| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,317,696 | G→A | 7.5% | H56Y (CAC→TAC) | yhbC ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,317,696 | 0 | G | A | 7.5% | 343.4 / 12.6 | 107 | H56Y (CAC→TAC) | yhbC | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (52/47); new base A (4/4); total (56/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01 | |||||||||||
Only 100 of 119 total aligned reads displayed.
GAGGAGACTTCCAGGTTATAAGCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAATATAGATGCGCAGTGTGGATGTG > W3110S.gb/3317629‑3317766 | gaggagACTTCCAGGTTATAAGCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCt > 1:3558383/1‑71 (MQ=255) gaCTTCCAGGTTATAAGCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACAt < 1:535255/71‑1 (MQ=255) cAGGTTATAAGCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGca > 1:862458/1‑70 (MQ=255) aGGTTATAAGCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGcaca < 1:1713454/71‑1 (MQ=255) ggTTATAAGCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGcaca > 1:2847723/1‑70 (MQ=255) gTTATAAGCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAat < 1:1376915/71‑1 (MQ=255) ttATAAGCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAat > 1:2243371/1‑70 (MQ=255) ttATAAGCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTAGCTCACATCAGCACAatc < 1:3109913/71‑1 (MQ=255) ttATAAGCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTAGCTCACATCAGCACAat > 1:4141915/1‑70 (MQ=255) tataAGCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTca > 1:1435319/1‑57 (MQ=255) ataAGCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAatcat > 1:1928424/1‑71 (MQ=255) ataAGCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAatcat > 1:3338118/1‑71 (MQ=255) taAGCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAatcatc < 1:2647133/71‑1 (MQ=255) aGCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCaa > 1:3327443/1‑71 (MQ=255) aGCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCaa < 1:3838311/71‑1 (MQ=255) aGCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCaa < 1:5009833/71‑1 (MQ=255) gCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCt > 1:3778362/1‑50 (MQ=255) gCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAAtcatca < 1:1361030/69‑1 (MQ=255) gCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCaa > 1:1670553/1‑70 (MQ=255) aCGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACAtt < 1:4509584/71‑1 (MQ=255) cGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAatca < 1:2858406/62‑1 (MQ=255) cGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTg < 1:2588190/71‑1 (MQ=255) gTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGa > 1:4413662/1‑70 (MQ=255) gATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTc > 1:600907/1‑43 (MQ=255) aTGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATGAACAtt < 1:657667/65‑1 (MQ=255) aTGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACAtt < 1:3306854/65‑1 (MQ=255) aTGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGc < 1:248496/70‑1 (MQ=255) aTGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGc > 1:1597870/1‑70 (MQ=255) ggATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTca > 1:3716535/1‑40 (MQ=255) ggATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTca > 1:1182354/1‑40 (MQ=255) ggATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATc > 1:1055890/1‑71 (MQ=255) gATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATc < 1:3701208/70‑1 (MQ=255) aTCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCt > 1:2332098/1‑70 (MQ=255) cTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGcc < 1:4148837/64‑1 (MQ=255) cTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCtt > 1:22396/1‑69 (MQ=255) cTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCtt > 1:1197516/1‑69 (MQ=255) cTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCtt > 1:1664171/1‑69 (MQ=255) ttCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGca < 1:2902654/43‑1 (MQ=255) tCAACATCCAGCACAGCACTTACCTGGTGGCTCACAt < 1:1672741/37‑1 (MQ=255) tCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCaa > 1:1601533/1‑52 (MQ=255) tCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCaa > 1:1333264/1‑52 (MQ=255) tCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCaa > 1:3748090/1‑52 (MQ=255) tCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCAc > 1:5075243/1‑70 (MQ=255) gaaCATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCtt < 1:1450376/65‑1 (MQ=255) cAACATCCTGCACAGCACTTACCTGGTGGCTCACATCAGCACAatcatc < 1:277000/49‑1 (MQ=255) cAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGa > 1:3467853/1‑57 (MQ=255) cAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTa > 1:2513687/1‑71 (MQ=255) cAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTa > 1:4982867/1‑71 (MQ=255) cAACATCCAGCACAGCACTTACCTGGTGGCTCAAATCAGCACAATCATCAAc > 1:2184980/1‑52 (MQ=255) cAACATCCAGCACAGCACTTACCTGGTAGCTCACATCAGCACAATCATCAACATTg > 1:2755246/1‑56 (MQ=255) cAACATCCAGCACAGCACTTACCTGGTAGCTCACATCAGCACAATCATCAACATTGATGCCATCtt < 1:171147/66‑1 (MQ=255) cAACATCCAGCACAGCACTTACCTGGTAGCTCACATCAGCACAATCATCAACATTGATGCCATCtt < 1:2330443/66‑1 (MQ=255) cAACATCCAGCACAGCACTTACCTGGTAGCTCACATCAGCACAATCATCAACATTGATGCCATCtt < 1:4339282/66‑1 (MQ=255) cAACATCCAGCAAAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTa > 1:4910804/1‑71 (MQ=255) aaCATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCaa > 1:1871360/1‑50 (MQ=255) aaCATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCaa > 1:2049260/1‑50 (MQ=255) aCATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATc < 1:4035537/71‑1 (MQ=255) cATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACAtt < 1:3963517/52‑1 (MQ=255) cATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATc > 1:90459/1‑70 (MQ=255) ccAGCACAGCACTTACCTGGTGGCTCACATCAGCACAatca > 1:3431835/1‑41 (MQ=255) cagcacagcaCTTACCTGGTGGCTCACATCAGCACAatcatc < 1:1576551/42‑1 (MQ=255) cagcacagcaCTTACCTGGTGGCTCACATCAGCACAatcatc < 1:1246267/42‑1 (MQ=255) cagcacagcaCTTACCTGGTGGCTCACATCAGCACAATCATCAACa > 1:639774/1‑46 (MQ=255) cagcacagcaCTTACCTGGTGGCTCACATCAGCACAATCATCAACa > 1:2493006/1‑46 (MQ=255) cagcacagcaCTTACCTGGTGGCTCACATCAGCACAATCATCAACa > 1:3512824/1‑46 (MQ=255) agcacagcacTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAAtata < 1:3741877/71‑1 (MQ=255) agcacagcacTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAAtata < 1:3216429/71‑1 (MQ=255) gcacagcacTTACGTGGTGGCTCACATCAGCACAatcatc < 1:1171578/40‑1 (MQ=255) gcacagcacTTACCTGGTGGCTCACATCAGCACAatcatc < 1:4279523/40‑1 (MQ=255) gcacagcacTTACCTGGTGGCTCACATCAGCACAatcatc < 1:4095607/40‑1 (MQ=255) gcacagcacTTACCTGGTGGCTCACATCAGCACAATCATCaa > 1:785865/1‑42 (MQ=255) gcacagcacTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCaa < 1:1835794/66‑1 (MQ=255) gcacagcacTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCaa < 1:4077399/66‑1 (MQ=255) cacagcacTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAata > 1:3093812/1‑67 (MQ=255) cacTTATCTGGTGGCTCTCATCAGCACAATCATCAACATTGATGCCATCTTCACTATCaa < 1:4776587/60‑1 (MQ=255) cacTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAATATAGATgcgc < 1:2277724/71‑1 (MQ=255) cacTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAATATAGATgcg > 1:1648054/1‑70 (MQ=255) cTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTa < 1:2092093/54‑1 (MQ=255) cTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTa < 1:2086085/54‑1 (MQ=255) cTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTa < 1:3335509/54‑1 (MQ=255) ttACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAAtata < 1:686784/61‑1 (MQ=255) ttACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAAtata < 1:3178831/61‑1 (MQ=255) tACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCAc > 1:5030965/1‑50 (MQ=255) tACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCAc > 1:211760/1‑50 (MQ=255) aCCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCAt > 1:2475522/1‑43 (MQ=255) aCCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAATATAGATGCGCAgtgt > 1:1926294/1‑71 (MQ=255) aCCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAATATAGATGCGCAgtgt > 1:4685079/1‑71 (MQ=255) aCCTGGTAGCTCACATCAGCACAATCATCAACATTGa > 1:1303917/1‑37 (MQ=255) ccTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAATATAGATGCGCAGtgtg < 1:3735229/71‑1 (MQ=255) ccTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAATATAGATGCGCAGtgtg < 1:3684459/71‑1 (MQ=255) ccTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAATATAGATGCGCAGtgtg < 1:53635/71‑1 (MQ=255) ggtggCTCACATCAGCACAATCATCAACATTGATGCCATCTTCa > 1:1739579/1‑44 (MQ=255) ggtggCTCACATCAGCACAATCATCAACATTGATGCCATCTTCAc > 1:1076731/1‑45 (MQ=255) ggtggCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAata > 1:3113511/1‑53 (MQ=255) ggtggCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAATATAGATgcgc > 1:1341420/1‑62 (MQ=255) gtggCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTAt < 1:643663/47‑1 (MQ=255) gtggCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAATATAGATGCGCAGTGTGGAt < 1:4546681/70‑1 (MQ=255) ggCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCa > 1:472384/1‑47 (MQ=255) ggCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAATATAGATGCGCAGTGTGGAtgtg > 1:4311335/1‑71 (MQ=255) ggCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAATATAGATGCGCAGTGTGGAtgtg > 1:3419738/1‑71 (MQ=255) | GAGGAGACTTCCAGGTTATAAGCAACGGTGATGGGATCTTCAACATCCAGCACAGCACTTACCTGGTGGCTCACATCAGCACAATCATCAACATTGATGCCATCTTCACTATCAATATAGATGCGCAGTGTGGATGTG > W3110S.gb/3317629‑3317766 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |