| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,259,237 | G→A | 32.0% | S613F (TCT→TTT) | tdcE ← | pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,259,237 | 0 | G | A | 32.0% | 63.8 / 42.3 | 50 | S613F (TCT→TTT) | tdcE | pyruvate formate‑lyase 4/2‑ketobutyrate formate‑lyase |
| Reads supporting (aligned to +/- strand): ref base G (22/12); new base A (6/10); total (28/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
CGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAATTTTCTTCAT > W3110S.gb/3259167‑3259307 | cGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAg < 1:794719/71‑1 (MQ=255) gcgAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGaa > 1:4813629/1‑69 (MQ=255) gcgAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGt < 1:4757252/71‑1 (MQ=255) gcgAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGt < 1:319439/71‑1 (MQ=255) aaCGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGa > 1:1215537/1‑70 (MQ=255) aCGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGa > 1:3002819/1‑69 (MQ=255) gcgcGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGa < 1:5091881/71‑1 (MQ=255) gacgacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTgg < 1:690173/71‑1 (MQ=255) gacgacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTgg > 1:2673970/1‑71 (MQ=255) actacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTggg > 1:4117847/4‑71 (MQ=255) acgacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTggg > 1:1690367/1‑71 (MQ=255) cgacCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCGACTggg > 1:1680746/1‑70 (MQ=255) acCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGt < 1:11623/48‑1 (MQ=255) ccGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCGAc > 1:3258606/1‑63 (MQ=255) cGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGa > 1:4175449/1‑48 (MQ=255) ccGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACg > 1:4264632/1‑71 (MQ=255) ccGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACg > 1:765459/1‑71 (MQ=255) cGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACg > 1:341451/1‑70 (MQ=255) gCGTATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCGACTggg < 1:1870990/60‑1 (MQ=255) tATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATc < 1:1212392/50‑1 (MQ=255) tATTACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCGACTg < 1:248087/55‑1 (MQ=255) ttACCGGTTTTCTGGCCGTACACCACGTTAGAAGt < 1:2235857/35‑1 (MQ=255) ttACCGGTTTTCTGGCCGTACACCACGTTAGAAGt < 1:4076890/35‑1 (MQ=255) ttACCGGTTTTCTGGCCGTACACCACGTTAGAAGt < 1:1157360/35‑1 (MQ=255) ttACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGc < 1:2685978/70‑1 (MQ=255) ttACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGc < 1:1505049/70‑1 (MQ=255) tACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTTCGa > 1:4591172/1‑71 (MQ=255) tACCGGTTTTCTGGCCGTACACCACGTTAAAAGTg > 1:2748792/1‑35 (MQ=255) tACCGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCGa > 1:2500865/1‑49 (MQ=255) aCCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGaa > 1:2993225/1‑44 (MQ=255) aCCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGaa > 1:57217/1‑44 (MQ=255) aCCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGa > 1:2442538/1‑48 (MQ=255) aCCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGa > 1:2139440/1‑48 (MQ=255) aCCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGa > 1:2162741/1‑48 (MQ=255) aCCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGa > 1:1282828/1‑70 (MQ=255) cGGTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCg > 1:1595207/1‑62 (MQ=255) ggTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAgg > 1:3267638/1‑71 (MQ=255) ggTTTTCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAgg > 1:582918/1‑71 (MQ=255) gTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAg > 1:4949372/1‑38 (MQ=255) tttCTGGCCGTACACCACGTTAAAAGTGATAGTCAGAATCGACTGGGTAGGGACGGc < 1:4645722/57‑1 (MQ=255) gCCGTACACCACGTTAAAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAgcg < 1:2256720/71‑1 (MQ=255) gTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTgg < 1:3889919/63‑1 (MQ=255) caccacGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTa < 1:2185586/70‑1 (MQ=255) caccacGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTa < 1:1754471/70‑1 (MQ=255) caccacGTTAAAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAgcg < 1:551085/65‑1 (MQ=255) ccacGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAAtt < 1:6323/71‑1 (MQ=255) cacGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATa > 1:848303/1‑51 (MQ=255) cacGTTAAAAGTGATAGTCAGAATCGACTGGGTAgg < 1:3503242/36‑1 (MQ=255) cGTTAAAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTa < 1:286877/65‑1 (MQ=255) aGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAATTTTCTTCa > 1:4836105/1‑71 (MQ=255) aGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAATTTTCTTCa > 1:2726629/1‑71 (MQ=255) gAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAATTTTCTTCAt < 1:676626/71‑1 (MQ=255) gAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAATTTTCTTCAt < 1:3182343/71‑1 (MQ=255) | CGGCGCGAACGGTGTTCCGGCGCGACGACCGTCCGGCGTATTACCGGTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAATTTTCTTCAT > W3110S.gb/3259167‑3259307 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |