| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 241,172 | C→T | 31.6% | G711D (GGT→GAT) | fadE ← | acyl coenzyme A dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 241,172 | 0 | C | T | 31.6% | 79.4 / 47.4 | 57 | G711D (GGT→GAT) | fadE | acyl coenzyme A dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (21/18); new base T (11/7); total (32/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAATCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGT > W3110S.gb/241104‑241241 | cAATCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACcg > 1:1472487/1‑71 (MQ=255) aaTCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACcgc > 1:2994441/1‑71 (MQ=255) aTCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGATcgc > 1:2945990/1‑70 (MQ=255) aTCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACcgcg < 1:2867964/71‑1 (MQ=255) aTCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACcgc > 1:3463826/1‑70 (MQ=255) aTCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACcgc > 1:2750334/1‑70 (MQ=255) cacaTCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGATCGCGAc > 1:4793714/1‑71 (MQ=255) cacaTCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACcgcg > 1:4583642/1‑69 (MQ=255) cacaTCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGAc > 1:372508/1‑71 (MQ=255) acaTCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGAc > 1:4444598/1‑70 (MQ=255) caccaGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCACGGCGTCAGGTACTGACCGCGACCAATg < 1:3022323/70‑1 (MQ=255) cgcCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGATCGCGACCAATGCGGGAAc < 1:2654518/71‑1 (MQ=255) gcCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGATcgc < 1:1821993/55‑1 (MQ=255) gcCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGATcgc < 1:3206084/55‑1 (MQ=255) gcCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGATcgc < 1:276215/55‑1 (MQ=255) tctTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGATCGCGAcc < 1:1505643/56‑1 (MQ=255) ctTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAAc < 1:1082760/66‑1 (MQ=255) ttCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCaa > 1:3753585/1‑56 (MQ=255) ccAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCg > 1:2398747/1‑71 (MQ=255) cAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGAcc < 1:1027284/51‑1 (MQ=255) aGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGAcc < 1:3678055/50‑1 (MQ=255) gcaagcaaACCGGATTATGCTCGCTCGGCGTCAGGTACTGATCGCGACCAATGCGGGAACGGGTGGCGTTc < 1:2341622/71‑1 (MQ=255) gCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGATCGCGACCAATGCGGGAACGGGTGGCGTTc > 1:1771412/1‑71 (MQ=255) cAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGAGACCAATGCGGGaa > 1:2340621/1‑53 (MQ=255) aCCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTg < 1:4200344/71‑1 (MQ=255) cGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCaa > 1:3815318/1‑41 (MQ=255) cGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCaa > 1:760078/1‑41 (MQ=255) gATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGaa > 1:4233428/1‑47 (MQ=255) gATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTaa > 1:2806779/1‑70 (MQ=255) gATTATGCTCCCTCGGCGTCAGGTACTGACCGCGACCAATGCGTGAACGGGTGGCGTTCGGCACTTGTaa > 1:4037820/1‑70 (MQ=255) tATGCTCGCTCTGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCg < 1:1602530/53‑1 (MQ=255) tATGCTCGCTCGGCGTCAGGTACTGATCGCGACCAATGCGGGAACggg > 1:828848/1‑48 (MQ=255) tATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCg < 1:1322970/53‑1 (MQ=255) aaTGCTCGCTCGGCGTCAGGTACTGATCGCGACCAATGCGGGAACggg > 1:3492603/2‑48 (MQ=39) aTGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATc < 1:4658063/70‑1 (MQ=255) tGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGtt > 1:304978/1‑53 (MQ=255) ctcgctcgGCGTCAGGTACTGATCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTc > 1:1276786/1‑70 (MQ=255) ctcgctcgGCGTCAGGTACTGATCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCg > 1:1030548/1‑71 (MQ=255) ctcgctcgGCGTCAGGTACTGATCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCg > 1:2600614/1‑71 (MQ=255) tcgGCGTCAGGTACTGACCGCGACCAATGCGGGAACgg > 1:4827991/1‑38 (MQ=255) cgGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACtt > 1:3873862/1‑71 (MQ=255) ggCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACttt < 1:1044846/71‑1 (MQ=255) ggCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACttt < 1:2134921/71‑1 (MQ=255) gCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTa > 1:3680864/1‑71 (MQ=255) cGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTc < 1:4269130/43‑1 (MQ=255) tCAGGTACTGATCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGa < 1:2965392/71‑1 (MQ=255) tCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGa < 1:4167155/71‑1 (MQ=255) aGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGAt < 1:3558517/70‑1 (MQ=255) gTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCa < 1:1423429/71‑1 (MQ=255) aCTGATCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGc > 1:3378880/1‑71 (MQ=255) cTGATCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACtt > 1:4268722/1‑59 (MQ=255) cTGATCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACtt > 1:3315596/1‑59 (MQ=255) tGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGc < 1:1691535/69‑1 (MQ=255) tGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGc < 1:735142/69‑1 (MQ=255) gACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTg > 1:2211728/1‑71 (MQ=255) gACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTg > 1:2217531/1‑71 (MQ=255) aCCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTg < 1:3339718/70‑1 (MQ=255) ccGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGt > 1:598699/1‑70 (MQ=255) | CAATCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGT > W3110S.gb/241104‑241241 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |