| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,348,184 | T→C | 8.1% | intergenic (+91/‑123) | npr → / → yrbL | phosphohistidinoprotein‑hexose phosphotransferase component of N‑regulated PTS system (Npr)/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,348,184 | 0 | T | C | 8.1% | 313.3 / 16.7 | 111 | intergenic (+91/‑123) | npr/yrbL | phosphohistidinoprotein‑hexose phosphotransferase component of N‑regulated PTS system (Npr)/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (48/54); new base C (5/4); total (53/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01 | |||||||||||
Only 100 of 118 total aligned reads displayed.
CTCCCTTCAAACTCCCCCCGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTT > W3110S.gb/3348116‑3348254 | cTCCCTTCAAACTCCCCCCGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTa > 1:514320/1‑70 (MQ=255) tCCCTTCAAACTCCCCCCGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTAc > 1:279411/1‑70 (MQ=255) cTTCAAACTCCCCCCGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACAttt < 1:2379562/71‑1 (MQ=255) cTTCAAACTCCCCCCGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACAttt < 1:3115446/71‑1 (MQ=255) cTTCAAACTCCCCCCGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACAttt < 1:763840/71‑1 (MQ=255) cAAACTCCCCCCGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTtgt > 1:1233321/1‑71 (MQ=255) cAAACTCCCCCCGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTtgt > 1:2905943/1‑71 (MQ=255) cAAACTCCCCCCGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTtg < 1:4349657/70‑1 (MQ=255) tCCCCCCGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCt > 1:4603641/1‑71 (MQ=255) cccccGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCttt > 1:5121720/1‑71 (MQ=255) cccccGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCttt > 1:473304/1‑71 (MQ=255) cccccGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCtt < 1:4437932/70‑1 (MQ=255) cccccGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCtt < 1:125468/70‑1 (MQ=255) cccccGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCtt < 1:4543032/70‑1 (MQ=255) cccccGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCt < 1:2522778/69‑1 (MQ=255) cccccGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGCACATTTTGTGCGCttt < 1:2865362/71‑1 (MQ=255) ccccGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCtttt < 1:2828969/71‑1 (MQ=255) cccGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCtttt > 1:4540041/1‑70 (MQ=255) cccGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCtttt > 1:3097930/1‑70 (MQ=255) ccGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCtttt > 1:4181710/1‑69 (MQ=255) ccGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCtttt > 1:3947839/1‑69 (MQ=255) ccGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGCACATTTTGTGCGCTTTTCa > 1:603999/1‑71 (MQ=255) ccGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGCACATTTTGTGCGCTTTTCa < 1:249187/71‑1 (MQ=255) gACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCAc > 1:134129/1‑70 (MQ=255) gACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACt > 1:1312030/1‑71 (MQ=255) gACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACt < 1:4180748/71‑1 (MQ=255) tAAGAAAATCACGTACTGAAATCTTTCTCAATCAACGTCATTTGt < 1:2008052/45‑1 (MQ=255) tAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGt < 1:2967655/45‑1 (MQ=255) tAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGt < 1:1554487/45‑1 (MQ=255) tAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGt < 1:153672/45‑1 (MQ=255) tAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGt < 1:4423934/45‑1 (MQ=255) tAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTATGCGCtt > 1:2496649/1‑60 (MQ=255) tAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGCACATTTTGTGCGCTTTTCACttt > 1:1640631/1‑68 (MQ=255) tAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGCACATTTTGTGCGCTTTTCACttt > 1:4805707/1‑68 (MQ=255) aGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTa < 1:5100297/44‑1 (MQ=255) aGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTa < 1:3889617/44‑1 (MQ=255) aGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTa < 1:1011099/44‑1 (MQ=255) aGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTa < 1:1153138/44‑1 (MQ=255) aGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCaga < 1:688290/70‑1 (MQ=255) aGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCaga < 1:208398/70‑1 (MQ=255) aGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCag > 1:3081188/1‑69 (MQ=255) aGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCag > 1:399296/1‑69 (MQ=255) aGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCag > 1:4188113/1‑69 (MQ=255) gAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTc < 1:2235686/66‑1 (MQ=255) aaTCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAAc > 1:2054919/1‑71 (MQ=255) aCGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCtt > 1:518315/1‑50 (MQ=255) aCGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCtt > 1:3592566/1‑70 (MQ=255) cTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGaat < 1:1476831/71‑2 (MQ=255) cTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGaa < 1:4609752/70‑1 (MQ=255) cTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGaa > 1:3402392/1‑70 (MQ=255) cTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGaa > 1:733337/1‑70 (MQ=255) gAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCa > 1:2537461/1‑47 (MQ=255) gAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCag > 1:4142771/1‑54 (MQ=255) gAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAAc > 1:878796/1‑71 (MQ=255) aaaTCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCtttt > 1:4879412/1‑44 (MQ=255) aaaTCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAAcc > 1:4332549/1‑71 (MQ=255) aaTCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCagaag < 1:713047/55‑1 (MQ=255) aTCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCtt < 1:1830889/71‑1 (MQ=255) aTCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCtt < 1:641485/71‑1 (MQ=255) aTCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCtt < 1:1904567/71‑1 (MQ=255) tCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCtt < 1:619998/70‑1 (MQ=255) tCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCtt < 1:4493215/70‑1 (MQ=255) tCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCtt < 1:4291729/70‑1 (MQ=255) gTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTaa < 1:3513154/70‑1 (MQ=255) gTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTaa < 1:4046719/70‑1 (MQ=255) gTTCTCAATCAACGTCATTTGCACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTaa < 1:829735/70‑1 (MQ=255) ttCTCAATCAACGTCATTTCTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCtt < 1:4856024/67‑1 (MQ=255) tctcAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCtt < 1:1009989/66‑1 (MQ=255) tctcAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTaa < 1:3963119/68‑1 (MQ=255) ctcAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAgg > 1:2196632/1‑71 (MQ=255) tcaatcaaCGTCATTTGTACATTTTGTGCGCTTTTCACTTTc < 1:1885701/42‑1 (MQ=255) atcaaCGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCAtt > 1:3353483/1‑71 (MQ=255) tcaaCGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTaa < 1:1281390/62‑1 (MQ=255) tcaaCGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTg > 1:1739381/1‑71 (MQ=255) aaCGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTaa > 1:1458081/1‑49 (MQ=255) aaCGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTaa < 1:1662885/60‑1 (MQ=255) aaCGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTaa > 1:2153139/1‑60 (MQ=255) aaCGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGtt < 1:389932/71‑1 (MQ=255) aaCGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGtt < 1:150787/71‑1 (MQ=255) aCGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGttt < 1:505075/71‑1 (MQ=255) tCATTTGTACATTTTGTGCGCTTTTCACTTTCAGATGAACCTTAAGAAAACCTTAAGAGGCATTGtt < 1:4909449/67‑1 (MQ=255) tCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAgg < 1:1490944/71‑1 (MQ=255) tCATTTGCACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAag > 1:2582262/1‑57 (MQ=255) tCATTTGCACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAgg > 1:1944119/1‑71 (MQ=255) aTTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAagag > 1:893981/1‑57 (MQ=255) aTTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGtt > 1:2090194/1‑71 (MQ=255) aTTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGtt > 1:3250413/1‑71 (MQ=255) aTTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGtt > 1:3489954/1‑71 (MQ=255) aTTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGtt < 1:1524265/71‑1 (MQ=255) aTTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGtt > 1:4588128/1‑71 (MQ=255) tttGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTaa < 1:2617930/53‑1 (MQ=255) tttGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGttt < 1:324549/71‑1 (MQ=255) tttGCACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGttt < 1:2676947/71‑1 (MQ=255) tGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTg > 1:877768/1‑71 (MQ=255) gTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTaa < 1:4202520/39‑1 (MQ=255) gTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTa < 1:2079399/63‑1 (MQ=255) gTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTa < 1:356732/63‑1 (MQ=255) gTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAgg > 1:1559099/1‑65 (MQ=255) tACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGtt > 1:483195/1‑71 (MQ=255) tACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGtt < 1:2854466/71‑1 (MQ=255) | CTCCCTTCAAACTCCCCCCGACAATAAGAAAATCACGTACTGAAATCGTTCTCAATCAACGTCATTTGTACATTTTGTGCGCTTTTCACTTTCAGAAGAACCTTAAGAAAACCTTAAGAGGCATTGTTTAGGTTTTGTT > W3110S.gb/3348116‑3348254 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |