ctcAAAGTCACCATCGAAGATGGTTATTTCTataacaaataac < 1:1538712/43‑1 (MQ=255) ctcAAAGTCACCATCGAAGATGGTTATTTCCataacaaataac < 1:4076800/43‑1 (MQ=255) ctcAAAGTCACCATCGAAGATGGTTATTTCCataacaaataac < 1:1750649/43‑1 (MQ=255) cAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCa > 1:4661731/1‑71 (MQ=255) cAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCa > 1:1888306/1‑71 (MQ=255) aaaGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgccgcc < 1:3014014/69‑1 (MQ=255) tCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGc > 1:549385/1‑70 (MQ=255) aTCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTAccc > 1:1271553/1‑70 (MQ=255) tCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCaa < 1:1899189/44‑1 (MQ=255) tCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTa > 1:1174104/1‑71 (MQ=255) gAAGATGGTTATTTCTATAACAAATAACAACTACGGCGGCAAAAGGAGTTTg > 1:941365/1‑52 (MQ=255) gAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgcc > 1:4495287/1‑54 (MQ=255) gAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgc > 1:2958825/1‑53 (MQ=255) gAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTa > 1:3808948/1‑64 (MQ=255) gAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTAc > 1:706306/1‑70 (MQ=255) gAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACt > 1:3178330/1‑71 (MQ=255) gAAGATGGTTATTTCAATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgccgc > 1:3789666/1‑56 (MQ=255) tGGTTATTTCTATAACAAATAACAACTACGGCGGCAAAAGGAGttt < 1:1205483/46‑1 (MQ=255) gTTATGTCCATAACAAATAACAACTACGGCGGCaaaa > 1:2290676/1‑37 (MQ=255) ttATTTCTATAACAAATAACAACTACGGCGGCAAAAGGAGTTTg < 1:899925/44‑1 (MQ=255) ttATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCg < 1:3247604/71‑1 (MQ=255) tATTTCTATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCAc < 1:1115049/50‑1 (MQ=255) aTTTCTATAACAAATAACAACTACGGCGGCAAAAGGAGTTTgcc > 1:3223459/1‑44 (MQ=255) aTTTCTATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGc > 1:2276814/1‑52 (MQ=255) aTTTCTATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTc > 1:4893398/1‑68 (MQ=255) aTTTCTATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCg > 1:596489/1‑69 (MQ=255) aTTTCCATAACAAATAACAACTACGGCGGCAAAAGGAg < 1:4299916/38‑1 (MQ=255) tttCTATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCgg < 1:3121777/69‑1 (MQ=255) tttCCATAACAAATAACAACTACGGCGGCAAAAGGa < 1:4572152/36‑1 (MQ=255) ttCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCg > 1:391645/1‑67 (MQ=255) ttCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCg > 1:3962795/1‑67 (MQ=255) ccATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTAcc > 1:4479290/1‑52 (MQ=255) | TCGGTTTATCTGCAATAACGCGGTCAAAAAAGTGGTTCTCAAAGTCACCATCGAAGATGGTTATTTCCATAACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGG > W3110S.gb/3224749‑3224880 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |