| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,439,867 | T→C | 35.6% | intergenic (‑89/‑6) | nudC ← / → rsd | NADH pyrophosphatase/stationary phase protein, binds sigma 70 RNA polymerase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,439,867 | 0 | T | C | 35.6% | 69.0 / 74.5 | 73 | intergenic (‑89/‑6) | nudC/rsd | NADH pyrophosphatase/stationary phase protein, binds sigma 70 RNA polymerase subunit |
| Reads supporting (aligned to +/- strand): ref base T (28/19); new base C (11/15); total (39/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAACTGGTTGATC > W3110S.gb/3439799‑3439933 | aTCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCa < 1:474109/71‑1 (MQ=255)aTCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCa < 1:4570221/71‑1 (MQ=255) cACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGCCAAt > 1:653112/1‑71 (MQ=255) aCTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAAt < 1:2484489/70‑1 (MQ=255) aCTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAAt > 1:1270039/1‑70 (MQ=255) aCTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATc < 1:1308518/71‑1 (MQ=255) aCTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATc < 1:265000/71‑1 (MQ=255) aCTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATc < 1:4903909/71‑1 (MQ=255) aCTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATc < 1:367703/71‑1 (MQ=255) aCTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGCCAAt > 1:2795199/1‑70 (MQ=255) cTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGCCAATCa < 1:229265/71‑1 (MQ=255) cTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGCCAATCa > 1:555302/1‑71 (MQ=255) gCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGc > 1:777949/1‑71 (MQ=255) gCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGCCAATCAt < 1:2402178/69‑1 (MQ=255) tGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTa < 1:1542107/71‑1 (MQ=255) tGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTa < 1:3919178/71‑1 (MQ=255) tttAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGCCAAt < 1:1431660/61‑1 (MQ=255) tttAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGCCAAt < 1:531149/61‑1 (MQ=255) ttAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGc > 1:939390/1‑65 (MQ=255) tGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAAt > 1:2719166/1‑54 (MQ=255) tGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGCCAATc < 1:4154706/55‑1 (MQ=255) gTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGa > 1:3958420/1‑70 (MQ=255) gTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGa > 1:1420087/1‑70 (MQ=255) gTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGa > 1:802472/1‑70 (MQ=255) gTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGa > 1:4573597/1‑70 (MQ=255) ttttACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATaa > 1:3389334/1‑71 (MQ=255) ttttACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAACTCGATaa > 1:3999490/1‑71 (MQ=255) tttACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCa < 1:1395910/47‑1 (MQ=255) tttACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCa < 1:2670842/47‑1 (MQ=255) tACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGCCAATCATGCTTAACCAGCTCGATAAcc > 1:76992/1‑70 (MQ=255) cATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTg > 1:3521607/1‑70 (MQ=255) cATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGa > 1:4585369/1‑71 (MQ=255) cATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGa < 1:4565029/71‑1 (MQ=255) cATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGa > 1:4846674/1‑71 (MQ=255) aTTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGAc < 1:4438171/71‑1 (MQ=255) tttCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCg < 1:2803713/60‑1 (MQ=255) tttCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCg < 1:406799/60‑1 (MQ=255) tttCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGCCAATCATGCTTAACCAGCTCGGTAACCTGACg < 1:1578299/71‑1 (MQ=255) ttCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACg > 1:4958929/1‑70 (MQ=255) ttCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGCCAATCATGCTTAACCAGCTCGATAACCTGACg > 1:2973201/1‑70 (MQ=255) ttCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGCCAATCATGCTTAACCAGCTCGATAACCTGACg > 1:2099983/1‑70 (MQ=255) tctcACTGAGCAGTTTTTGAATACAAACTTGCGGAGCCAATCATGCTTAACCAGCTCGATAACCTGACg > 1:1237657/1‑69 (MQ=255) tcACTGAGCAGTTTTTGAATACAAACTTGCGGAGCCAATCATGCTTAACCAGCTc < 1:2349685/55‑1 (MQ=255) cACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCa < 1:4486310/36‑1 (MQ=255) aCTGAGCAGTTTTTGAATACAAACTTGCGGAGCCAATCATGCTTAACCAGCTCGa > 1:1929998/1‑55 (MQ=255) aCTGAGCAGTTTTTGAATACAAACTTGCGGAGCCAATCATGCTTAACCAGCTCGa > 1:4642623/1‑55 (MQ=255) tGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGt > 1:3195372/1‑71 (MQ=255) tGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGt > 1:3316843/1‑71 (MQ=255) tGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGt > 1:3128221/1‑71 (MQ=255) tGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGt > 1:2515654/1‑71 (MQ=255) gCAGTTTTTGAATACAAACTTGCGGAGCCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCag < 1:4975652/71‑1 (MQ=255) tttttGAATACAAACTTGCGGAGTCAATCATGCTTa < 1:4068737/36‑1 (MQ=255) tttttGAATACAAACTTGCGGAGCCAATCATGCTTa < 1:3816393/36‑1 (MQ=255) tttttGAATACAAACTTGCGGAGCCAATCATGCTTa < 1:1219651/36‑1 (MQ=255) tttttGAATACAAACTTGCGGAGCCAATCATGCTTa < 1:3165748/36‑1 (MQ=255) tttttGAATACAAACTTGCGGAGCCAATCATGCTTa < 1:789838/36‑1 (MQ=255) tttttGAATACAAACTTGCGGAGCCAATCATGCTTa < 1:4862624/36‑1 (MQ=255) tttctGAATACAAACTTGCGGAGCCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTc < 1:5072235/65‑1 (MQ=255) ttttGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATa < 1:2291218/47‑1 (MQ=255) ttttGAATACAAACTTGCGGAGCCAATCATGCTTAAc > 1:4212510/1‑37 (MQ=255) tttGAATACAAACTTGCGGAGTCAATCATGCTTaa > 1:2058327/1‑35 (MQ=255) tttGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAg > 1:525141/1‑71 (MQ=255) tttGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAg > 1:3725801/1‑71 (MQ=255) tttGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAg > 1:2850528/1‑71 (MQ=255) aTACAAACTTGCGGAGCCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACa < 1:4205547/71‑1 (MQ=255) tACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACaa < 1:1278358/71‑1 (MQ=255) cAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACg > 1:2513516/1‑46 (MQ=255) cAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAg > 1:3525043/1‑49 (MQ=255) cAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAg > 1:1858755/1‑49 (MQ=255) cAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAAc > 1:4489748/1‑71 (MQ=255) cAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAAc > 1:4090777/1‑71 (MQ=255) aCTTGCGGAGCCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAACTgg > 1:2820948/1‑71 (MQ=255) cTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAACTGGt > 1:4344336/1‑71 (MQ=255) tCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAACTGGTTGATc < 1:997571/67‑1 (MQ=255) | ATCACTGGCATGTTTAACATGGTTTTTACATTTCTCACTGAGCAGTTTTTGAATACAAACTTGCGGAGTCAATCATGCTTAACCAGCTCGATAACCTGACGGAGCGCGTCAGAGGAAGTAACAAACTGGTTGATC > W3110S.gb/3439799‑3439933 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |