| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,332,615 | T→A | 36.6% | intergenic (‑25/+102) | yhbE ← / ← rpmA | conserved inner membrane protein/50S ribosomal subunit protein L27 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,332,615 | 0 | T | A | 36.6% | 82.6 / 88.6 | 82 | intergenic (‑25/+102) | yhbE/rpmA | conserved inner membrane protein/50S ribosomal subunit protein L27 |
| Reads supporting (aligned to +/- strand): ref base T (17/35); new base A (13/17); total (30/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGG > W3110S.gb/3332548‑3332685 | tggtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCg < 1:547812/71‑1 (MQ=255)tggtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCg < 1:1317925/71‑1 (MQ=255)tggtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCg < 1:719336/71‑1 (MQ=255)tggtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCg < 1:4574511/71‑1 (MQ=255) gtgAGCGGCAAAAGAATGACAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGa > 1:1406770/1‑71 (MQ=255) gtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGa > 1:3201096/1‑71 (MQ=255) gtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGa > 1:696244/1‑71 (MQ=255) gtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGa < 1:1578816/71‑1 (MQ=255) tgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGAt > 1:2337112/1‑71 (MQ=255) aGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAtt < 1:2807692/70‑1 (MQ=255) cgcCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAAc < 1:2556404/71‑1 (MQ=255) cgcCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGa < 1:182268/66‑1 (MQ=255) cgcCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAAc < 1:809345/71‑1 (MQ=255) cgcCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAAc < 1:3921985/71‑1 (MQ=255) cgcCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAAc < 1:1345360/71‑1 (MQ=255) cAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTc < 1:1087703/71‑1 (MQ=255) aaaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGc < 1:3371212/71‑1 (MQ=255) aaaGAATGCCAATGCCTGCCTGCTACTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCg > 1:229296/1‑70 (MQ=255) aaaGAATGCCAATGCCTGCCTGCGGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCg > 1:3039334/1‑70 (MQ=255) aaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCa > 1:1729581/1‑71 (MQ=255) aaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCa > 1:3497329/1‑71 (MQ=255) gccaatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCAcc < 1:351251/67‑1 (MQ=255) gccaatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCAcc < 1:2294658/67‑1 (MQ=255) ccaatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACg < 1:3535090/59‑1 (MQ=255) caatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATgcgc < 1:1014605/71‑1 (MQ=255) gcctgcctGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCg > 1:4224311/1‑46 (MQ=255) gcctgcctGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCAtt > 1:647421/1‑70 (MQ=255) cctgcctgCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtttt > 1:963354/1‑71 (MQ=255) cctgcctgCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAtttt < 1:4238000/71‑1 (MQ=255) ctgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAttttt < 1:1141163/71‑1 (MQ=255) ctgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAttttt < 1:1135095/71‑1 (MQ=255) ctgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCAttttt < 1:137013/71‑1 (MQ=255) tgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCg < 1:4017764/43‑1 (MQ=255) tgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAtt < 1:1312450/47‑1 (MQ=255) tgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAtt < 1:3816281/47‑1 (MQ=255) tgcctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAtt < 1:4495116/47‑1 (MQ=255) gcctgcTGCTTCATACCGTATTCCCTGCAAAGCGAATTTGCGGATTAACGTCGCACCATGCGCATTTTTAc > 1:116755/1‑71 (MQ=255) cctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTg > 1:1022968/1‑39 (MQ=255) cctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTaa > 1:2172559/1‑47 (MQ=255) cctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTaa > 1:2092266/1‑47 (MQ=255) cctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTaa > 1:3705881/1‑47 (MQ=255) cctgcTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTaa > 1:5136627/1‑47 (MQ=255) tgctgcTTCATACCGTATTCCCTGCAAAGCGCATTAGCGGAtt < 1:3388967/43‑1 (MQ=255) tgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAtt < 1:2972685/40‑1 (MQ=255) tgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAtt < 1:4610836/40‑1 (MQ=255) tgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAtt < 1:779399/40‑1 (MQ=255) tgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAtt < 1:1189832/40‑1 (MQ=255) tgctTCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTAc > 1:3058304/1‑65 (MQ=255) gctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAtt < 1:606488/39‑1 (MQ=255) gctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAtt < 1:722060/39‑1 (MQ=255) gctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAtt < 1:2062846/39‑1 (MQ=255) gctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGAtt < 1:4119142/39‑1 (MQ=255) ctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTa < 1:359777/69‑1 (MQ=255) ctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTa < 1:3869005/69‑1 (MQ=255) ttCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTaa > 1:709661/1‑39 (MQ=255) ttCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTaa > 1:3562444/1‑39 (MQ=255) ttCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTaa > 1:883601/1‑39 (MQ=255) ttCATACCGTATTCCCTGCAAAGCGCATTAGCGGATTaa > 1:3840108/1‑39 (MQ=255) cATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGa < 1:679504/70‑1 (MQ=255) cATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGa < 1:4225350/70‑1 (MQ=255) cATACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGa < 1:4219449/70‑1 (MQ=255) aTACCGTATTCCCTGCGAAGCGCATTAGCGGATTAACGTCGc < 1:3365156/42‑1 (MQ=38) aTACCGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCg < 1:148905/68‑1 (MQ=255) aCCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATg > 1:3867663/1‑70 (MQ=255) ccGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGt < 1:3491888/70‑1 (MQ=255) ccGTATTCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATg > 1:2137003/1‑69 (MQ=255) cGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTa < 1:401993/70‑1 (MQ=255) gTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTaaa < 1:1909424/71‑1 (MQ=255) ttCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGa < 1:3513066/61‑1 (MQ=255) ttCCCTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTaaaaa < 1:771573/70‑1 (MQ=255) ttCCCAGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTaaaaa < 1:3499599/70‑1 (MQ=255) cccTGCAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGc < 1:2054732/70‑1 (MQ=255) ccTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAt > 1:295479/1‑42 (MQ=255) cAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCAttttt < 1:2712205/42‑1 (MQ=255) cAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTAc > 1:3473727/1‑44 (MQ=255) cAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCa < 1:1258104/71‑1 (MQ=255) cAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCa < 1:2632787/71‑1 (MQ=255) cAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGa > 1:2445734/1‑54 (MQ=255) cAAAGCGCATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGa > 1:2541300/1‑54 (MQ=255) gcgcATTAGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGcc > 1:2569296/1‑62 (MQ=255) cgcATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTaaa > 1:3660735/1‑56 (MQ=255) aTTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTg < 1:4350454/71‑1 (MQ=255) tttGCGGATTAACGTCGCACCATGCGCATTTTTACCg < 1:4848718/37‑1 (MQ=255) ttGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTaaaaa < 1:1153742/53‑1 (MQ=255) tGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGc > 1:1943708/1‑54 (MQ=255) tGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGc > 1:845000/1‑54 (MQ=255) tGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCgg > 1:4349115/1‑71 (MQ=255) | TGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGG > W3110S.gb/3332548‑3332685 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |