| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,306,434 | G→A | 33.3% | L428L (CTG→TTG) | deaD ← | ATP‑dependent RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,306,434 | 0 | G | A | 33.3% | 73.9 / 53.9 | 60 | L428L (CTG→TTG) | deaD | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base G (25/15); new base A (6/14); total (31/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.78e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.49e-01 | |||||||||||
CGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTCAG > W3110S.gb/3306376‑3306497 | cGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCag < 1:4970383/71‑1 (MQ=255) cATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCagag > 1:1063968/1‑71 (MQ=255) tCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGtt < 1:2802052/71‑1 (MQ=255) tCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGtt < 1:1149536/71‑1 (MQ=255) tCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGtt < 1:1997272/71‑1 (MQ=255) tCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGtt > 1:400794/1‑71 (MQ=255) ggCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTc > 1:4018349/1‑71 (MQ=255) gcgcATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCg < 1:1375778/71‑1 (MQ=255) gcATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTc > 1:1708629/1‑68 (MQ=255) gcATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCgag < 1:3149509/71‑1 (MQ=255) gcATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCgag < 1:2240027/71‑1 (MQ=255) cATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCgag < 1:3406650/70‑1 (MQ=255) cTGGCGATACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATc > 1:654989/1‑70 (MQ=255) tGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATc > 1:1390785/1‑69 (MQ=255) tGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATc > 1:1107762/1‑69 (MQ=255) ggCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGt < 1:3106783/59‑1 (MQ=255) gCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTTCCGCAGCCAGAGTTTCGAGATcc < 1:345999/68‑1 (MQ=255) gCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATcc < 1:618142/68‑1 (MQ=255) cGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGct < 1:497519/71‑1 (MQ=255) ggTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCagag > 1:2394386/1‑55 (MQ=255) gTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCaga < 1:3312310/53‑1 (MQ=255) aCGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGttt > 1:5001575/1‑55 (MQ=255) aCGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGc > 1:4016753/1‑66 (MQ=255) aCGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCtt > 1:3854104/1‑70 (MQ=255) cGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCa < 1:4715112/71‑1 (MQ=255) cGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTc < 1:2166239/70‑1 (MQ=255) aTCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCag < 1:804022/47‑1 (MQ=255) aTCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTc > 1:1276662/1‑53 (MQ=255) aTCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTc > 1:3522010/1‑53 (MQ=255) aTCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTc > 1:2499522/1‑53 (MQ=255) aTCAGAGTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGctc < 1:2918480/65‑1 (MQ=255) agagTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCga > 1:2948862/1‑52 (MQ=255) agagTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCga > 1:2999975/1‑52 (MQ=255) agagTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCga > 1:132769/1‑52 (MQ=255) agagTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTc < 1:3945434/71‑1 (MQ=255) gagTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTc < 1:29285/49‑1 (MQ=255) agTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTcag < 1:3336382/71‑1 (MQ=255) agTACGTTCACCCTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTcag < 1:2421997/71‑1 (MQ=255) cGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGtt > 1:4388209/1‑42 (MQ=255) cGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGt > 1:2033128/1‑71 (MQ=255) cGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGt > 1:2977118/1‑71 (MQ=255) cGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGt > 1:1089109/1‑71 (MQ=255) cGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGt > 1:3904240/1‑71 (MQ=255) ttCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCAc > 1:3266925/1‑59 (MQ=255) ttCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCAc > 1:1680601/1‑59 (MQ=255) cTGTGCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGc < 1:3742742/46‑1 (MQ=255) tgtgCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATcc > 1:1049658/1‑42 (MQ=255) tgtgCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGct > 1:4309721/1‑46 (MQ=255) gtgCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAtt > 1:3745464/1‑71 (MQ=255) tgCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAt < 1:1600240/69‑1 (MQ=255) tgCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAt < 1:2782888/69‑1 (MQ=255) tgCCATTTTCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAAt < 1:2858022/69‑1 (MQ=255) ttttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGc < 1:4927286/38‑1 (MQ=255) ttttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCt > 1:2815401/1‑70 (MQ=255) tttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTc > 1:3361277/1‑70 (MQ=255) ttCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTcag > 1:4612341/1‑71 (MQ=255) ttCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTcag > 1:1644477/1‑71 (MQ=255) tCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTcag < 1:4608708/70‑1 (MQ=255) tCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTcag < 1:1730300/70‑1 (MQ=255) tCAGCAATGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTcag < 1:408411/70‑1 (MQ=255) gcaaTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTg < 1:1966595/55‑1 (MQ=255) | CGCATCGGCGCATCTGGCGGTACGATCAGAGTACGTTCACCCTGTGCCATTTTCAGCAGTGCCGCAGCCAGAGTTTCGAGATCCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTCAG > W3110S.gb/3306376‑3306497 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |