| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,338,549 | G→A | 39.7% | F185F (TTC→TTT) | yrbE ← | predicted toluene transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,338,549 | 0 | G | A | 39.7% | 48.1 / 71.2 | 63 | F185F (TTC→TTT) | yrbE | predicted toluene transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (15/23); new base A (12/13); total (27/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01 | |||||||||||
CACGCTCTTAATCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGC > W3110S.gb/3338483‑3338614 | cacGCTCTTAATCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCagaaga < 1:2825965/71‑1 (MQ=255)cacGCTCTTAATCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCagaaga < 1:746500/71‑1 (MQ=255) tAATCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTa > 1:4103967/1‑71 (MQ=255) aTCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATc < 1:945020/71‑1 (MQ=255) aTCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATc < 1:3368489/71‑1 (MQ=255) aTCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATc < 1:220440/71‑1 (MQ=255) tCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATc > 1:3703701/1‑70 (MQ=255) gACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAAt > 1:2144966/1‑70 (MQ=255) gTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATg < 1:132552/67‑1 (MQ=255) gTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATg < 1:64762/67‑1 (MQ=255) gTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCtt < 1:849669/71‑1 (MQ=255) tGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTc > 1:2375849/1‑71 (MQ=255) tGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTc > 1:4611001/1‑71 (MQ=255) aCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCagaaga < 1:2135226/49‑1 (MQ=255) aCCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTcc < 1:5105322/70‑1 (MQ=255) cAGATCCATACGCCAGTCGACGGCATTATGCATTGCCGACCAGAAGAACCCGCTATCAATGACTTTCCAAc > 1:2210561/1‑71 (MQ=255) aGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACt < 1:1676664/71‑1 (MQ=255) aGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACt < 1:1237081/71‑1 (MQ=255) aTCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATc < 1:4060064/54‑1 (MQ=255) cGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGAc < 1:3161327/71‑1 (MQ=255) cGCCAGTCGACGGAATTTTGCATTGCCGACCAgaagaa > 1:2942618/1‑38 (MQ=255) gTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg < 1:4721554/64‑1 (MQ=255) gTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCg < 1:4108227/64‑1 (MQ=255) gTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCagag < 1:3575149/71‑1 (MQ=255) gTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCagag < 1:3855766/71‑1 (MQ=255) gTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTa < 1:140067/40‑1 (MQ=255) gTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTa < 1:1274783/40‑1 (MQ=255) gTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCagag < 1:4293830/71‑1 (MQ=255) gTCGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCa > 1:921851/1‑68 (MQ=255) tCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATc < 1:1953425/41‑1 (MQ=255) cGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCa > 1:4166506/1‑66 (MQ=255) cGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCa > 1:1600952/1‑66 (MQ=255) cGACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATc > 1:691368/1‑40 (MQ=255) gACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGAt < 1:720035/70‑1 (MQ=255) gACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGAt < 1:3293002/70‑1 (MQ=255) gACGGCATTTTGCATTGCCGACCAAAAGAACCCGCTATCaa > 1:4272968/1‑41 (MQ=255) aTTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGcccc < 1:2124341/71‑1 (MQ=255) aTTTTGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGccc > 1:4675150/1‑70 (MQ=255) ttttGCATTGCCGACCAGAAGAACCCGCTATCAATGCCtt < 1:1091479/40‑1 (MQ=255) ttttGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTcc < 1:704280/43‑1 (MQ=255) ttttGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGAc < 1:3364743/56‑1 (MQ=255) ttGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGa > 1:4063127/1‑47 (MQ=255) ttGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGa > 1:4522884/1‑53 (MQ=255) ttGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGAACAGAGATCCGccc > 1:2852105/1‑67 (MQ=255) ttGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATc > 1:2272777/1‑62 (MQ=255) ttGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGccc > 1:3182645/1‑67 (MQ=255) ttGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAg < 1:2059950/70‑1 (MQ=255) ttGCATTGCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAg < 1:4362911/70‑1 (MQ=255) tGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAAc > 1:2589010/1‑43 (MQ=255) cATTGCCGACCAGAAGAACCCGCAATCAATGCCTTTcc < 1:4074650/38‑1 (MQ=255) ttGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACt < 1:4132208/40‑1 (MQ=255) tGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCa > 1:1202933/1‑71 (MQ=255) gCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGa > 1:3627464/1‑40 (MQ=255) gCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCa > 1:2921078/1‑70 (MQ=255) gCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCa < 1:1534748/70‑1 (MQ=255) gCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCg > 1:3556412/1‑57 (MQ=255) gCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCa > 1:2858444/1‑70 (MQ=255) gCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCAc > 1:4812578/1‑71 (MQ=255) gCCGACCAAAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCAc > 1:2504475/1‑71 (MQ=255) ccGACCAGAAGAA‑‑CGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGc < 1:4992537/71‑1 (MQ=255) gACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGc < 1:4379445/71‑1 (MQ=255) gACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGc < 1:3818279/71‑1 (MQ=255) gACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGc < 1:4838064/71‑1 (MQ=255) aCCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAgaga < 1:3589974/49‑1 (MQ=255) ccAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCag > 1:3632036/1‑45 (MQ=255) ccAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCag > 1:134838/1‑45 (MQ=255) ccAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAgaga < 1:2001483/48‑1 (MQ=255) agaagaACCCGCTATCAATGCCTTTCCAACTGACGcc < 1:4276481/37‑1 (MQ=255) | CACGCTCTTAATCAGACAGTTGACCAGATCCATACGCCAGTCGACGGCATTTTGCATTGCCGACCAGAAGAACCCGCTATCAATGCCTTTCCAACTGACGCCGACCAGAGATCCGCCCCAGATCCCCACGGC > W3110S.gb/3338483‑3338614 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |