| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,375,433 | A→G | 30.2% | D237G (GAT→GGT) | dcuD → | predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,375,433 | 0 | A | G | 30.2% | 84.6 / 53.0 | 63 | D237G (GAT→GGT) | dcuD | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base A (19/25); new base G (11/8); total (30/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
GTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTC > W3110S.gb/3375366‑3375503 | gTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATa < 1:4374672/70‑1 (MQ=255)gTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATa < 1:2964646/70‑1 (MQ=255) gCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATg > 1:3888497/1‑71 (MQ=255) cAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGt > 1:122635/1‑71 (MQ=255) gcgcTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCccg > 1:2763013/1‑71 (MQ=255) tttGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGct > 1:2318998/1‑71 (MQ=255) tttGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATaa < 1:1806704/59‑1 (MQ=255) tttGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATaa < 1:2411552/59‑1 (MQ=255) tGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGctc < 1:2662332/70‑1 (MQ=255) tGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGctc < 1:3510279/70‑1 (MQ=255) gACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGctct < 1:1373639/70‑1 (MQ=255) gACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTa > 1:4667394/1‑71 (MQ=255) aCAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTAt < 1:2931568/71‑1 (MQ=255) aaaaaaGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAAt > 1:1684109/1‑54 (MQ=255) aaaGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGc > 1:654485/1‑71 (MQ=255) aaaGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTAATACGc > 1:417564/1‑71 (MQ=255) aaGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGcc < 1:1399226/71‑1 (MQ=255) aGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCa < 1:1297317/71‑1 (MQ=255) aGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCa < 1:5121554/71‑1 (MQ=255) aGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCa > 1:1863888/1‑71 (MQ=255) aGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCa > 1:712757/1‑71 (MQ=255) gATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCAt < 1:4908609/71‑1 (MQ=255) gATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCccgc > 1:1845516/1‑55 (MQ=255) tAAAAATATCAATCACGAACAGGCAGAGCAAAACGCTCTCGATAATGTCCCGCCGCTCTATTACGCCAttt > 1:3283646/1‑71 (MQ=255) aaaaTATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTa < 1:4175590/42‑1 (MQ=255) aaaaTATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTa < 1:5129943/42‑1 (MQ=255) tatCAATCACGAACAGGCAGAGCAAAAAGCTCTCGGTAATGTCCCGCCGctc < 1:5038946/52‑1 (MQ=255) caatcaCGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCACGCCGCTCTATTACGCCATTTTACCTGTGa > 1:4962070/1‑71 (MQ=255) atcaCGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATg < 1:2687800/71‑1 (MQ=255) tcaCGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGctct < 1:642192/47‑1 (MQ=255) aCGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCAtt > 1:1657923/1‑56 (MQ=255) aCGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCg > 1:641502/1‑71 (MQ=255) aCGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCg > 1:2137050/1‑71 (MQ=255) aGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATc > 1:1464616/1‑71 (MQ=255) agagCAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGa < 1:1933898/71‑1 (MQ=255) cAAAAAGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATgct > 1:3896359/1‑71 (MQ=255) cAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATgct < 1:3042368/71‑1 (MQ=255) aaaaaGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCATTTTAc > 1:1305759/1‑45 (MQ=255) aaaaGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATgctcg > 1:1792984/1‑71 (MQ=255) aaaaGCTCTCGATAATGTCCCGCCGCTCTATTACGCCAtttt < 1:3774727/42‑1 (MQ=255) aaaaGCTCTCGATAATGTCCCGCCGCTCTATTACGCCAtttt < 1:806232/42‑1 (MQ=255) aaaaGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATgctcg < 1:1157571/71‑1 (MQ=255) aaaaGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATgctcg > 1:3326921/1‑71 (MQ=255) aaaGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCATTTTACCtg > 1:3440307/1‑46 (MQ=255) aaaGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATcc < 1:3133302/61‑1 (MQ=255) aaaGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATgc < 1:2738429/67‑1 (MQ=255) aaGCTCTCGGTAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGa > 1:5142578/1‑63 (MQ=255) aGCTCTCGATAATGTCCCGCCGCTCTATTACTCCATTTTAc > 1:1436819/1‑41 (MQ=255) aGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTAc > 1:408999/1‑41 (MQ=255) aGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTAc > 1:2371317/1‑41 (MQ=255) aGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTAc > 1:2507334/1‑41 (MQ=255) aGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATgctc > 1:4837081/1‑67 (MQ=255) ctctCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGtt < 1:1009535/52‑1 (MQ=255) ctctCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGtt < 1:3366369/52‑1 (MQ=255) ctctCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGtt < 1:4359920/52‑1 (MQ=255) tcGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATgctcgcc < 1:3241699/65‑3 (MQ=255) cGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGt > 1:3150674/1‑71 (MQ=255) gATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGtt < 1:4413723/47‑1 (MQ=255) gATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGtt < 1:1455873/47‑1 (MQ=255) gATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGtt > 1:4131943/1‑47 (MQ=255) gATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGtt < 1:4059435/47‑1 (MQ=255) gATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGt < 1:5086156/70‑1 (MQ=255) gATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGt < 1:3607723/70‑1 (MQ=255) aTAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTc < 1:2014035/71‑1 (MQ=255) | GTGCAACGCGCTTTTGACAAAAAAGATAAAAATATCAATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTC > W3110S.gb/3375366‑3375503 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |