| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,472,611 | G→A | 35.3% | G144G (GGC→GGT) | btuB ← | vitamin B12/cobalamin outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,472,611 | 0 | G | A | 35.3% | 35.2 / 30.1 | 34 | G144G (GGC→GGT) | btuB | vitamin B12/cobalamin outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base G (11/11); new base A (8/4); total (19/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CCCACCCTGCTGAAATTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGAC > W3110S.gb/3472550‑3472673 | cccACCCTGCTGAAATTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCa > 1:20440/1‑71 (MQ=255)cccACCCTGCTGAAATTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCACCTATTGCa > 1:4526959/1‑71 (MQ=255) ctgctgAAATTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGaa > 1:2895332/1‑71 (MQ=255) gAAATTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCAt > 1:1056078/1‑70 (MQ=255) gAAATTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCAt > 1:4560620/1‑70 (MQ=255) aaaTTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCAt > 1:1469952/1‑60 (MQ=255) aaaTTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATa < 1:5009425/70‑1 (MQ=255) aaaTTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATa < 1:1767206/70‑1 (MQ=255) aaaTTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATa < 1:1767627/70‑1 (MQ=255) tttCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACa < 1:4708347/71‑1 (MQ=255) cgttccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCg < 1:505461/70‑1 (MQ=255) gttccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCg > 1:350622/1‑69 (MQ=255) gttccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCg > 1:1593754/1‑69 (MQ=255) ttccgGGTTCATCGCGCGGCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAg < 1:4236116/71‑1 (MQ=255) tccgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAgc > 1:4607978/1‑71 (MQ=255) cgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAgcgc < 1:2591507/71‑1 (MQ=255) cgGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAgcgc < 1:1889115/71‑1 (MQ=255) tCGCGCGTCGTGATTATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACg < 1:1142009/71‑1 (MQ=255) tCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATa < 1:1251589/50‑1 (MQ=255) gcgcgTCGTGATGATATTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGa > 1:3262583/1‑57 (MQ=255) tgatgaTATTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTc < 1:3155712/70‑1 (MQ=255) tgatgaTATTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCa < 1:2492819/71‑1 (MQ=255) atatTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCa > 1:4960626/1‑54 (MQ=255) atatTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACg > 1:3390439/1‑71 (MQ=255) atatTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACg > 1:3197616/1‑71 (MQ=255) atatTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACg > 1:163783/1‑71 (MQ=255) atatTCACCACCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACg > 1:798053/1‑71 (MQ=255) tatTCACCACCCCGCCTATTGCATCGGAACCATAAACAg < 1:5081492/39‑1 (MQ=255) caccacCCCGCCTATTGCATCGGAACCATAAACAGCGGat > 1:2809813/1‑39 (MQ=255) caccacCCCGCCTATTGCATCGGAACCATAAACAGCGGAg > 1:3097151/1‑40 (MQ=255) caccacCCCGCCTATTGCATCGGAACCATAAACAGCGGAGcgcg > 1:5047924/1‑44 (MQ=255) caccacCCCACCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGa > 1:3537930/1‑71 (MQ=255) accaccCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGa < 1:70522/70‑1 (MQ=255) accaccCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGAc < 1:4785761/71‑1 (MQ=255) | CCCACCCTGCTGAAATTTCCGTTCCGGGTTCATCGCGCGTCGTGATGATATTCACCACCCCGCCTATTGCATCGGAACCATAAACAGCGGAGCGCGGCCCACGGATATATTCAACACGCTGGAC > W3110S.gb/3472550‑3472673 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |