Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 3,253,271 | C→T | 43.3% | V99V (GTC→GTT) | yhaK → | predicted pirin‑related protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 3,253,271 | 0 | C | T | 43.3% | 28.5 / 74.9 | 60 | V99V (GTC→GTT) | yhaK | predicted pirin‑related protein |
Reads supporting (aligned to +/- strand): ref base C (11/23); new base T (13/13); total (24/36) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.93e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01 |
GATAGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTG > W3110S.gb/3253203‑3253339 | gATAGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAg < 1:3152185/71‑1 (MQ=255) aTAGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTATACCCAGCCGGGTGTCAGc > 1:3465324/1‑71 (MQ=255) tAGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCt < 1:3675461/71‑1 (MQ=255) aGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTTAGCta > 1:3895582/1‑71 (MQ=255) aGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCta > 1:4449110/1‑71 (MQ=255) gCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTTAGCtat < 1:4101146/71‑1 (MQ=255) gCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTTAGCTATAGCGaa < 1:4024458/71‑1 (MQ=255) aaTCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTTAGCTATAGCGAac < 1:629582/70‑1 (MQ=255) aTCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCTGGTGTTAGCTATAGCGAacac < 1:4442714/71‑1 (MQ=255) aTCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTTAGCTATAGCGAacac < 1:3143676/71‑1 (MQ=255) aTCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTTAGCTATAGCGAacac < 1:2872744/71‑1 (MQ=255) tCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTTAGCTATAGCGAacac > 1:631920/1‑70 (MQ=255) cATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTTAGCTATAGCGAAcaca > 1:3095294/1‑70 (MQ=255) cATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTTAGCTATAGCGAACACaa > 1:3616134/1‑71 (MQ=255) ttCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTTAGCTATAGCGAAcaca < 1:4907763/66‑1 (MQ=255) tCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGc > 1:393834/1‑52 (MQ=255) aGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGc < 1:4149307/71‑1 (MQ=255) ggCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCa < 1:2934563/71‑1 (MQ=255) gCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCaa < 1:3639901/71‑1 (MQ=255) gcCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACa > 1:906049/1‑70 (MQ=255) cGGAGAGGCGTTGCTGCTCTCTACCCTGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCGAAGACAAAc < 1:1507273/71‑1 (MQ=255) ggAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCaaa < 1:2930704/63‑1 (MQ=255) agagGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGa > 1:1399645/1‑63 (MQ=255) gagGCGTTGCTGCTCTCTACCCAGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGt > 1:3762232/1‑70 (MQ=255) gTTGCTGCTCTCTACCCAGCCGGGTGTTAGCTATAGCGaa > 1:3760504/1‑40 (MQ=255) gTTGCTGCTCTCTACCCAGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAAcgc > 1:3099214/1‑71 (MQ=255) gTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACaaa > 1:1007497/1‑61 (MQ=255) ttGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAAcaca < 1:4555067/43‑1 (MQ=255) ttGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAAcgcg < 1:4714300/71‑1 (MQ=255) ttGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAAcgcg < 1:1561291/71‑1 (MQ=255) ttGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAAcgcg < 1:1174306/71‑1 (MQ=255) gctgctCTCTACCCAGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGaa > 1:1160802/1‑71 (MQ=255) gctgctCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGa < 1:5115308/70‑1 (MQ=255) ctgctCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAAt > 1:2664929/1‑71 (MQ=255) ctctctACCCAGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCAAAGACAAAc > 1:3620793/1‑55 (MQ=255) ctctctACCCAGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCAAAGACAAAc > 1:822019/1‑55 (MQ=255) ctctctACCCAGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCAAAGACAAAc > 1:4939484/1‑55 (MQ=255) ctctctACCCAGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGc < 1:90831/70‑1 (MQ=255) ctctctACCCAGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGc < 1:1992865/70‑1 (MQ=255) ctctctACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCa < 1:216652/46‑1 (MQ=255) ctctctACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGc < 1:4598734/70‑1 (MQ=255) ctctctACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGc < 1:4375532/70‑1 (MQ=255) tctACCCAGCCGGGTGTTAGCTATAGAGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAg < 1:1684063/69‑1 (MQ=255) tctACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAAcgcg < 1:669849/62‑1 (MQ=255) ctACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAAcgc > 1:2360765/1‑60 (MQ=255) ctACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAAcgc > 1:3249859/1‑60 (MQ=255) ctACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCtt > 1:3622919/1‑71 (MQ=255) aCCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAAcgcg < 1:4271941/59‑1 (MQ=255) cccAGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCttt < 1:3147467/69‑1 (MQ=255) cccAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCttt < 1:2986910/69‑1 (MQ=255) cccAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTg < 1:2441251/70‑1 (MQ=255) cccAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTg < 1:4365577/70‑1 (MQ=255) gggTGTTAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACg > 1:4691117/1‑71 (MQ=255) gggTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACg < 1:1786641/71‑1 (MQ=255) tgtTAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCt > 1:1778225/1‑71 (MQ=255) gtCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGtt < 1:3654156/41‑1 (MQ=255) gtCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGtt < 1:68356/41‑1 (MQ=255) gtCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTg > 1:278911/1‑71 (MQ=255) tCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCg < 1:768456/38‑1 (MQ=255) tCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTa < 1:3447647/41‑1 (MQ=255) | GATAGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTG > W3110S.gb/3253203‑3253339 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |