| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,459,280 | T→C | 33.3% | E15G (GAA→GGA) | secE ← | preprotein translocase membrane subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,459,280 | 0 | T | C | 33.3% | 49.3 / 45.1 | 48 | E15G (GAA→GGA) | secE | preprotein translocase membrane subunit |
| Reads supporting (aligned to +/- strand): ref base T (15/17); new base C (12/4); total (27/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
GCGATAAAGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGTT > W3110S.gb/3459213‑3459343 | gcgATAAAGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCa < 1:386449/71‑1 (MQ=255)gcgATAAAGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCa < 1:3404763/71‑1 (MQ=255) cgATAAAGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTcc < 1:3801087/69‑1 (MQ=255) gATAAAGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAgg < 1:4357972/71‑1 (MQ=255) aaaGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCcg > 1:2937002/1‑71 (MQ=255) gCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTc > 1:5067171/1‑71 (MQ=255) gCCGACAATCGCCACCAGGAGCAATGCAACCACAACGACACACTTCATCGCTCCCAGGCCGCGCCCGCTTc > 1:2435920/1‑71 (MQ=255) ccGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTc < 1:362587/70‑1 (MQ=255) aCAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTg < 1:4560914/71‑1 (MQ=255) tCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAg > 1:5132015/1‑69 (MQ=255) caccaGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCgg < 1:4114313/71‑1 (MQ=255) caccaGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCgg < 1:1719125/71‑1 (MQ=255) accaGGAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCgg > 1:4142131/1‑70 (MQ=255) ccaGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCcgcg < 1:268399/49‑1 (MQ=255) caGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTAt < 1:4698300/71‑1 (MQ=255) aGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTAtt > 1:3832578/1‑71 (MQ=255) gAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCg > 1:1893365/1‑71 (MQ=255) gAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCtt < 1:4607513/52‑1 (MQ=255) gAGCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCtt < 1:4185278/52‑1 (MQ=255) gCAATGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCa > 1:3037216/1‑71 (MQ=255) aaTGCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTa < 1:3213220/63‑1 (MQ=255) tGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTg < 1:1658051/51‑1 (MQ=255) tGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTg < 1:1895629/51‑1 (MQ=255) tGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAg > 1:1670638/1‑53 (MQ=255) tGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAACTTCGGTATTCGCACTCa < 1:4821060/71‑1 (MQ=255) tGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCACGCTTCCTTGAGCTTCGGTATTCGCACTCa > 1:3467861/1‑71 (MQ=255) gCCACCACAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCa > 1:3716435/1‑70 (MQ=255) ccaccaCAACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCAt > 1:3970578/1‑70 (MQ=255) acaACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTc < 1:1808146/40‑1 (MQ=255) caACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAaaccaa > 1:1773206/1‑71 (MQ=255) caACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAaaccaa > 1:4340333/1‑71 (MQ=255) caACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAaaccaa > 1:2002622/1‑71 (MQ=255) caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCa > 1:646799/1‑59 (MQ=255) caACGACCCACTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCa > 1:1924642/1‑59 (MQ=255) aaCGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTc < 1:4726389/48‑1 (MQ=255) aaCGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTc < 1:1270511/48‑1 (MQ=255) aCGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAaaccaacc > 1:5121262/1‑71 (MQ=255) gACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGa > 1:2482804/1‑40 (MQ=255) gACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTg > 1:4169478/1‑71 (MQ=255) aCTTCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCa > 1:2122412/1‑50 (MQ=255) tCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGt > 1:2814160/1‑71 (MQ=255) tCATCGCTCCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGt > 1:4650708/1‑71 (MQ=255) aTCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGtt < 1:2726619/70‑1 (MQ=255) tCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCa < 1:1683731/48‑1 (MQ=255) cGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTcc > 1:626062/1‑40 (MQ=255) gCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTAtt > 1:2774217/1‑38 (MQ=255) gCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTAtt > 1:2311119/1‑38 (MQ=255) gCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCa > 1:3509396/1‑46 (MQ=255) | GCGATAAAGATAGTTGCCGACAATCGCCACCAGGAGCAATGCCACCACAACGACCCACTTCATCGCTTCCAGGCCGCGCCCGCTTCCTTGAGCTTCGGTATTCGCACTCATAAACCAACCTGTCAGAAGTT > W3110S.gb/3459213‑3459343 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |