| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,320,162 | T→C | 5.5% | Y436C (TAT→TGT) | yhbX ← | predicted hydrolase, inner membrane |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,320,162 | 0 | T | C | 5.5% | 212.1 / 3.1 | 73 | Y436C (TAT→TGT) | yhbX | predicted hydrolase, inner membrane |
| Reads supporting (aligned to +/- strand): ref base T (41/28); new base C (2/2); total (43/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
ACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCG > W3110S.gb/3320093‑3320229 | aCGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATa < 1:2753244/71‑1 (MQ=255) tataCCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGttc > 1:1276558/1‑71 (MQ=255) taCCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGttctt < 1:3915013/71‑1 (MQ=255) taCCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAACAGACGttctt > 1:955902/1‑71 (MQ=255) taCCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAACAGACGttct > 1:571866/1‑70 (MQ=255) cAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGttcttctt > 1:3790409/1‑71 (MQ=255) aTAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGt < 1:292854/71‑1 (MQ=255) aTAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGt < 1:707029/71‑1 (MQ=255) aaCATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAg < 1:3866863/54‑1 (MQ=255) aCATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAg < 1:5069272/53‑1 (MQ=255) aCATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTa < 1:2663765/68‑1 (MQ=255) cATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGt > 1:5073348/1‑71 (MQ=255) aTCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAgg > 1:4991760/1‑68 (MQ=255) aTCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGt > 1:1683142/1‑70 (MQ=255) tCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCa < 1:699899/71‑1 (MQ=255) cGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCAc > 1:3042558/1‑71 (MQ=255) gggACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACg > 1:4655900/1‑71 (MQ=255) cATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATACACGTTCTTCTTCGTAGGGTCACGTTcc < 1:3642876/71‑1 (MQ=255) aTGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATCAAAATAGACGTTCTTCTTCGTAGGGTCACGTTc > 1:3442427/1‑69 (MQ=255) gATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGttc > 1:5075936/1‑47 (MQ=255) gATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCAc < 1:666266/63‑1 (MQ=255) gATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAACAGACGtt < 1:923689/46‑1 (MQ=255) atatGCCTTCTGGCTAGCCTCCCTGCCTCCATGAAAACAGACGtt < 1:1806879/45‑1 (MQ=255) atatGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGc > 1:5044481/1‑71 (MQ=255) tGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATa < 1:647665/36‑1 (MQ=255) ccTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGa > 1:572489/1‑36 (MQ=255) ccTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTa > 1:3941065/1‑50 (MQ=255) ccTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTgg > 1:3144901/1‑71 (MQ=255) ccTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTgg > 1:4995752/1‑71 (MQ=255) ccTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTgg > 1:4841760/1‑71 (MQ=255) ccTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTg > 1:4167270/1‑70 (MQ=255) ccTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTg > 1:1248734/1‑70 (MQ=255) ccTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTg > 1:4980478/1‑70 (MQ=255) tgctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTa < 1:3101780/48‑1 (MQ=255) tgctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTa < 1:3219025/48‑1 (MQ=255) gctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGt < 1:841313/36‑1 (MQ=255) ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCgg > 1:4362502/1‑71 (MQ=255) ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCgg > 1:2052587/1‑71 (MQ=255) ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCgg > 1:1451015/1‑71 (MQ=255) gCTAGCCTCCCTGCCTCCATGAAAATAGGCGTTCTTCTTCGTAGGGGCACGTTCCAGGCCGTGGTCGGCaa < 1:589306/71‑1 (MQ=255) tAGCCTCCCTGCCTCCATGAAAATAGACGttcttcttc > 1:4843630/1‑38 (MQ=255) tAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCg > 1:1150452/1‑39 (MQ=255) cccTGCCTCCATGAAAATAGACGTTCTTCTTCGTa < 1:102176/35‑1 (MQ=255) ccTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGt > 1:4314657/1‑56 (MQ=255) tGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGt < 1:332140/41‑1 (MQ=255) tGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGt < 1:2768813/41‑1 (MQ=255) ccTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGtt > 1:2621389/1‑40 (MQ=255) ccTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTc > 1:3538140/1‑41 (MQ=255) cTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGt < 1:1424193/38‑1 (MQ=255) tCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCaaaa > 1:2614507/1‑58 (MQ=255) ccATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAg > 1:1454838/1‑41 (MQ=255) ccATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTgg < 1:3466198/48‑1 (MQ=255) ccATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGACCGAGgc > 1:3470568/1‑71 (MQ=255) cATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTgg < 1:1862263/47‑1 (MQ=255) cATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGACCGAGgcg > 1:1291186/1‑71 (MQ=255) cATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGACCGAGgcg > 1:4954883/1‑71 (MQ=255) aTGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAg > 1:1516235/1‑39 (MQ=255) aTGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAg > 1:1941925/1‑39 (MQ=255) tGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGt < 1:3764636/46‑1 (MQ=255) tGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGt < 1:2849782/46‑1 (MQ=255) tGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACa > 1:3245199/1‑58 (MQ=255) tGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACa > 1:3925063/1‑58 (MQ=255) tGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGcgcg > 1:4948956/1‑71 (MQ=255) gAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGt < 1:3638958/45‑1 (MQ=255) gAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACAt < 1:5048605/58‑1 (MQ=255) gAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACAt < 1:3053552/58‑1 (MQ=255) gAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCgcgg > 1:448349/1‑71 (MQ=255) aaaaTAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCgcggc > 1:2711822/1‑71 (MQ=255) aaaaTAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCgcggc > 1:3107781/1‑71 (MQ=255) aaaTAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGa > 1:2069673/1‑58 (MQ=255) aaaTAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCgcggcg < 1:4520330/71‑1 (MQ=255) aaaTAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCgcggcg < 1:1221912/71‑1 (MQ=255) aaTAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGt > 1:1047622/1‑42 (MQ=255) | ACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCG > W3110S.gb/3320093‑3320229 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |