| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,294,438 | T→C | 38.6% | L395S (TTG→TCG) | yraM → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,294,438 | 0 | T | C | 38.6% | 26.9 / 49.0 | 44 | L395S (TTG→TCG) | yraM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (15/12); new base C (7/10); total (22/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
TTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAAT > W3110S.gb/3294368‑3294499 | ttAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGtt > 1:3258321/1‑71 (MQ=255) cAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTg > 1:2124068/1‑69 (MQ=255) aGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTg > 1:2223577/1‑68 (MQ=255) ggTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGaa > 1:864634/1‑69 (MQ=255) tCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGc > 1:900557/1‑70 (MQ=255) agcagGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTg < 1:832109/62‑1 (MQ=255) gcagGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCaa < 1:3824781/69‑1 (MQ=255) aTGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGaa > 1:2659820/1‑58 (MQ=255) aTGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGaa > 1:3169260/1‑58 (MQ=255) gcgcGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCt > 1:1951248/1‑70 (MQ=255) gAGTATTGTGGTCGGTCCGTTGCTGAAGAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACg > 1:4304512/1‑71 (MQ=255) gAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGc < 1:2973677/55‑1 (MQ=255) aGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGc < 1:1254888/54‑1 (MQ=255) gTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTa > 1:4524909/1‑71 (MQ=255) tATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCaa > 1:1855133/1‑54 (MQ=255) ttGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCaa > 1:3296714/1‑52 (MQ=255) ttGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTg > 1:2660539/1‑71 (MQ=255) ttGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTg > 1:4185070/1‑71 (MQ=255) ttGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTg > 1:248797/1‑71 (MQ=255) tgtgGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGc > 1:3015518/1‑49 (MQ=255) tgtgGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGt < 1:1950125/66‑1 (MQ=255) tgtgGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGt < 1:1162707/66‑1 (MQ=255) gtgGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACg < 1:2164713/64‑1 (MQ=255) gtgGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACg < 1:1985731/64‑1 (MQ=255) gtgGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACg < 1:5034734/64‑1 (MQ=255) gtgGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg < 1:137500/70‑1 (MQ=255) gtgGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg < 1:428341/70‑1 (MQ=255) gtgGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTgg < 1:4180912/70‑1 (MQ=255) tgGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTc > 1:3854255/1‑54 (MQ=255) tgGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCa > 1:1334837/1‑71 (MQ=255) gtcggtcCGTTGCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACt > 1:1104613/1‑51 (MQ=255) gTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGt < 1:315751/54‑1 (MQ=255) gTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGt < 1:171338/54‑1 (MQ=255) gTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGt < 1:1505848/54‑1 (MQ=255) gTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGt < 1:833824/54‑1 (MQ=255) gCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCg > 1:2531871/1‑70 (MQ=255) gCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCg < 1:4660769/70‑1 (MQ=255) gCTGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAAcc > 1:3639278/1‑65 (MQ=255) tGAAAAATAACGTTGAAGAGTCGCTGAAGAGCAACACTCCGCTg < 1:4275863/44‑1 (MQ=255) aaaaaTAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGgag < 1:4483456/69‑1 (MQ=255) aaaaaTAACGTTGAAGAGTCTCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGgaga < 1:973355/70‑1 (MQ=255) aaaaTAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGgaga > 1:4616745/1‑69 (MQ=255) aaaaTAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGaa > 1:926759/1‑70 (MQ=255) aaaTAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAata < 1:3475215/71‑1 (MQ=255) ttGAAGAGTCGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAAt < 1:1106274/71‑1 (MQ=255) | TTAAGCCAGGTTCAGCAGGATGGCGCGAGTATTGTGGTCGGTCCGTTGCTGAAAAATAACGTTGAAGAGTTGCTGAAGAGCAACACTCCGCTGAACGTACTGGCACTGAACCAGCCGGAGAATATCGAAAAT > W3110S.gb/3294368‑3294499 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |