| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,263,996 | C→T | 43.3% | V293M (GTG→ATG) | tdcC ← | L‑threonine/L‑serine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,263,996 | 0 | C | T | 43.3% | 28.6 / 75.0 | 60 | V293M (GTG→ATG) | tdcC | L‑threonine/L‑serine transporter |
| Reads supporting (aligned to +/- strand): ref base C (19/15); new base T (8/18); total (27/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.92e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTAAACAGGCAGCTAAAGGCAAAG > W3110S.gb/3263929‑3264066 | aGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCATTgg < 1:802606/71‑1 (MQ=255)aGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCATTgg < 1:1615923/71‑1 (MQ=255) tgtAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCATTGGaa > 1:297337/1‑71 (MQ=255) tgtAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGaa < 1:909372/71‑1 (MQ=255) gtAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCATTGGaa > 1:2355335/1‑70 (MQ=255) gtAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGaa > 1:352231/1‑70 (MQ=255) tAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCATTGGAata > 1:4136683/1‑71 (MQ=255) tAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAata > 1:1075690/1‑71 (MQ=255) gcgAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAat > 1:2456915/1‑65 (MQ=255) aCGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCt > 1:2346806/1‑71 (MQ=255) gttgttTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCATTGGAatat < 1:217371/61‑1 (MQ=255) tgttTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTgg > 1:2464443/1‑71 (MQ=255) gttTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGc > 1:4968789/1‑71 (MQ=255) gttTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGc > 1:1449688/1‑71 (MQ=255) ttttGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCATTGGAATATTCTGCGCtt < 1:2865799/66‑1 (MQ=255) gTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGc < 1:2947384/71‑1 (MQ=255) tACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCATTGGAATATTCTGCGCTTTGGCTTCCGcc < 1:673408/71‑1 (MQ=255) ccGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCATTGGAATATTCTGCGCTTTGGCTTCCGCCAt < 1:4508279/71‑1 (MQ=255) ccGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCAt < 1:677627/71‑1 (MQ=255) gTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCATTGGAATATTCTGCGCt < 1:2472262/54‑1 (MQ=255) gTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCATTGGAATATTCTGCGCTTTGGCTTCCGCCATGtt < 1:3352467/71‑1 (MQ=255) gTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCATTGGAATATTCTGCGCTTTGGCTTCCGCCATGtt < 1:342960/71‑1 (MQ=255) tCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCATTGGAATATTCTGCGCTTTGGCTTCCGCCATGt > 1:4824337/1‑69 (MQ=255) tCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCATTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTg < 1:2535169/71‑1 (MQ=255) cATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCATTGGAATATTCTGCGCtt > 1:4091394/1‑53 (MQ=255) tGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTgcg < 1:4583880/48‑1 (MQ=255) tGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTgcg < 1:3166717/48‑1 (MQ=255) tGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCttt < 1:4377731/52‑1 (MQ=255) ggACGCAAAGTGGTTAGCCAGATAAGAAAGCATTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCg > 1:4273361/1‑71 (MQ=255) ggACGCAAAGTGGTTAGCCAGATAAGAAAGCATTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCg > 1:335988/1‑71 (MQ=255) gACGCAAAGTGGTTAGCCAGATAAGAAAGCATTGGAATATTCTGCGCTTTGGCTTCCGCCAt < 1:4608344/62‑1 (MQ=255) gACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCt > 1:2986345/1‑54 (MQ=255) gTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACagag > 1:2875612/1‑71 (MQ=255) tGGTTAGCCAGATAAGAAAGCATTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGt < 1:2180680/71‑1 (MQ=255) tGGTTAGCCAGATAAGAAAGCATTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGt < 1:4336706/71‑1 (MQ=255) gTTAGCCAGATAAGAAAGCACTGGAATATTCTgcgc < 1:505508/36‑1 (MQ=255) ttAGCCAGATAAGAAAGCATTGGAATATTCTGCGCTTTGGCTTCCGc > 1:2500857/1‑47 (MQ=255) gCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTAAACa < 1:2892251/70‑1 (MQ=255) cAGATAAGAAAGCATTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACa < 1:3415439/59‑1 (MQ=255) cAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTAAACAgg > 1:349536/1‑70 (MQ=255) aTAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGgag < 1:5066992/53‑1 (MQ=255) aTAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTaa > 1:2671990/1‑62 (MQ=255) aTAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTaa > 1:742974/1‑62 (MQ=255) tAAGAAAGCATTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTAAACAGGCAGCt < 1:297713/71‑1 (MQ=255) tAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTAAACAGGCAGCt < 1:2726959/71‑1 (MQ=255) tAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTAAACAGGCAGCt < 1:561064/71‑1 (MQ=255) aaGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGga < 1:715971/50‑1 (MQ=255) aaGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTaaa > 1:446587/1‑61 (MQ=255) aaGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTaaa > 1:86157/1‑61 (MQ=255) aGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTAAACAGGCAGCTa < 1:3913673/70‑1 (MQ=255) gAAAGCATTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTAAACAGGCAGCTaa < 1:2785519/70‑1 (MQ=255) gAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTAAACAGGCAGCTaaa < 1:1226968/71‑1 (MQ=255) aGCATTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTAAACAGGCAGCTAAAGGc < 1:4307244/71‑1 (MQ=255) aGCATTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTAAACAGGCAGCTAAAGGc < 1:2413839/71‑1 (MQ=255) aGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTAAACAGGCAGCTAAAgg > 1:1740192/1‑70 (MQ=255) gCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGtt > 1:4600859/1‑37 (MQ=255) gCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGtt > 1:4258420/1‑37 (MQ=255) gCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGtt > 1:2745148/1‑37 (MQ=255) gCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGtt > 1:1371962/1‑37 (MQ=255) gCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTAAACAGGCAGCTAAAGGCa < 1:2748151/71‑1 (MQ=255) cTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTAAACAGGCAGCTAAAGGCAAAg < 1:699646/71‑1 (MQ=255) | AGTGTAATCGCGAACGTTGTTTTGGTACCGGTCATGGACGCAAAGTGGTTAGCCAGATAAGAAAGCACTGGAATATTCTGCGCTTTGGCTTCCGCCATGTTGGCCGGAGACAGAGTAAACAGGCAGCTAAAGGCAAAG > W3110S.gb/3263929‑3264066 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |