| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,458,921 | T→C | 42.6% | K6K (AAA→AAG) | nusG ← | transcription termination factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,458,921 | 0 | T | C | 42.6% | 30.0 / 85.2 | 68 | K6K (AAA→AAG) | nusG | transcription termination factor |
| Reads supporting (aligned to +/- strand): ref base T (27/12); new base C (14/15); total (41/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATACCAT > W3110S.gb/3458853‑3458989 | tGCTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGcttctt < 1:4332719/71‑1 (MQ=255)tGCTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCtttt < 1:464384/70‑1 (MQ=255) cTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCttttt > 1:3877885/1‑69 (MQ=255) cTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCttttt > 1:1562635/1‑69 (MQ=255) aCGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAg > 1:3137916/1‑68 (MQ=255) aCGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAg > 1:3131710/1‑68 (MQ=255) gCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAg > 1:3393248/1‑66 (MQ=255) gCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAg > 1:4373115/1‑66 (MQ=255) cAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCtt < 1:2785390/71‑1 (MQ=255) gACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGa > 1:835629/1‑71 (MQ=255) aCGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGAc < 1:2920771/71‑1 (MQ=255) gCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTa > 1:3742858/1‑54 (MQ=255) gCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTa > 1:5073908/1‑54 (MQ=255) gCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAg > 1:4147067/1‑55 (MQ=255) gCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAg > 1:2611097/1‑55 (MQ=255) gCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACAtct > 1:3919600/1‑70 (MQ=255) gCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACAtct > 1:3483874/1‑70 (MQ=255) gCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACAtct > 1:1539442/1‑70 (MQ=255) gCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACAtct > 1:4348803/1‑70 (MQ=255) cgcgGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGaa > 1:1840327/1‑71 (MQ=255) cgcgGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGaa < 1:1027038/71‑1 (MQ=255) cgcgGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGa < 1:3468602/70‑1 (MQ=255) cgcgGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAg < 1:4108184/69‑1 (MQ=255) cgcgGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGaa > 1:3326013/1‑71 (MQ=255) cgcgGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGa < 1:3003086/70‑1 (MQ=255) ccTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCt > 1:3352055/1‑69 (MQ=255) cTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAg < 1:4348064/54‑1 (MQ=255) ttCAAAACCGGAAAACGCCTGAACGACGTACCAGCGcttctt < 1:4327800/42‑1 (MQ=255) ttCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCa < 1:1950566/52‑1 (MQ=255) tCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGAc > 1:1791910/1‑54 (MQ=255) tCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGc > 1:4325674/1‑71 (MQ=255) tCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGc > 1:4746088/1‑71 (MQ=255) tCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGc > 1:1183883/1‑71 (MQ=255) tCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGc > 1:2728983/1‑71 (MQ=255) cAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAg > 1:4917658/1‑42 (MQ=255) cAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGc > 1:4711135/1‑70 (MQ=255) cAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAgg > 1:634743/1‑43 (MQ=255) cAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAg > 1:4186903/1‑45 (MQ=255) cAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAg > 1:4635951/1‑45 (MQ=255) cAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGa > 1:3791125/1‑52 (MQ=255) aaaaCCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTa > 1:2378759/1‑40 (MQ=255) aaaaCCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGcc < 1:3615988/70‑1 (MQ=255) aaaaCCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGcc < 1:3645875/70‑1 (MQ=255) aaaaCCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGc > 1:3942340/1‑69 (MQ=255) aCCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTg < 1:2063311/71‑1 (MQ=255) aCCGGAAAACGCCTGAACGACGTACCAGCGCTTCTTAgg < 1:4271497/39‑1 (MQ=255) ggAAAACGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATa < 1:1915742/71‑1 (MQ=255) aaaCGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGAt > 1:1592595/1‑67 (MQ=255) aaaCGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAg < 1:3340241/71‑1 (MQ=255) aaaCGCCTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAg > 1:2668778/1‑71 (MQ=255) aaCGCCAGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAg > 1:4584/1‑70 (MQ=255) ccTGAACGACGTACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAg < 1:4832532/66‑1 (MQ=255) tGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATAcc < 1:2634571/70‑1 (MQ=255) acgTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCt > 1:3106490/1‑42 (MQ=255) acgTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGaa > 1:3248535/1‑71 (MQ=255) acgTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGaa > 1:1809168/1‑71 (MQ=255) tACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCg < 1:4700710/66‑1 (MQ=255) tACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCa < 1:62103/71‑1 (MQ=255) tACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCa < 1:1095103/71‑1 (MQ=255) tACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCa < 1:4006862/71‑1 (MQ=255) tACCAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCa < 1:104278/71‑1 (MQ=255) ccAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATa > 1:179340/1‑57 (MQ=255) ccAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGc > 1:3382160/1‑63 (MQ=255) ccAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAg < 1:5144942/70‑1 (MQ=255) ccgcgcTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAg < 1:3371355/67‑1 (MQ=255) cAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGa > 1:4246580/1‑54 (MQ=255) cAGCGCTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAg < 1:5139877/69‑1 (MQ=255) aGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGaa > 1:4047698/1‑70 (MQ=255) cgcTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATa > 1:4842895/1‑70 (MQ=255) gcTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATAcc < 1:4622559/71‑1 (MQ=255) gcTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATAcc < 1:2901116/71‑1 (MQ=255) gcTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATAcc < 1:3608590/71‑1 (MQ=255) gcTTCTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATAc > 1:754848/1‑70 (MQ=255) tttAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATACCAt < 1:103448/69‑1 (MQ=255) | TGCTCACGCAGCGACGTTGCTACGCGGCCTTCAAAACCGGAAAACGCCTGAACGACGTACCAGCGCTTTTTAGGAGCTTCAGACATCTCAGAACCTCAGGCCAGTGATAAAGGATACCAGGCGAACCAGAATACCAT > W3110S.gb/3458853‑3458989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |